SPC1 regelt de signaal-peptidase bemiddelde verwerken van membraan proteïnen
Signaalpeptidase (SPase) splitst de signaalsequenties (SS’s ) van secretoire voorlopers. Het bevat een evolutionair geconserveerde membraaneiwitsubeenheid, Spcl, die niet nodig is voor de katalytische activiteit van SPase, en de rol ervan blijft onbekend. In de huidige studie hebben we de functie van gist Spc1 onderzocht. Eerst hebben we een in vivo SPase-splitsingstest opgezet met behulp van secretoire eiwit-CPY- varianten met SS’s gemodificeerd in de n- en h-regio’s.
Bij het vergelijken van de SS-splitsingsefficiëntie van deze varianten in cellen met of zonder Spcl, ontdekten we dat signaalverankerde sequenties vatbaarder werden voor splitsing door SPase zonder Spcl. Verder was SPase-gemedieerde verwerking van modelmembraaneiwitten verbeterd in de afwezigheid van maar verminderd bij overexpressie van Spcl. Spcl werd geco-immunoprecipiteerd met eiwitten die niet-gesplitste signaalverankerde of transmembraan (TM) segmenten droegen.
Samengevat suggereren deze resultaten dat Spc1 TM-segmenten beschermt tegen SPase-actie, waardoor de substraatselectie voor SPase wordt verscherpt en fungeert als een negatieve regulator voor de SPase-gemedieerde verwerking van membraaneiwitten .
Interleukine-10 speelt een belangrijke rol in pasgeboren ratten met Hypoxic-ischemie geassocieerd met B-cel lymfoom 2 en endoplasmatisch reticulum Protein 29
Interleukine 10 (IL-10) is een synthetische remmer van humane cytokines met immunomodulerende en ontstekingsremmende effecten. Deze studie was bedoeld om de expressievariatie van IL-10 op meerdere plaatsen, waaronder cortex, hippocampus en longweefsels van neonatale hypoxisch-ischemische (HI) ratten te onderzoeken en de cruciale rol van IL-10 bij het verlichten van HI-hersenschade te onderzoeken. In deze studie werden neonatale Sprague-Dawley-ratten onderworpen aan de ligatie van de rechter gemeenschappelijke halsslagader, gevolgd door 2 uur hypoxie.
De expressie van IL-10 in de cortex, hippocampus en longweefsels werd gemeten met immunohistochemie, realtime kwantitatieve polymerasekettingreactie (RT-qPCR) en western blot (WB). Dubbele kleuring met immunofluorescentie werd uitgevoerd om de lokalisatie van IL-10 in neuronen en astrocyten te observeren . Bovendien werden niet-targeting en concentrating on IL-10 siRNA-lentivirusvectoren geïnjecteerd in respectievelijk de ratten van de negatieve controle (NC) en IL-10-groep, en de mRNA-niveaus van B-cellymfoom 2 (Bcl-2) en endoplasmatische reticulum eiwit 29 (ERp29) werden gedetecteerd door RT-qPCR na IL-10 stilte.
De resultaten toonden aan dat de IL-10-expressie aanzienlijk was verhoogd nadat HI en IL-10 waren gecolokaliseerd met neuronen en astrocyten die ernstig waren beschadigd door HI-belediging. Bovendien waren Bcl-2 en ERp29 opmerkelijk verlaagd na IL-10-mRNA-interferentie in vergelijking met de NC-groep. Onze bevindingen onthulden dat IL-10 zijn anti-apoptotische en neuroprotectieve effecten uitoefende door de expressie van Bcl-2 en ERp29 te reguleren, wat aangeeft dat IL-10 een veelbelovend molecuuldoelwit kan zijn voor HIE-behandeling.
) GCFC2 Recombinant Protein (Rat) |
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| RP202352 | ABM | 100 ug | Ask for price |
 Mouse GCFC2 shRNA Plasmid |
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| 20-abx983608 | Abbexa |
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 Human GCFC2 shRNA Plasmid |
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| 20-abx954755 | Abbexa |
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 Polyclonal Goat anti-GST α-form |
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| GST-ANTI-1 | Detroit R&D | 50 uL | EUR 280 |
 Polyclonal Goat anti-GST μ-form |
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| GST-ANTI-2 | Detroit R&D | 50 uL | EUR 280 |
 Polyclonal Goat anti-GST p-form |
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| GST-ANTI-3 | Detroit R&D | 50 uL | EUR 280 |
 (pORF)) Gcfc2 ORF Vector (Rat) (pORF) |
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| ORF067452 | ABM | 1.0 ug DNA | EUR 506 |
 (pORF)) GCFC2 ORF Vector (Human) (pORF) |
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| ORF020089 | ABM | 1.0 ug DNA | Ask for price |
 Antibody) Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody |
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| 20-abx323787 | Abbexa |
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Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody |
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| abx330966-100ul | Abbexa | 100 ul | EUR 425 |
Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody |
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| 20-abx008656 | Abbexa |
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 (pPB-C-His)) GCFC2 Protein Vector (Rat) (pPB-C-His) |
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| PV269806 | ABM | 500 ng | EUR 1166 |
 (pPB-N-His)) GCFC2 Protein Vector (Rat) (pPB-N-His) |
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| PV269807 | ABM | 500 ng | EUR 1166 |
 (pPM-C-HA)) GCFC2 Protein Vector (Rat) (pPM-C-HA) |
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| PV269808 | ABM | 500 ng | EUR 1166 |
 (pPM-C-His)) GCFC2 Protein Vector (Rat) (pPM-C-His) |
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| PV269809 | ABM | 500 ng | EUR 1166 |
 (pPB-C-His)) GCFC2 Protein Vector (Human) (pPB-C-His) |
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| PV080353 | ABM | 500 ng | Ask for price |
 (pPB-N-His)) GCFC2 Protein Vector (Human) (pPB-N-His) |
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| PV080354 | ABM | 500 ng | Ask for price |
 (pPM-C-HA)) GCFC2 Protein Vector (Human) (pPM-C-HA) |
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| PV080355 | ABM | 500 ng | Ask for price |
 (pPM-C-His)) GCFC2 Protein Vector (Human) (pPM-C-His) |
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| PV080356 | ABM | 500 ng | Ask for price |
 (CMV) (pLenti-GIII-CMV)) GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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| LV762095 | ABM | 1.0 ug DNA | EUR 920 |
 (UbC) (pLenti-GIII-UbC)) GCFC2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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| LV762099 | ABM | 1.0 ug DNA | EUR 920 |
 (EF1a) (pLenti-GIII-EF1a)) GCFC2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV762100 | ABM | 1.0 ug DNA | EUR 920 |
 (CMV) (pLenti-GIII-CMV)) GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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| LV654355 | ABM | 1.0 ug DNA | EUR 1355 |
 (UbC) (pLenti-GIII-UbC)) GCFC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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| LV654359 | ABM | 1.0 ug DNA | EUR 1355 |
 (EF1a) (pLenti-GIII-EF1a)) GCFC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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| LV654360 | ABM | 1.0 ug DNA | EUR 1355 |
 ELISA Kit) Human Chromosome 2 Open Reading Frame 3 (GCFC2) ELISA Kit |
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| abx386199-96tests | Abbexa | 96 tests | EUR 911 |
 (CMV) (pLenti-GIII-CMV-C-term-HA)) GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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| LV762096 | ABM | 1.0 ug DNA | EUR 920 |
 (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)) GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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| LV762097 | ABM | 1.0 ug DNA | EUR 978 |
 (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)) GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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| LV762098 | ABM | 1.0 ug DNA | EUR 978 |
 (CMV) (pLenti-GIII-CMV-C-term-HA)) GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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| LV654356 | ABM | 1.0 ug DNA | EUR 1355 |
 (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)) GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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| LV654357 | ABM | 1.0 ug DNA | EUR 1413 |
 (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)) GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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| LV654358 | ABM | 1.0 ug DNA | EUR 1413 |
 Anti-Anti-SEPT2 Antibody antibody |
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| STJ25475 | St John's Laboratory | 100 µl | EUR 277 |
 Anti-Anti-SEPT5 Antibody antibody |
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| STJ25477 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
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 Anti-Anti-SEPT8 Antibody antibody |
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| STJ25479 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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Anti-Anti-SEPT2 Antibody antibody |
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| STJ28365 | St John's Laboratory | 100 µl | EUR 277 |
 Anti-Anti-SEPT7 Antibody antibody |
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| STJ28963 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
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Anti-Anti-SEPT8 Antibody antibody |
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| STJ117206 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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 Anti-Anti-SEPT12 Antibody antibody |
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| STJ117759 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. |
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 Anti-Anti-MARCH6 Antibody antibody |
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| STJ118549 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-MARCH6 Antibody antibody |
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| STJ118550 | St John's Laboratory | 100 µl | EUR 277 |
 Anti-Anti-MARCH7 Antibody antibody |
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| STJ118752 | St John's Laboratory | 100 µl | EUR 277 |
 Anti-Anti-SEPT3 Antibody antibody |
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| STJ118990 | St John's Laboratory | 100 µl | EUR 277 |
 Anti-Anti-SEPT1 antibody antibody |
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| STJ119580 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012] |
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Anti-Anti-SEPT7 Antibody antibody |
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| STJ116214 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
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 Anti-Anti-SEPT6 antibody antibody |
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| STJ11100949 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. |
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 Anti-Anti-SEPT9 Antibody antibody |
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| STJ111369 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described. |
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 Anti-Anti-SEPT11 Antibody antibody |
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| STJ111530 | St John's Laboratory | 100 µl | EUR 277 |
 Anti-Anti-SEPT4 Antibody antibody |
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| STJ112276 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. |
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 Anti-Anti-MARCH9 Antibody antibody |
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| STJ112609 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT11 Antibody antibody |
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| STJ113941 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT11 Antibody antibody |
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| STJ114081 | St John's Laboratory | 100 µl | EUR 277 |
Anti-Anti-SEPT5 Antibody antibody |
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| STJ114819 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
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 Anti-Anti-MARCH8 Antibody antibody |
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| STJ114828 | St John's Laboratory | 100 µl | EUR 277 |
 Anti-Anti-DDB1 Antibody |
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| A00333 | BosterBio | 100uL | EUR 455 |
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Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse. |
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 ELISA Kit) Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5647.8 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 552.76 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 746.8 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3060.6 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| 4-AEA465Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. |
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) ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody) |
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| ELK8071 | ELK Biotech | 1 plate of 96 wells | EUR 432 |
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Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
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 Anti- ZNF660 antibody |
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| ABD2410 | Lifescience Market | 100 ug | EUR 438 |
 Anti-STAT5B Antibody |
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| A1753-100 | Biovision | EUR 479 | |
 Anti-CREB1 Antibody |
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| A1754-100 | Biovision | EUR 479 | |
 Anti-COX7B Antibody |
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| A1756-100 | Biovision | EUR 479 | |
 Anti-COX6B1 Antibody |
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| A1757-100 | Biovision | EUR 479 | |
 Anti-COX7B2 Antibody |
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| A1758-100 | Biovision | EUR 479 | |
 Anti-COX19 Antibody |
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| A1759-100 | Biovision | EUR 479 | |
 Anti-AIMP2 Antibody |
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| A1760-100 | Biovision | EUR 479 | |
 Anti-NFKBIE Antibody |
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| A1762-100 | Biovision | EUR 479 | |
 Anti-NFKBID Antibody |
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| A1764-100 | Biovision | EUR 479 | |
 Anti-APIP Antibody |
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| A1766-100 | Biovision | EUR 479 | |
 Anti-BAG1 Antibody |
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| A1768-100 | Biovision | EUR 479 | |
 Anti-IL10RA Antibody |
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| A1770-100 | Biovision | EUR 479 | |
 Anti-IL10RB Antibody |
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| A1771-100 | Biovision | EUR 479 | |
 Anti-IL12RB1 Antibody |
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| A1772-100 | Biovision | EUR 479 | |
 Anti-IL13RA1 Antibody |
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| A1773-100 | Biovision | EUR 479 | |
 Anti-IL13RA2 Antibody |
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| A1774-100 | Biovision | EUR 479 | |
 Anti-IL17RC Antibody |
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| A1775-100 | Biovision | EUR 479 | |
 Anti-IL18R1 Antibody |
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| A1776-100 | Biovision | EUR 479 | |
 Anti-IL1R1 Antibody |
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| A1777-100 | Biovision | EUR 479 | |
 Anti- TauD Antibody |
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| A1804-100 | Biovision | EUR 370 | |
Anti- TauD Antibody |
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| A1804-30T | Biovision | EUR 146 | |
 Anti-KRT20 Antibody |
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| A1859-100 | Biovision | EUR 403 | |
 Anti-PGR Antibody |
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| A1860-100 | Biovision | EUR 403 | |
 Anti-HSPA9 Antibody |
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| A1861-100 | Biovision | EUR 403 | |
 Anti-ERBB4 Antibody |
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| A1862-100 | Biovision | EUR 403 | |
 Anti-CA12 Antibody |
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| A1865-100 | Biovision | EUR 403 | |
 Anti-TPD52 Antibody |
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| A1867-100 | Biovision | EUR 403 | |
 Anti-STRADA Antibody |
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| A1868-100 | Biovision | EUR 403 | |
 Anti-CD276 Antibody |
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| A1869-100 | Biovision | EUR 403 | |
 Anti-ICT1 Antibody |
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| A1872-100 | Biovision | EUR 403 | |
 Anti-OCIAD1 Antibody |
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| A1873-100 | Biovision | EUR 403 | |
 Anti-BRINP1 Antibody |
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| A1874-100 | Biovision | EUR 403 | |
 Anti-WT1 Antibody |
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| A1875-100 | Biovision | EUR 403 | |
 Anti-SART3 Antibody |
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| A1879-100 | Biovision | EUR 403 | |
 Anti-RRBP1 Antibody |
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| A1880-100 | Biovision | EUR 403 | |
 Anti-KIAA1524 Antibody |
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| A1881-100 | Biovision | EUR 403 | |
 Anti-ENO2 Antibody |
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| A1883-100 | Biovision | EUR 403 | |
 Anti-GPC3 Antibody |
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| A1884-100 | Biovision | EUR 403 | |
 Anti-ACP1 Antibody |
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| A1885-100 | Biovision | EUR 403 | |
 Anti-Elafin Antibody |
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| A1887-100 | Biovision | EUR 403 | |
 Anti-AVEN Antibody |
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| A1891-100 | Biovision | EUR 403 | |
 Anti-MST1 Antibody |
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| A1895-100 | Biovision | EUR 403 | |
 Anti-WSB2 Antibody |
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| A1896-100 | Biovision | EUR 403 | |
 Anti-UPP1 Antibody |
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| A1898-100 | Biovision | EUR 403 | |
 Anti-YY1AP1 Antibody |
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| A1899-100 | Biovision | EUR 403 | |
 Anti-CDK5RAP3 Antibody |
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| A1907-100 | Biovision | EUR 403 | |
 Anti-TCERG1 Antibody |
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| A1909-100 | Biovision | EUR 403 | |
 Anti-CD44 Antibody |
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| A1910-100 | Biovision | EUR 403 | |
 Anti-TIMM17A Antibody |
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| A1911-100 | Biovision | EUR 403 | |
 Anti-CYP2W1 Antibody |
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| A1912-100 | Biovision | EUR 403 | |
 Anti-NDUFAF2 Antibody |
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| A1913-100 | Biovision | EUR 403 | |
 Anti-PRAME Antibody |
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| A1914-100 | Biovision | EUR 403 | |
 Anti-S100P Antibody |
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| A1915-100 | Biovision | EUR 403 | |
 Anti-HES3 Antibody |
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| A1636-100 | Biovision | EUR 479 | |
 Anti-HES5 Antibody |
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| A1637-100 | Biovision | EUR 479 | |
 Anti-HMGA2 Antibody |
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| A1638-100 | Biovision | EUR 479 | |
 Anti-HuD Antibody |
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| A1639-100 | Biovision | EUR 370 | |
 Anti-ITGA4 Antibody |
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| A1640-100 | Biovision | EUR 479 | |
 Anti-IPMK Antibody |
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| A1641-100 | Biovision | EUR 479 | |
 Anti-LHX2 Antibody |
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| A1643-100 | Biovision | EUR 479 | |
 Anti-MAP2 Antibody |
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| A1644-100 | Biovision | EUR 479 | |
 Anti-MSI1 Antibody |
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| A1646-100 | Biovision | EUR 479 | |
 Anti-NEDD1 Antibody |
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| A1647-100 | Biovision | EUR 370 | |
 Anti-NEUROD2 Antibody |
|||
| A1648-100 | Biovision | EUR 370 | |
 Anti-NGFR Antibody |
|||
| A1650-100 | Biovision | EUR 479 | |
 Anti-OLFM1 Antibody |
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| A1651-100 | Biovision | EUR 370 | |
 Anti-NR2E1 Antibody |
|||
| A1654-100 | Biovision | EUR 479 | |
 Anti-OLIG2 Antibody |
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| A1656-100 | Biovision | EUR 479 | |
 Anti-PAX3 Antibody |
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| A1657-100 | Biovision | EUR 370 | |
 Anti-PLAGL1 Antibody |
|||
| A1658-100 | Biovision | EUR 479 | |
 Anti- S100B Antibody |
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| A1659-100 | Biovision | EUR 479 | |
 Anti-SLUG Antibody |
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| A1660-100 | Biovision | EUR 370 | |
 Anti-SOX1 Antibody |
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| A1661-100 | Biovision | EUR 479 | |
 Anti-SOX11 Antibody |
|||
| A1662-100 | Biovision | EUR 370 | |
 Anti-EOMES Antibody |
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| A1665-100 | Biovision | EUR 370 | |
 Anti-TGIF1 Antibody |
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| A1667-100 | Biovision | EUR 479 | |
 Anti-CD9 Antibody |
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| A1668-100 | Biovision | EUR 370 | |
 Anti-CD168 Antibody |
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| A1671-100 | Biovision | EUR 479 | |
 Anti-TF2L1 Antibody |
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| A1673-100 | Biovision | EUR 479 | |
 Anti-DDIT3 Antibody |
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| A1674-100 | Biovision | EUR 370 | |
 Anti-DDX3 Antibody |
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| A1675-100 | Biovision | EUR 479 | |
 Anti-EHMT2 Antibody |
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| A1676-100 | Biovision | EUR 479 | |
 Anti-ERAS Antibody |
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| A1677-100 | Biovision | EUR 479 | |
 Anti-FOXD3 Antibody |
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| A1678-100 | Biovision | EUR 479 | |
 Anti-GREM2 Antibody |
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| A1679-100 | Biovision | EUR 479 | |
 Anti-HOXB8 Antibody |
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| A1680-100 | Biovision | EUR 479 | |
 Anti-ITGA6 Antibody |
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| A1681-100 | Biovision | EUR 479 | |
 Anti-JMJD6 Antibody |
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| A1683-100 | Biovision | EUR 370 | |
 Anti-LIN28B Antibody |
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| A1685-100 | Biovision | EUR 479 | |
 Anti-MELK Antibody |
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| A1686-100 | Biovision | EUR 370 | |
 Anti-NODAL Antibody |
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| A1687-100 | Biovision | EUR 370 | |
 Anti-LRH1 Antibody |
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| A1688-100 | Biovision | EUR 370 | |
 Anti-Nucleostemin Antibody |
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| A1689-100 | Biovision | EUR 370 | |
 Anti-PUM2 Antibody |
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| A1690-100 | Biovision | EUR 479 | |
 Anti-Sall4 Antibody |
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| A1692-100 | Biovision | EUR 479 | |
 Anti-SGK1 Antibody |
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| A1693-100 | Biovision | EUR 479 | |
 Anti-SHB Antibody |
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| A1694-100 | Biovision | EUR 479 | |
 Anti-SHH Antibody |
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| A1695-100 | Biovision | EUR 479 | |
 Anti-ALB Antibody |
|||
| A1696-100 | Biovision | EUR 479 | |
 Anti-Fibronectin Antibody |
|||
| A1697-100 | Biovision | EUR 479 | |
 Anti-PKM Antibody |
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| A1698-100 | Biovision | EUR 479 | |
 Anti-TFF3 Antibody |
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| A1699-100 | Biovision | EUR 479 | |
 Anti-EPHX1 Antibody |
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| A1700-100 | Biovision | EUR 479 | |
 Anti-UPK3B Antibody |
|||
| A1701-100 | Biovision | EUR 479 | |
 Anti-Ubiquitin Antibody |
|||
| A1702-100 | Biovision | EUR 479 | |
 Anti-PCP4 Antibody |
|||
| A1703-100 | Biovision | EUR 479 | |
 Anti-S100A4 Antibody |
|||
| A1704-100 | Biovision | EUR 479 | |
 Anti-MAGEA4 Antibody |
|||
| A1705-100 | Biovision | EUR 479 | |
 Anti-RAGE Antibody |
|||
| A1706-100 | Biovision | EUR 479 | |
 Anti-Fibrinogen Antibody |
|||
| A1708-100 | Biovision | EUR 370 | |
Anti-Fibrinogen Antibody |
|||
| A1708-30T | Biovision | EUR 146 | |
 Anti-Enterokinase Antibody |
|||
| A1710-100 | Biovision | EUR 349 | |
Anti-Enterokinase Antibody |
|||
| A1710-30T | Biovision | EUR 146 | |
 Anti-PPDK Antibody |
|||
| A1711-100 | Biovision | EUR 349 | |
Anti-PPDK Antibody |
|||
| A1711-30T | Biovision | EUR 146 | |
 Anti-CD66e Antibody |
|||
| A1739-100 | Biovision | EUR 479 | |
 Anti-ADAP1 Antibody |
|||
| A1740-100 | Biovision | EUR 479 | |
 Anti-UTS2 Antibody |
|||
| A1741-100 | Biovision | EUR 479 | |
 Anti-ACOT2 Antibody |
|||
| A1742-100 | Biovision | EUR 479 | |
 Anti-ACVR1B Antibody |
|||
| A1743-100 | Biovision | EUR 479 | |
 Anti-ADD1 Antibody |
|||
| A1744-100 | Biovision | EUR 479 | |
 Anti-AKR1B1 Antibody |
|||
| A1746-100 | Biovision | EUR 479 | |
 Anti-ALOX5 Antibody |
|||
| A1747-100 | Biovision | EUR 479 | |
 Anti-APLP1 Antibody |
|||
| A1749-100 | Biovision | EUR 479 | |
 Anti-ANXA7 Antibody |
|||
| A1750-100 | Biovision | EUR 479 | |
 Anti-Taq Antibody |
|||
| G930 | ABM | 250 U (50 ul) | EUR 139 |
 Anti-Isoniazid Antibody |
|||
| A1971-30 | Biovision | 30 µg | EUR 146 |
 Anti-UNR Antibody |
|||
| A1988-100 | Biovision | 100 µl | EUR 384 |
Gemultiplexte en high-throughput labelvrije detectie van RNA/Spike Protein /IgG/IgM-biomarkers van SARS-CoV-2-infectie met behulp van nanoplasmonische biosensoren
Om de COVID-19-uitbraak, die wordt veroorzaakt door het ernstige acute respiratoire syndroom coronavirus 2 (SARS-CoV-2), aan te pakken, is er een onvervulde behoefte aan zeer nauwkeurige diagnostische checks in alle stadia van infectie met snelle resultaten en hoge specificiteit. Hier presenteren we een labelvrije, op nanoplasmonische biosensor gebaseerde, multiplex screeningtest voor COVID-19 die 10 verschillende biomarkers kwantitatief kan detecteren (6 virale nucleïnezuurgenen, 2 spike-eiwitsubeenheden en 2 antilichamen) met een detectielimiet in de aM-reeks, allemaal binnen één biosensorplatform.
Onze nieuw ontwikkelde nanoplasmonische biosensoren vertonen een hoge specificiteit, wat van het grootste belang is om valse reacties te voorkomen. Als proof of idea laten we zien dat onze detectiebenadering het potentieel heeft om zowel IgG- als IgM- antilichamen rechtstreeks uit COVID-19-positieve patiëntplasmamonsters te kwantificeren in een enkele instrumentrun, wat de hoge doorvoercapaciteit van onze detectiebenadering aantoont. Het belangrijkste is dat onze take a look at ontvangende operationele kenmerken biedt, gebieden onder de curve van respectievelijk 0,997 en 0,999 voor IgG en IgM. De berekende p- waarde bepaald met de Mann-Whitney niet-parametrische take a look at is <0,0001 voor beide antilichamen wanneer de take a look at van COVID-19-positieve patiënten ( n = 80) wordt vergeleken met die van gezonde personen ( n = 72).
Bovendien geeft de screeningstest een berekende sensitiviteit (true constructive fee) van 100% (80/80), een specificiteit (true negatieve fee) >96% (77/80) , een positief voorspellende waarde van 98% bij een prevalentie van 5% en een negatief voorspellende waarde van 100% bij een prevalentie van 5%. Wij zijn van mening dat onze zeer gevoelige, multiplex, high-throughput testbenadering potentiële toepassingen heeft in de COVID-19-diagnostiek , met title bij het bepalen van virusprogressie en infectie-ernst voor clinici voor een geschikte behandeling, en ook een zeer effectieve diagnostische take a look at zal blijken te zijn wanneer toegepast op ziekten buiten de COVID-19-pandemie.
Nauwkeurigheid van alternatieve niet-polariseerbare krachtvelden voor de bepaling van eiwit- ligandbindingsaffiniteiten gedomineerd door kation-π-interacties
Het aanpassen van paarspecifieke Lennard-Jones-parameters by way of de niet-gebonden FIX (NBFIX)-functie van het CHARMM36-krachtveld is kosteneffectief gebleken voor het verbeteren van de beschrijving van kation-π-interacties in biologische objecten door middel van paarsgewijs additieve potentiële energiefuncties. Hier worden twee units nieuw geoptimaliseerde CHARMM36-krachtveldparameters, waaronder NBFIX-correcties, bedacht CHARMM36m-NBF en CHARMM36-WYF, en de originele krachtvelden, namelijk CHARMM36m en Amber ff14SB, gebruikt om de standaard bindingsvrije energieën van zeven eiwit- ligandcomplexen die kation-π-interacties bevatten.
Vergeleken met nauwkeurige experimentele metingen, geven onze resultaten aan dat de ongecorrigeerde, originele krachtvelden de bindingsvrije energieën vital onderschatten, met een gemiddelde fout van 5,Three kcal/mol, terwijl de gemiddelde fouten van CHARMM36m-NBF en CHARMM36-WYF 0,eight en 2,1 bedragen. respectievelijk kcal/mol. De huidige studie toont overtuigend aan dat het gebruik van gewijzigde parameters samen met NBFIX-correcties de nauwkeurigheid van de standaard bindingsvrije energie van eiwit-ligandcomplexen die worden gedomineerd door kation-π-interacties, met title met CHARMM36m-NBF, dramatisch verhoogt.
Gebruikmakend van door tijd opgeloste, door proteïne geïnduceerde fluorescentieverbetering om stabiele lokale conformaties te identificeren, één α-synucleïne-monomeer per keer
Het gebruik van spectroscopische linialen om meerdere conformaties van afzonderlijke biomoleculen en hun dynamiek te volgen, heeft een revolutie teweeggebracht in het begrip van structurele dynamiek en zijn bijdragen aan de biologie. Terwijl de op FRET gebaseerde liniaal rapporteert over afstanden tussen kleurstoffen in het bereik van 3-10 nm, rapporteren andere spectroscopische technieken, zoals eiwit-geïnduceerde fluorescentieverbetering (PIFE), over de nabijheid tussen een kleurstof en een eiwitoppervlak in de kortere 0 -Three nm bereik. Ongeacht de gekozen methode, het gebruik ervan bij het één voor één meten van vrij diffunderende biomoleculen haalt histogrammen op van de experimentele parameter die afzonderlijke centraal verdeelde subpopulaties van biomoleculen oplevert, waarbij elke subpopulatie vertegenwoordigtofwel een enkele conformatie die binnen milliseconden onveranderd bleef, of meerdere conformaties die veel sneller dan milliseconden in elkaar overgaan, en dus een uitgemiddelde subpopulatie.
In FRET met één molecuul, waar de gerapporteerde parameter in histogrammen de FRET-efficiëntie tussen kleurstoffen is, werd eerder gerapporteerd dat een intrinsiek ongeordend eiwit, zoals het α-synucleïne-monomeer in buffer, een enkele gemiddelde subpopulatie van meerdere conformaties die snel in elkaar overgaan. Hoewel deze eerdere bevindingen afhankelijk zijn van het bereik van 3-10 nm van de FRET-gebaseerde liniaal, hebben we geprobeerd dit eiwit op de proef te stellen met behulp van PIFE met één molecuul, waarbij we de fluorescentielevensduur van plaatsspecifieke sCy3-gelabelde α-Synuclein volgen. eiwitten één voor één. Interessant is dat met behulp van deze spectroscopische naderingssensor met een kortere afstand, sCy3-gelabeld -Synucleïne verschillende subpopulaties met een duidelijk verschillende gemiddelde levensduur vertoont die in 10-100 ms in elkaar overgaan.Deze resultaten laten zien dat, hoewel α-synucleïne wereldwijd ontregeld kan zijn, het toch stabiele lokale structuren bereikt. Samenvattend, in dit werk benadrukken we het voordeel van het gebruik van verschillende spectroscopische nabijheidssensoren die lokale of globale structurele veranderingen één biomolecuul tegelijk volgen.