SPC1 regelt de signaal-peptidase bemiddelde verwerken van membraan proteïnen
Signaalpeptidase (SPase) splitst de signaalsequenties (SS’s ) van secretoire voorlopers. Het bevat een evolutionair geconserveerde membraaneiwitsubeenheid, Spcl, die niet nodig is voor de katalytische activiteit van SPase, en de rol ervan blijft onbekend. In de huidige studie hebben we de functie van gist Spc1 onderzocht. Eerst hebben we een in vivo SPase-splitsingstest opgezet met behulp van secretoire eiwit-CPY- varianten met SS’s gemodificeerd in de n- en h-regio’s.
Bij het vergelijken van de SS-splitsingsefficiëntie van deze varianten in cellen met of zonder Spcl, ontdekten we dat signaalverankerde sequenties vatbaarder werden voor splitsing door SPase zonder Spcl. Verder was SPase-gemedieerde verwerking van modelmembraaneiwitten verbeterd in de afwezigheid van maar verminderd bij overexpressie van Spcl. Spcl werd geco-immunoprecipiteerd met eiwitten die niet-gesplitste signaalverankerde of transmembraan (TM) segmenten droegen.
Samengevat suggereren deze resultaten dat Spc1 TM-segmenten beschermt tegen SPase-actie, waardoor de substraatselectie voor SPase wordt verscherpt en fungeert als een negatieve regulator voor de SPase-gemedieerde verwerking van membraaneiwitten .
Interleukine-10 speelt een belangrijke rol in pasgeboren ratten met Hypoxic-ischemie geassocieerd met B-cel lymfoom 2 en endoplasmatisch reticulum Protein 29
Interleukine 10 (IL-10) is een synthetische remmer van humane cytokines met immunomodulerende en ontstekingsremmende effecten. Deze studie was bedoeld om de expressievariatie van IL-10 op meerdere plaatsen, waaronder cortex, hippocampus en longweefsels van neonatale hypoxisch-ischemische (HI) ratten te onderzoeken en de cruciale rol van IL-10 bij het verlichten van HI-hersenschade te onderzoeken. In deze studie werden neonatale Sprague-Dawley-ratten onderworpen aan de ligatie van de rechter gemeenschappelijke halsslagader, gevolgd door 2 uur hypoxie.
De expressie van IL-10 in de cortex, hippocampus en longweefsels werd gemeten met immunohistochemie, realtime kwantitatieve polymerasekettingreactie (RT-qPCR) en western blot (WB). Dubbele kleuring met immunofluorescentie werd uitgevoerd om de lokalisatie van IL-10 in neuronen en astrocyten te observeren . Bovendien werden niet-targeting en concentrating on IL-10 siRNA-lentivirusvectoren geïnjecteerd in respectievelijk de ratten van de negatieve controle (NC) en IL-10-groep, en de mRNA-niveaus van B-cellymfoom 2 (Bcl-2) en endoplasmatische reticulum eiwit 29 (ERp29) werden gedetecteerd door RT-qPCR na IL-10 stilte.
De resultaten toonden aan dat de IL-10-expressie aanzienlijk was verhoogd nadat HI en IL-10 waren gecolokaliseerd met neuronen en astrocyten die ernstig waren beschadigd door HI-belediging. Bovendien waren Bcl-2 en ERp29 opmerkelijk verlaagd na IL-10-mRNA-interferentie in vergelijking met de NC-groep. Onze bevindingen onthulden dat IL-10 zijn anti-apoptotische en neuroprotectieve effecten uitoefende door de expressie van Bcl-2 en ERp29 te reguleren, wat aangeeft dat IL-10 een veelbelovend molecuuldoelwit kan zijn voor HIE-behandeling.

GCFC2 Blocking Peptide |
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20-abx063333 | Abbexa |
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Human GCFC2 shRNA Plasmid |
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20-abx954755 | Abbexa |
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Mouse GCFC2 shRNA Plasmid |
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20-abx983608 | Abbexa |
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GCFC2 Recombinant Protein (Rat) |
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RP202352 | ABM | 100 ug | Ask for price |
GCFC2 Recombinant Protein (Human) |
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RP060264 | ABM | 100 ug | Ask for price |
Gcfc2 ORF Vector (Rat) (pORF) |
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ORF067452 | ABM | 1.0 ug DNA | EUR 607.2 |
GCFC2 ORF Vector (Human) (pORF) |
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ORF020089 | ABM | 1.0 ug DNA | Ask for price |
Human GCFC2 Protein Lysate 20ug |
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IHUGCFC2PLLY20UG | Innovative research | each | EUR 213 |
Description: Human GCFC2 Protein Lysate 20ug |
GCFC2 Protein Vector (Rat) (pPM-C-HA) |
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PV269808 | ABM | 500 ng | EUR 1399.2 |
GCFC2 Protein Vector (Rat) (pPB-C-His) |
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PV269806 | ABM | 500 ng | EUR 1399.2 |
GCFC2 Protein Vector (Rat) (pPB-N-His) |
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PV269807 | ABM | 500 ng | EUR 1399.2 |
GCFC2 Protein Vector (Rat) (pPM-C-His) |
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PV269809 | ABM | 500 ng | EUR 1399.2 |
GCFC2 Protein Vector (Human) (pPM-C-HA) |
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PV080355 | ABM | 500 ng | Ask for price |
GCFC2 Protein Vector (Human) (pPB-C-His) |
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PV080353 | ABM | 500 ng | Ask for price |
GCFC2 Protein Vector (Human) (pPB-N-His) |
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PV080354 | ABM | 500 ng | Ask for price |
GCFC2 Protein Vector (Human) (pPM-C-His) |
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PV080356 | ABM | 500 ng | Ask for price |
GCFC1 Antibody |
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45987-100ul | SAB | 100ul | EUR 302.4 |
GCFC1 Antibody |
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45987-50ul | SAB | 50ul | EUR 224.4 |
GCFC1 Antibody |
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ABD9529 | Lifescience Market | 100 ug | EUR 525.6 |
GCFC1 Antibody |
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DF9529-100ul | Affinity Biosciences | 100ul | EUR 280 |
GCFC1 Antibody |
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DF9529-200ul | Affinity Biosciences | 200ul | EUR 350 |
GCFC1 Antibody |
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DF9529 | Affinity Biosciences | 100ul | EUR 280 |
Description: Human,Mouse |
GCFC1 Antibody |
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E19-9529-1 | EnoGene | 50ug/50ul | EUR 145 |
Description: Available in various conjugation types. |
GCFC1 Antibody |
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E19-9529-2 | EnoGene | 100ug/100ul | EUR 225 |
Description: Available in various conjugation types. |
GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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LV654355 | ABM | 1.0 ug DNA | EUR 1626 |
GCFC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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LV654359 | ABM | 1.0 ug DNA | EUR 1626 |
GCFC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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LV654360 | ABM | 1.0 ug DNA | EUR 1626 |
GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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LV762095 | ABM | 1.0 ug DNA | EUR 1104 |
GCFC2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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LV762099 | ABM | 1.0 ug DNA | EUR 1104 |
GCFC2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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LV762100 | ABM | 1.0 ug DNA | EUR 1104 |
Human Chromosome 2 Open Reading Frame 3 (GCFC2) ELISA Kit |
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abx386199-96tests | Abbexa | 96 tests | EUR 1093.2 |
GCFC1 Conjugated Antibody |
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C45987 | SAB | 100ul | EUR 476.4 |
GCFC1 Polyclonal Antibody |
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ABP58618-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of GCFC1 from Human, Mouse. This GCFC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GCFC1 protein |
GCFC1 Polyclonal Antibody |
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ABP58618-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of GCFC1 from Human, Mouse. This GCFC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GCFC1 protein |
GCFC1 Polyclonal Antibody |
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ABP58618-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of GCFC1 from Human, Mouse. This GCFC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GCFC1 protein |
GCFC1 Polyclonal Antibody |
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ES9671-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against GCFC1 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
GCFC1 Polyclonal Antibody |
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ES9671-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against GCFC1 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
GCFC1 Polyclonal Antibody |
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E11-200725 | EnoGene | 100ug/100ul | EUR 225 |
Description: Available in various conjugation types. |
GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV654356 | ABM | 1.0 ug DNA | EUR 1626 |
GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV654357 | ABM | 1.0 ug DNA | EUR 1695.6 |
GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV654358 | ABM | 1.0 ug DNA | EUR 1695.6 |
GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV762096 | ABM | 1.0 ug DNA | EUR 1104 |
GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV762097 | ABM | 1.0 ug DNA | EUR 1173.6 |
GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV762098 | ABM | 1.0 ug DNA | EUR 1173.6 |
GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody |
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20-abx217653 | Abbexa |
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Rabbit anti-GCFC2 Antibody |
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YLD3352-100ul | Shanghai YL Biotech | 100 ul | EUR 320 |
Description: Rabbit polyclonal antibody to GCFC2 |
Rabbit anti-GCFC2 Antibody |
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YLD3352-50ul | Shanghai YL Biotech | 50 ul | EUR 200 |
Description: Rabbit polyclonal antibody to GCFC2 |
Rabbit anti-GCFC2 Antibody |
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DL91139A-100ul | DL Develop | 100 ul | EUR 299 |
Description: C2orf3; GCF; TCF9; GC-rich sequence DNA-binding factor 2; GC-rich sequence DNA-binding factor; Transcription factor 9; TCF-9 |
Rabbit anti-GCFC2 Antibody |
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DL91139A-50ul | DL Develop | 50 ul | EUR 209.3 |
Description: C2orf3; GCF; TCF9; GC-rich sequence DNA-binding factor 2; GC-rich sequence DNA-binding factor; Transcription factor 9; TCF-9 |
GCFC1 ORF Vector (Human) (pORF) |
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ORF020087 | ABM | 1.0 ug DNA | Ask for price |
Gcfc1 ORF Vector (Mouse) (pORF) |
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ORF045375 | ABM | 1.0 ug DNA | EUR 607.2 |
Gcfc1 3'UTR GFP Stable Cell Line |
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TU156964 | ABM | 1.0 ml | Ask for price |
GCFC1 3'UTR GFP Stable Cell Line |
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TU058672 | ABM | 1.0 ml | EUR 2799.6 |
GCFC1 Blocking Peptide |
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DF9529-BP | Affbiotech | 1mg | EUR 234 |
GCFC1-AS1 ORF Vector (Human) (pORF) |
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ORF020088 | ABM | 1.0 ug DNA | Ask for price |
GCFC1 Protein Vector (Mouse) (pPM-C-HA) |
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PV181500 | ABM | 500 ng | EUR 1278 |
GCFC1 Protein Vector (Human) (pPM-C-HA) |
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PV080347 | ABM | 500 ng | Ask for price |
GCFC1 Protein Vector (Mouse) (pPB-C-His) |
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PV181498 | ABM | 500 ng | EUR 1278 |
GCFC1 Protein Vector (Mouse) (pPB-N-His) |
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PV181499 | ABM | 500 ng | EUR 1278 |
GCFC1 Protein Vector (Mouse) (pPM-C-His) |
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PV181501 | ABM | 500 ng | EUR 1278 |
GCFC1 Protein Vector (Human) (pPB-C-His) |
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PV080345 | ABM | 500 ng | Ask for price |
GCFC1 Protein Vector (Human) (pPB-N-His) |
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PV080346 | ABM | 500 ng | Ask for price |
GCFC1 Protein Vector (Human) (pPM-C-His) |
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PV080348 | ABM | 500 ng | Ask for price |
GCFC1 3'UTR Luciferase Stable Cell Line |
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TU008672 | ABM | 1.0 ml | EUR 2799.6 |
Gcfc1 3'UTR Luciferase Stable Cell Line |
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TU106964 | ABM | 1.0 ml | Ask for price |
Gcfc1 sgRNA CRISPR Lentivector set (Mouse) |
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K3520201 | ABM | 3 x 1.0 ug | EUR 406.8 |
GCFC1 sgRNA CRISPR Lentivector set (Human) |
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K2771901 | ABM | 3 x 1.0 ug | EUR 406.8 |
Gcfc1 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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K3520202 | ABM | 1.0 ug DNA | EUR 184.8 |
Gcfc1 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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K3520203 | ABM | 1.0 ug DNA | EUR 184.8 |
Gcfc1 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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K3520204 | ABM | 1.0 ug DNA | EUR 184.8 |
GCFC1 sgRNA CRISPR Lentivector (Human) (Target 1) |
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K2771902 | ABM | 1.0 ug DNA | EUR 184.8 |
GCFC1 sgRNA CRISPR Lentivector (Human) (Target 2) |
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K2771903 | ABM | 1.0 ug DNA | EUR 184.8 |
GCFC1 sgRNA CRISPR Lentivector (Human) (Target 3) |
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K2771904 | ABM | 1.0 ug DNA | EUR 184.8 |
Mouse GC- rich sequence DNA- binding factor 1, Gcfc1 ELISA KIT |
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ELI-37633m | Lifescience Market | 96 Tests | EUR 1038 |
Human GC- rich sequence DNA- binding factor 1, GCFC1 ELISA KIT |
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ELI-08155h | Lifescience Market | 96 Tests | EUR 988.8 |
Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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K3520205 | ABM | 3 x 1.0 ug | EUR 451.2 |
GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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K2771905 | ABM | 3 x 1.0 ug | EUR 451.2 |
GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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LV726545 | ABM | 1.0 ug DNA | Ask for price |
GCFC1-AS1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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LV726549 | ABM | 1.0 ug DNA | Ask for price |
Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1) |
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K3520206 | ABM | 1.0 ug DNA | EUR 200.4 |
Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2) |
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K3520207 | ABM | 1.0 ug DNA | EUR 200.4 |
Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3) |
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K3520208 | ABM | 1.0 ug DNA | EUR 200.4 |
GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) |
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K2771906 | ABM | 1.0 ug DNA | EUR 200.4 |
GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2) |
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K2771907 | ABM | 1.0 ug DNA | EUR 200.4 |
GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) |
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K2771908 | ABM | 1.0 ug DNA | EUR 200.4 |
GCFC1-AS1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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LV726550 | ABM | 1.0 ug DNA | Ask for price |
GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV726546 | ABM | 1.0 ug DNA | Ask for price |
GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV726547 | ABM | 1.0 ug DNA | Ask for price |
GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV726548 | ABM | 1.0 ug DNA | Ask for price |
Anti-Mouse C3 Antibody | GC3-90F-Z |
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GC3-90F-Z | Immunology Consultants Laboratory | 0.1 mg | EUR 316 |
Description: Anti-Mouse C3 Antibody ; GC3-90F-Z ; Immunology Consultants Laboratory Host: Goat Format: FITC Conjugated Product Type: Primary Antibody Antibody Clonality: Polyclonal |
pCMV-SPORT6-GCFC2-G181A |
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PVT12797 | Lifescience Market | 2 ug | EUR 469.2 |
GCF antibody |
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70R-36476 | Fitzgerald | 100 ug | EUR 392.4 |
Description: Rabbit polyclonal GCF antibody |
GCF Antibody |
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34702-100ul | SAB | 100ul | EUR 302.4 |
GCF Antibody |
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34702-50ul | SAB | 50ul | EUR 224.4 |
GCF Antibody |
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ABD4083 | Lifescience Market | 100 ug | EUR 525.6 |
GCF Antibody |
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20-abx014492 | Abbexa |
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GCF Antibody |
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DF4083 | Affinity Biosciences | 100ul | EUR 280 |
Description: Human,Mouse |
GCF Antibody |
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E034702 | EnoGene | 100μg/100μl | EUR 255 |
Description: Available in various conjugation types. |
GCF Antibody |
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E19-4083 | EnoGene | 100μg/100μl | EUR 225 |
Description: Available in various conjugation types. |
GCF Antibody |
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E11-15891C | EnoGene | 100μg | EUR 225 |
Description: Available in various conjugation types. |
GCF Antibody |
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DF4083-100ul | Affinity Biosciences | 100ul | EUR 280 |
GCF Antibody |
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DF4083-200ul | Affinity Biosciences | 200ul | EUR 350 |
GCF Conjugated Antibody |
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C34702 | SAB | 100ul | EUR 476.4 |
Anti-Anti-SEPT5 Antibody antibody |
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STJ114819 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
Anti-Anti-SEPT2 Antibody antibody |
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STJ28365 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT7 Antibody antibody |
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STJ28963 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
Anti-Anti-SEPT3 Antibody antibody |
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STJ118990 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT1 antibody antibody |
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STJ119580 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012] |
Anti-Anti-SEPT6 antibody antibody |
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STJ11100949 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. |
Anti-Anti-SEPT9 Antibody antibody |
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STJ111369 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described. |
Anti-Anti-SEPT4 Antibody antibody |
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STJ112276 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. |
Anti-Anti-SEPT7 Antibody antibody |
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STJ116214 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
Anti-Anti-SEPT8 Antibody antibody |
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STJ117206 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-SEPT2 Antibody antibody |
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STJ25475 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT5 Antibody antibody |
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STJ25477 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
Anti-Anti-SEPT8 Antibody antibody |
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STJ25479 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-MARCH9 Antibody antibody |
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STJ112609 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT11 Antibody antibody |
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STJ113941 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT11 Antibody antibody |
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STJ114081 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-MARCH8 Antibody antibody |
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STJ114828 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT12 Antibody antibody |
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STJ117759 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-MARCH6 Antibody antibody |
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STJ118549 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-MARCH6 Antibody antibody |
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STJ118550 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-MARCH7 Antibody antibody |
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STJ118752 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT11 Antibody antibody |
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STJ111530 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-DDB1 Antibody |
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A00333 | BosterBio | 100uL | EUR 546 |
Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse. |
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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AEA465Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6777.36 |
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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AEA465Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 663.31 |
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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AEA465Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 896.16 |
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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AEA465Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3672.72 |
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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4-AEA465Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. |
Human Anti-AsAb(Anti-Anti-Sperm Antibody Antibody) ELISA Kit |
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ELK8071-48T | ELK Biotech | 48T | Ask for price |
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-AsAb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AsAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AsAb in the samples is then determined by comparing the OD of the samples to the standard curve. |
Human Anti-AsAb(Anti-Anti-Sperm Antibody Antibody) ELISA Kit |
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ELK8071-96T | ELK Biotech | 96T | Ask for price |
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-AsAb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AsAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AsAb in the samples is then determined by comparing the OD of the samples to the standard curve. |
Anti-BOD1 antibody |
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STJ72155 | St John's Laboratory | 100 µg | EUR 430.8 |
Anti-BOD1L antibody |
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STJ73530 | St John's Laboratory | 100 µg | EUR 312 |
ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody) |
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ELK8071 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
anti- Antibody^Polyclonal antibody control antibody |
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LSMab09882 | Lifescience Market | 100 ug | EUR 525.6 |
Anti-2B4 Antibody |
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HA500180 | HUABIO | 100ul | EUR 189 |
Description: This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Anti-2B4 Antibody |
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HA500263 | HUABIO | 100ul | EUR 189 |
Description: Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor . Activating function implicates association with SH2D1A and FYN . Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2. Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis . Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells. Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1. At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells. Involved in the regulation of CD8+ T-cell proliferation; expression on activated T-cells and binding to CD488 provides costimulatory-like function for neighboring T-cells. Inhibits inflammatory responses in dendritic cells (DCs) . |
Anti-7B2 antibody |
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STJ191062 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to 7B2 |
Anti-4.1G Antibody |
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A03718 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for 4.1G Antibody (EPB41L2) detection.tested for WB in Human. |
Anti-4.1G antibody |
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STJ91381 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to 4.1G. |
Anti-4.1R antibody |
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STJ91382 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to 4.1R. |
Anti-BOC Antibody |
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PA1904 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-BOK antibody |
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STJ190601 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to BOK |
Anti-BOC antibody |
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STJ71520 | St John's Laboratory | 100 µg | EUR 430.8 |
Anti-BOK antibody |
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STJ72368 | St John's Laboratory | 100 µg | EUR 430.8 |
Anti-BOC antibody |
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STJ192335 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to BOC |
Anti-BOC antibody |
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STJ29254 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: The protein encoded by this gene is a member of the immunoglobulin/fibronectin type III repeat family. It is a component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells, and promotes myogenic differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Anti-BoV antibody |
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STJ400075 | St John's Laboratory | 1 mg | EUR 535.2 |
Description: Human bocavirus (also termed HBoV) is a small non-enveloped virus with a single- stranded DNA genome. It is a member of the Parvoviridae family. HBoV has been detected worldwide in 2–20 % of all upper and lower respiratory tract infections, and has also been linked to gastroenteritis. |
Anti-BOK antibody |
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STJ118794 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-BOC antibody |
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STJ115407 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: The protein encoded by this gene is a member of the immunoglobulin/fibronectin type III repeat family. It is a component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells, and promotes myogenic differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Anti-2B1F antibody |
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STJ190870 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to 2B1F |
Anti-53BP1 antibody |
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STJ91389 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to 53BP1. |
Anti-53BP1 antibody |
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STJ91390 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to 53BP1. |
Anti-53BP1 antibody |
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STJ91391 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to 53BP1. |
Anti-BOB1 Antibody |
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ER1803-97 | HUABIO | 100ul | EUR 189 |
Description: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Reduced activation of Bob1-deficient B cells following antigenic stimulation is often reported. The action of Bob1 on B cell maturation and tolerance could be mediated by effects on B cell activation, in part by regulating the expression of immunosuppressive miRNAs. Bob1 has long been considered a B cell‐specific factor that interacts with the transcription factors Oct1 and Oct2 to enhance octamer‐dependent transcription. Bob1 can directly bind to and transactivate the promoters of Bcl6 and Btla. |
Anti-BOB1 antibody |
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STJ180315 | St John's Laboratory | 0.1 ml | EUR 254.4 |
Anti-BORA antibody |
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STJ190128 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to BORA |
Anti-BOP1 antibody |
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STJ191341 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to BOP1 |
Anti-BOCT antibody |
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STJ96427 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to BOCT. |
Anti-BORA antibody |
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STJ115786 | St John's Laboratory | 100 µl | EUR 332.4 |
anti- TH antibody |
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FNab08655 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against TH |
anti- XK antibody |
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FNab09543 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against XK |
anti- PR antibody |
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FNab09774 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against PR |
anti- T3 antibody |
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FNab09808 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against T3 |
anti- T4 antibody |
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FNab09809 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against T4 |
anti- TH antibody |
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FNab09870 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against TH |
anti- C9 antibody |
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FNab10107 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against C9 |
anti- C9 antibody |
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FNab10108 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against C9 |
anti- Rb antibody |
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FNab07140 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Rb |
anti- ER antibody |
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FNab02822 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against ER |
anti- F2 antibody |
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FNab02920 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against F2 |
anti- F5 antibody |
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FNab02923 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against F5 |
anti- F7 antibody |
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FNab02924 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against F7 |
anti- F9 antibody |
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FNab02925 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against F9 |
anti- FH antibody |
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FNab03106 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against FH |
anti- FH antibody |
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FNab03107 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against FH |
anti- HP antibody |
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FNab03990 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against HP |
anti- C6 antibody |
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FNab01123 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against C6 |
anti- C7 antibody |
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FNab01132 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against C7 |
Anti-B23 antibody |
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PAab00759 | Lifescience Market | 100 ug | EUR 494.4 |
Anti-B19 Antibody |
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STJ501374 | St John's Laboratory | 100 µg | EUR 571.2 |
Anti-B23 antibody |
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STJ97858 | St John's Laboratory | 100 µl | EUR 280.8 |
Description: Mouse monoclonal to B23. |
Anti-B23 antibody |
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STJ98470 | St John's Laboratory | 100 µl | EUR 280.8 |
Description: Mouse monoclonal to B23. |
Anti-B23 antibody |
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STJ91794 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to B23. |
anti- B23 antibody |
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FNab00759 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against B23 |
anti- B23 antibody |
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FNab00760 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against B23 |
Anti-3BP1 antibody |
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STJ191408 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to 3BP1 |
Anti-3BP2 antibody |
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STJ191409 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to 3BP2 |
Gemultiplexte en high-throughput labelvrije detectie van RNA/Spike Protein /IgG/IgM-biomarkers van SARS-CoV-2-infectie met behulp van nanoplasmonische biosensoren
Om de COVID-19-uitbraak, die wordt veroorzaakt door het ernstige acute respiratoire syndroom coronavirus 2 (SARS-CoV-2), aan te pakken, is er een onvervulde behoefte aan zeer nauwkeurige diagnostische checks in alle stadia van infectie met snelle resultaten en hoge specificiteit. Hier presenteren we een labelvrije, op nanoplasmonische biosensor gebaseerde, multiplex screeningtest voor COVID-19 die 10 verschillende biomarkers kwantitatief kan detecteren (6 virale nucleïnezuurgenen, 2 spike-eiwitsubeenheden en 2 antilichamen) met een detectielimiet in de aM-reeks, allemaal binnen één biosensorplatform.
Onze nieuw ontwikkelde nanoplasmonische biosensoren vertonen een hoge specificiteit, wat van het grootste belang is om valse reacties te voorkomen. Als proof of idea laten we zien dat onze detectiebenadering het potentieel heeft om zowel IgG- als IgM- antilichamen rechtstreeks uit COVID-19-positieve patiëntplasmamonsters te kwantificeren in een enkele instrumentrun, wat de hoge doorvoercapaciteit van onze detectiebenadering aantoont. Het belangrijkste is dat onze take a look at ontvangende operationele kenmerken biedt, gebieden onder de curve van respectievelijk 0,997 en 0,999 voor IgG en IgM. De berekende p- waarde bepaald met de Mann-Whitney niet-parametrische take a look at is <0,0001 voor beide antilichamen wanneer de take a look at van COVID-19-positieve patiënten ( n = 80) wordt vergeleken met die van gezonde personen ( n = 72).
Bovendien geeft de screeningstest een berekende sensitiviteit (true constructive fee) van 100% (80/80), een specificiteit (true negatieve fee) >96% (77/80) , een positief voorspellende waarde van 98% bij een prevalentie van 5% en een negatief voorspellende waarde van 100% bij een prevalentie van 5%. Wij zijn van mening dat onze zeer gevoelige, multiplex, high-throughput testbenadering potentiële toepassingen heeft in de COVID-19-diagnostiek , met title bij het bepalen van virusprogressie en infectie-ernst voor clinici voor een geschikte behandeling, en ook een zeer effectieve diagnostische take a look at zal blijken te zijn wanneer toegepast op ziekten buiten de COVID-19-pandemie.
Nauwkeurigheid van alternatieve niet-polariseerbare krachtvelden voor de bepaling van eiwit- ligandbindingsaffiniteiten gedomineerd door kation-π-interacties
Het aanpassen van paarspecifieke Lennard-Jones-parameters by way of de niet-gebonden FIX (NBFIX)-functie van het CHARMM36-krachtveld is kosteneffectief gebleken voor het verbeteren van de beschrijving van kation-π-interacties in biologische objecten door middel van paarsgewijs additieve potentiële energiefuncties. Hier worden twee units nieuw geoptimaliseerde CHARMM36-krachtveldparameters, waaronder NBFIX-correcties, bedacht CHARMM36m-NBF en CHARMM36-WYF, en de originele krachtvelden, namelijk CHARMM36m en Amber ff14SB, gebruikt om de standaard bindingsvrije energieën van zeven eiwit- ligandcomplexen die kation-π-interacties bevatten.
Vergeleken met nauwkeurige experimentele metingen, geven onze resultaten aan dat de ongecorrigeerde, originele krachtvelden de bindingsvrije energieën vital onderschatten, met een gemiddelde fout van 5,Three kcal/mol, terwijl de gemiddelde fouten van CHARMM36m-NBF en CHARMM36-WYF 0,eight en 2,1 bedragen. respectievelijk kcal/mol. De huidige studie toont overtuigend aan dat het gebruik van gewijzigde parameters samen met NBFIX-correcties de nauwkeurigheid van de standaard bindingsvrije energie van eiwit-ligandcomplexen die worden gedomineerd door kation-π-interacties, met title met CHARMM36m-NBF, dramatisch verhoogt.
Gebruikmakend van door tijd opgeloste, door proteïne geïnduceerde fluorescentieverbetering om stabiele lokale conformaties te identificeren, één α-synucleïne-monomeer per keer
Het gebruik van spectroscopische linialen om meerdere conformaties van afzonderlijke biomoleculen en hun dynamiek te volgen, heeft een revolutie teweeggebracht in het begrip van structurele dynamiek en zijn bijdragen aan de biologie. Terwijl de op FRET gebaseerde liniaal rapporteert over afstanden tussen kleurstoffen in het bereik van 3-10 nm, rapporteren andere spectroscopische technieken, zoals eiwit-geïnduceerde fluorescentieverbetering (PIFE), over de nabijheid tussen een kleurstof en een eiwitoppervlak in de kortere 0 -Three nm bereik. Ongeacht de gekozen methode, het gebruik ervan bij het één voor één meten van vrij diffunderende biomoleculen haalt histogrammen op van de experimentele parameter die afzonderlijke centraal verdeelde subpopulaties van biomoleculen oplevert, waarbij elke subpopulatie vertegenwoordigtofwel een enkele conformatie die binnen milliseconden onveranderd bleef, of meerdere conformaties die veel sneller dan milliseconden in elkaar overgaan, en dus een uitgemiddelde subpopulatie.
In FRET met één molecuul, waar de gerapporteerde parameter in histogrammen de FRET-efficiëntie tussen kleurstoffen is, werd eerder gerapporteerd dat een intrinsiek ongeordend eiwit, zoals het α-synucleïne-monomeer in buffer, een enkele gemiddelde subpopulatie van meerdere conformaties die snel in elkaar overgaan. Hoewel deze eerdere bevindingen afhankelijk zijn van het bereik van 3-10 nm van de FRET-gebaseerde liniaal, hebben we geprobeerd dit eiwit op de proef te stellen met behulp van PIFE met één molecuul, waarbij we de fluorescentielevensduur van plaatsspecifieke sCy3-gelabelde α-Synuclein volgen. eiwitten één voor één. Interessant is dat met behulp van deze spectroscopische naderingssensor met een kortere afstand, sCy3-gelabeld -Synucleïne verschillende subpopulaties met een duidelijk verschillende gemiddelde levensduur vertoont die in 10-100 ms in elkaar overgaan.Deze resultaten laten zien dat, hoewel α-synucleïne wereldwijd ontregeld kan zijn, het toch stabiele lokale structuren bereikt. Samenvattend, in dit werk benadrukken we het voordeel van het gebruik van verschillende spectroscopische nabijheidssensoren die lokale of globale structurele veranderingen één biomolecuul tegelijk volgen.