SPC1 regelt de signaal-peptidase bemiddelde verwerken van membraan proteïnen
Signaalpeptidase (SPase) splitst de signaalsequenties (SS’s ) van secretoire voorlopers. Het bevat een evolutionair geconserveerde membraaneiwitsubeenheid, Spcl, die niet nodig is voor de katalytische activiteit van SPase, en de rol ervan blijft onbekend. In de huidige studie hebben we de functie van gist Spc1 onderzocht. Eerst hebben we een in vivo SPase-splitsingstest opgezet met behulp van secretoire eiwit-CPY- varianten met SS’s gemodificeerd in de n- en h-regio’s.
Bij het vergelijken van de SS-splitsingsefficiëntie van deze varianten in cellen met of zonder Spcl, ontdekten we dat signaalverankerde sequenties vatbaarder werden voor splitsing door SPase zonder Spcl. Verder was SPase-gemedieerde verwerking van modelmembraaneiwitten verbeterd in de afwezigheid van maar verminderd bij overexpressie van Spcl. Spcl werd geco-immunoprecipiteerd met eiwitten die niet-gesplitste signaalverankerde of transmembraan (TM) segmenten droegen.
Samengevat suggereren deze resultaten dat Spc1 TM-segmenten beschermt tegen SPase-actie, waardoor de substraatselectie voor SPase wordt verscherpt en fungeert als een negatieve regulator voor de SPase-gemedieerde verwerking van membraaneiwitten .
Interleukine-10 speelt een belangrijke rol in pasgeboren ratten met Hypoxic-ischemie geassocieerd met B-cel lymfoom 2 en endoplasmatisch reticulum Protein 29
Interleukine 10 (IL-10) is een synthetische remmer van humane cytokines met immunomodulerende en ontstekingsremmende effecten. Deze studie was bedoeld om de expressievariatie van IL-10 op meerdere plaatsen, waaronder cortex, hippocampus en longweefsels van neonatale hypoxisch-ischemische (HI) ratten te onderzoeken en de cruciale rol van IL-10 bij het verlichten van HI-hersenschade te onderzoeken. In deze studie werden neonatale Sprague-Dawley-ratten onderworpen aan de ligatie van de rechter gemeenschappelijke halsslagader, gevolgd door 2 uur hypoxie.
De expressie van IL-10 in de cortex, hippocampus en longweefsels werd gemeten met immunohistochemie, realtime kwantitatieve polymerasekettingreactie (RT-qPCR) en western blot (WB). Dubbele kleuring met immunofluorescentie werd uitgevoerd om de lokalisatie van IL-10 in neuronen en astrocyten te observeren . Bovendien werden niet-targeting en concentrating on IL-10 siRNA-lentivirusvectoren geïnjecteerd in respectievelijk de ratten van de negatieve controle (NC) en IL-10-groep, en de mRNA-niveaus van B-cellymfoom 2 (Bcl-2) en endoplasmatische reticulum eiwit 29 (ERp29) werden gedetecteerd door RT-qPCR na IL-10 stilte.
De resultaten toonden aan dat de IL-10-expressie aanzienlijk was verhoogd nadat HI en IL-10 waren gecolokaliseerd met neuronen en astrocyten die ernstig waren beschadigd door HI-belediging. Bovendien waren Bcl-2 en ERp29 opmerkelijk verlaagd na IL-10-mRNA-interferentie in vergelijking met de NC-groep. Onze bevindingen onthulden dat IL-10 zijn anti-apoptotische en neuroprotectieve effecten uitoefende door de expressie van Bcl-2 en ERp29 te reguleren, wat aangeeft dat IL-10 een veelbelovend molecuuldoelwit kan zijn voor HIE-behandeling.

GCFC2 Recombinant Protein (Rat) |
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RP202352 | ABM | 100 ug | Ask for price |
GCFC2 Recombinant Protein (Human) |
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RP060264 | ABM | 100 ug | Ask for price |
pCMV-SPORT6-GCFC2-G181A |
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PVT12797 | Lifescience Market | 2 ug | EUR 469.2 |
Polyclonal Goat anti-GST α-form |
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GST-ANTI-1 | Detroit R&D | 50 uL | EUR 336 |
Polyclonal Goat anti-GST μ-form |
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GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
Polyclonal Goat anti-GST p-form |
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GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
Gcfc2 ORF Vector (Rat) (pORF) |
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ORF067452 | ABM | 1.0 ug DNA | EUR 607.2 |
GCFC2 ORF Vector (Human) (pORF) |
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ORF020089 | ABM | 1.0 ug DNA | Ask for price |
Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody |
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20-abx008656 | Abbexa |
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Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody |
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abx330966-100ul | Abbexa | 100 ul | EUR 510 |
Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody |
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20-abx323787 | Abbexa |
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GCFC2 Protein Vector (Human) (pPB-C-His) |
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PV080353 | ABM | 500 ng | Ask for price |
GCFC2 Protein Vector (Human) (pPB-N-His) |
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PV080354 | ABM | 500 ng | Ask for price |
GCFC2 Protein Vector (Human) (pPM-C-HA) |
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PV080355 | ABM | 500 ng | Ask for price |
GCFC2 Protein Vector (Human) (pPM-C-His) |
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PV080356 | ABM | 500 ng | Ask for price |
GCFC2 Protein Vector (Rat) (pPB-C-His) |
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PV269806 | ABM | 500 ng | EUR 1399.2 |
GCFC2 Protein Vector (Rat) (pPB-N-His) |
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PV269807 | ABM | 500 ng | EUR 1399.2 |
GCFC2 Protein Vector (Rat) (pPM-C-HA) |
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PV269808 | ABM | 500 ng | EUR 1399.2 |
GCFC2 Protein Vector (Rat) (pPM-C-His) |
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PV269809 | ABM | 500 ng | EUR 1399.2 |
GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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LV654355 | ABM | 1.0 ug DNA | EUR 1626 |
GCFC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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LV654359 | ABM | 1.0 ug DNA | EUR 1626 |
GCFC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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LV654360 | ABM | 1.0 ug DNA | EUR 1626 |
GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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LV762095 | ABM | 1.0 ug DNA | EUR 1104 |
GCFC2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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LV762099 | ABM | 1.0 ug DNA | EUR 1104 |
GCFC2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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LV762100 | ABM | 1.0 ug DNA | EUR 1104 |
Human Chromosome 2 Open Reading Frame 3 (GCFC2) ELISA Kit |
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abx386199-96tests | Abbexa | 96 tests | EUR 1093.2 |
GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV654356 | ABM | 1.0 ug DNA | EUR 1626 |
GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV654357 | ABM | 1.0 ug DNA | EUR 1695.6 |
GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV654358 | ABM | 1.0 ug DNA | EUR 1695.6 |
GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV762096 | ABM | 1.0 ug DNA | EUR 1104 |
GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV762097 | ABM | 1.0 ug DNA | EUR 1173.6 |
GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV762098 | ABM | 1.0 ug DNA | EUR 1173.6 |
Positive control tissue section for each individua |
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Control-Slides-for-each-antibody | Innovex | Set of 25 | EUR 355 |
Description: Positive control tissue section for each individual antibody; Based on availability; INQUIRE |
Anti-Anti-SEPT6 antibody antibody |
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STJ11100949 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. |
Anti-Anti-SEPT9 Antibody antibody |
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STJ111369 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described. |
Anti-Anti-SEPT11 Antibody antibody |
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STJ111530 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT4 Antibody antibody |
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STJ112276 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. |
Anti-Anti-MARCH9 Antibody antibody |
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STJ112609 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT11 Antibody antibody |
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STJ113941 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT11 Antibody antibody |
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STJ114081 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT5 Antibody antibody |
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STJ114819 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
Anti-Anti-MARCH8 Antibody antibody |
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STJ114828 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT7 Antibody antibody |
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STJ116214 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
Anti-Anti-SEPT8 Antibody antibody |
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STJ117206 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-SEPT12 Antibody antibody |
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STJ117759 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-MARCH6 Antibody antibody |
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STJ118549 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-MARCH6 Antibody antibody |
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STJ118550 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-MARCH7 Antibody antibody |
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STJ118752 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT3 Antibody antibody |
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STJ118990 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT2 Antibody antibody |
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STJ28365 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT7 Antibody antibody |
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STJ28963 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
Anti-Anti-SEPT2 Antibody antibody |
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STJ25475 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT5 Antibody antibody |
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STJ25477 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
Anti-Anti-SEPT8 Antibody antibody |
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STJ25479 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Anti-Anti-SEPT1 antibody antibody |
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STJ119580 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012] |
Anti-Anti-DDB1 Antibody |
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A00333 | BosterBio | 100uL | EUR 546 |
Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse. |
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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AEA465Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6777.36 |
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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AEA465Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 663.31 |
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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AEA465Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 896.16 |
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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AEA465Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3672.72 |
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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4-AEA465Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. |
ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody) |
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ELK8071 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Anti-ZNF660 antibody |
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44742-100ul | SAB | 100ul | EUR 302.4 |
Anti-ZNF660 antibody |
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44742-50ul | SAB | 50ul | EUR 224.4 |
Anti-CCV Antibody |
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10-2789 | Fitzgerald | 1 mg | EUR 217.2 |
Description: Anti-Canine Corona Virus Antibody |
Anti-CCV Antibody |
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10-2790 | Fitzgerald | 1 mg | EUR 217.2 |
Description: Anti-Canine Corona Virus Antibody |
Anti-CDV antibody |
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10-2791 | Fitzgerald | 1 mg | EUR 198 |
Description: Anti-Canine Distemper Virus antibody |
Anti-CDV antibody |
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10-2792 | Fitzgerald | 1 mg | EUR 198 |
Description: Anti-Canine Distemper Virus antibody |
Anti-CDV antibody |
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10-2793 | Fitzgerald | 1 mg | EUR 198 |
Description: Anti-Canine Distemper Virus antibody |
Anti-CDV antibody |
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10-2794 | Fitzgerald | 1 mg | EUR 198 |
Description: Anti-Canine Distemper Virus antibody |
Anti-CPV Antibody |
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10-2795 | Fitzgerald | 500 uig | EUR 165.6 |
Description: Anti-Canine Parvo Virus Antibody |
Anti-CPV Antibody |
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10-2796 | Fitzgerald | 500 ug | EUR 165.6 |
Description: Anti-Canine Parvo Virus Antibody |
Anti-CPV Antibody |
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10-2797 | Fitzgerald | 500 ug | EUR 165.6 |
Description: Anti-Canine Parvo Virus Antibody |
Anti-C.CRP antibody |
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10-2798 | Fitzgerald | 500 ug | EUR 165.6 |
Description: Anti-Canine CRP antibody |
Anti-C.CRP antibody |
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10-2799 | Fitzgerald | 500 ug | EUR 165.6 |
Description: Anti-Canine CRP antibody |
Anti-CHW antibody |
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10-2800 | Fitzgerald | 1 mg | EUR 198 |
Description: Anti-Canine Heartworm antibody |
Anti-FeLV antibody |
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10-2801 | Fitzgerald | 1 mg | EUR 217.2 |
Description: Anti-Feline Leukemia Virus antibody |
Anti-FeLV antibody |
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10-2802 | Fitzgerald | 1 mg | EUR 217.2 |
Description: Anti-Feline Leukemia Virus antibody |
Anti-FeLV antibody |
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10-2803 | Fitzgerald | 1 mg | EUR 217.2 |
Description: Anti-Feline Leukemia Virus antibody |
Anti-PGI antibody |
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10-2806 | Fitzgerald | 1 mg | EUR 502.8 |
Description: Anti-PGI recombinant antibody |
Anti-PGI antibody |
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10-2807 | Fitzgerald | 1 mg | EUR 502.8 |
Description: Anti-PGI recombinant antibody |
Anti-PGII antibody |
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10-2808 | Fitzgerald | 1 mg | EUR 502.8 |
Description: Anti-PGII recombinant antibody |
Anti-PGII antibody |
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10-2809 | Fitzgerald | 1 mg | EUR 502.8 |
Description: Anti-PGII recombinant antibody |
Anti-CHW antibody |
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10-2810 | Fitzgerald | 1 mg | EUR 198 |
Description: Anti-CHW monoclonal antibody |
Anti-Giardia antibody |
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10-2811 | Fitzgerald | 1 mg | EUR 198 |
Description: Anti-Giardia monoclonal antibody |
Anti-Giardia antibody |
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10-2812 | Fitzgerald | 1 mg | EUR 198 |
Description: Anti-Giardia monoclonal antibody |
Anti-TAP1 Antibody |
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A01123 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal TAP1 Antibody. Validated in IP, IHC, WB and tested in Human, Rat. |
Anti-CHD4 Antibody |
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A01125 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal CHD4 Antibody. Validated in IF, IHC and tested in Human, Mouse. |
Anti-NRF1 Antibody |
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A01129-2 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-MEF2C Antibody |
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A01131-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-SCN4A Antibody |
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A01138 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal SCN4A Antibody. Validated in WB and tested in Human. |
Anti-MTR Antibody |
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A01140-2 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-IL12B Antibody |
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A01152-2 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-IL12B Antibody |
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A01152-3 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-IL12B Antibody |
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A01152-4 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-IL11 Antibody |
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A01160-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-Bak Antibody |
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A01163 | BosterBio | 200ug | EUR 626.4 |
Description: Rabbit Polyclonal Bak Antibody. Validated in IP, IF, IHC, WB and tested in Human. |
Anti-PGF Antibody |
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A01164-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-PGF Antibody |
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A01164-2 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-CD201 Antibody |
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A01166 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for CD201 Antibody (PROCR) detection.tested for IHC, WB in Human, Mouse, Rat. |
Anti-SERCA2 Antibody |
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A01176 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for SERCA2 Antibody (ATP2A2) detection.tested for IHC, WB in Human, Mouse, Rat. |
Anti-XPA Antibody |
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A01182 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal XPA Antibody. Validated in IHC, WB and tested in Human. |
Anti-XPA Antibody |
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A01182-1 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for XPA Antibody (XPA) detection.tested for WB in Human, Mouse. |
Anti-XPA Antibody |
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A01182-2 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-BNP Antibody |
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A01186-1 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal BNP Antibody. Validated in IF, IHC, WB and tested in Mouse, Rat. |
Anti-CD1c Antibody |
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A01188-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-CDC7 Antibody |
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A01190 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal CDC7 Antibody. Validated in WB and tested in Human. |
Anti-VCAM1 Antibody |
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A01199 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-VCAM1 Antibody |
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A01199-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-VCAM1 Antibody |
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A01199-2 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-MAP2 Antibody |
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A01201 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-MAP2 Antibody |
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A01201-1 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal MAP2 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat. |
Anti-RING1B Antibody |
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A01209-1 | BosterBio | 100ug | EUR 546 |
Description: Goat Polyclonal RING1B Antibody. Validated in IF, WB and tested in Human, Mouse, Rat. |
Anti-MUC2 Antibody |
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A01212 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-C1INH Antibody |
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A01221 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for C1INH Antibody (SERPING1) detection.tested for WB in Human. |
Anti-WWOX Antibody |
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A01223-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-RNF168 Antibody |
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A01224-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-HDAC5 Antibody |
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A01230-5 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-Dysferlin Antibody |
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A01234-1 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Dysferlin Antibody. Validated in WB and tested in Human, Mouse. |
Anti-LPP Antibody |
|||
A01240-2 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-p57 Antibody |
|||
A01244 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for p57 Antibody (CDKN1C) detection.tested for IHC, WB in Human, Mouse, Rat. |
Anti-MDC1 Antibody |
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A01252-2 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-FHL Antibody |
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A01258-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-FHL Antibody |
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A01258-2 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-ACBP Antibody |
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A01267-1 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for ACBP Antibody (DBI) detection. Tested with WB in Human, Mouse, Rat. |
Anti-PDK1 Antibody |
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A01268 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for PDK1 Antibody (PDK1) detection. Tested with WB in Human, Mouse, Rat. |
Anti-CD81 Antibody |
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A01281-1 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for CD81 Antibody (CD81) detection. Tested with WB in Human, Mouse, Rat. |
Anti-CHI3L1 Antibody |
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A01283-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-HLF Antibody |
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A01284-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-BRD7 Antibody |
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A01289-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-Endostatin Antibody |
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A01302 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Endostatin Antibody. Validated in IHC and tested in Human. |
Anti-COL18A1 Antibody |
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A01302-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-SATB1 Antibody |
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A01312-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-TCF7 Antibody |
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A01315-2 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-WEE1 Antibody |
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A01319-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-CD141 Antibody |
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A01325 | BosterBio | 100 ug | EUR 476.4 |
Description: Rabbit Polyclonal CD141 Antibody. Validated in WB and tested in Human. |
Anti-NDRG1 Antibody |
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A01327-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-FOXP2 Antibody |
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A01329-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-CTRO Antibody |
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A01331 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal CTRO Antibody. Validated in IHC and tested in Human. |
Anti-GOLPH3 Antibody |
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A01333-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-Aladin Antibody |
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A01336 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for Aladin Antibody (AAAS) detection. Tested with WB in Human, Rat. |
Anti-DBF4 Antibody |
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A01348-1 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal DBF4 Antibody. Validated in WB and tested in Human, Mouse. |
Anti-EIF4G1 Antibody |
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A01351-2 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-DDT Antibody |
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A01354 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-Cdc6 Antibody |
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A01355 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for Cdc6 Antibody (CDC6) detection.tested for WB in Human, Mouse. |
Anti-TRIM5 Antibody |
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A01359-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-STAT2 Antibody |
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A01360 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-CDC45L Antibody |
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A01367-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-VAPB Antibody |
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A01372 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-IGFBP2 Antibody |
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A01373-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-IGFBP2 Antibody |
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A01373-2 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-SphK2 Antibody |
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A01382 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for SphK2 Antibody (SPHK2) detection.tested for WB in Human, Mouse, Rat. |
Anti-SPHK2 Antibody |
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A01382-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-ITK Antibody |
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A01385-2 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-NAT1 Antibody |
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A01386-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-Tff1 Antibody |
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A01391-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-ALDH1A1 Antibody |
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A01392 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-TIP60 Antibody |
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A01393 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for TIP60 Antibody (KAT5) detection. Tested with WB in Human, Mouse, Rat. |
Anti-LIF Antibody |
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A01400 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-RALBP1 Antibody |
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A01403-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-Cav3.2 Antibody |
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A01406 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for Cav3.2 Antibody (CACNA1H) detection. Tested with IHC in Human, Rat, Mouse. |
Anti-CARD9 Antibody |
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A01410-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-PRMT1 Antibody |
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A01417-1 | BosterBio | 100ug | EUR 476.4 |
Description: Rabbit Polyclonal PRMT1 Antibody. Validated in ELISA, IHC, WB and tested in Human. |
Anti-PRMT1 Antibody |
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A01417-3 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-Lyn Antibody |
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A01424-2 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-IL6R Antibody |
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A01425-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-PSD Antibody |
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A01427 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-DDB2 Antibody |
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A01430-2 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-FCGR2A Antibody |
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A01450-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-FES Antibody |
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A01453 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-TAK1 Antibody |
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A01458 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Rabbit Polyclonal TAK1 Antibody. Validated in IHC and tested in Human, Mouse, Rat. |
Anti-GALT Antibody |
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A01460-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-BTG2 Antibody |
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A01461-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-GSTA1 Antibody |
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A01462 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for GSTA1 Antibody (GSTA1) detection.tested for IHC in Human. |
Anti-ACVL1 Antibody |
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A01468-1 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal ACVL1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
Anti-BNIP3 Antibody |
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A01469 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-TRPC3 Antibody |
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A01472 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-ABCG8 Antibody |
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A01482-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-ABCG8 Antibody |
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A01482-2 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-FEN1 Antibody |
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A01484 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal FEN1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat. |
Anti-FEN1 Antibody |
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A01484-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-ARHGEF5 Antibody |
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A01487-1 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal ARHGEF5 Antibody. Validated in WB and tested in Human, Mouse. |
Anti-Cdk8 Antibody |
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A01493 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for Cdk8 Antibody (CDK8) detection.tested for WB in Human, Mouse. |
Anti-Cdk8 Antibody |
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A01493-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-CRB1 Antibody |
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A01499 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-CRB1 Antibody |
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A01499-1 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-PARN Antibody |
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A01501-2 | BosterBio | 100ug/vial | EUR 400.8 |
Anti-HMBS Antibody |
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A01506-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-EPHB2 Antibody |
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A01507 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal EPHB2 Antibody. Validated in WB and tested in Human, Mouse, Rat. |
Anti-CD200 Antibody |
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A01512 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal Antibody for CD200 Antibody (CD200) detection.tested for IHC, WB in Human. |
Gemultiplexte en high-throughput labelvrije detectie van RNA/Spike Protein /IgG/IgM-biomarkers van SARS-CoV-2-infectie met behulp van nanoplasmonische biosensoren
Om de COVID-19-uitbraak, die wordt veroorzaakt door het ernstige acute respiratoire syndroom coronavirus 2 (SARS-CoV-2), aan te pakken, is er een onvervulde behoefte aan zeer nauwkeurige diagnostische checks in alle stadia van infectie met snelle resultaten en hoge specificiteit. Hier presenteren we een labelvrije, op nanoplasmonische biosensor gebaseerde, multiplex screeningtest voor COVID-19 die 10 verschillende biomarkers kwantitatief kan detecteren (6 virale nucleïnezuurgenen, 2 spike-eiwitsubeenheden en 2 antilichamen) met een detectielimiet in de aM-reeks, allemaal binnen één biosensorplatform.
Onze nieuw ontwikkelde nanoplasmonische biosensoren vertonen een hoge specificiteit, wat van het grootste belang is om valse reacties te voorkomen. Als proof of idea laten we zien dat onze detectiebenadering het potentieel heeft om zowel IgG- als IgM- antilichamen rechtstreeks uit COVID-19-positieve patiëntplasmamonsters te kwantificeren in een enkele instrumentrun, wat de hoge doorvoercapaciteit van onze detectiebenadering aantoont. Het belangrijkste is dat onze take a look at ontvangende operationele kenmerken biedt, gebieden onder de curve van respectievelijk 0,997 en 0,999 voor IgG en IgM. De berekende p- waarde bepaald met de Mann-Whitney niet-parametrische take a look at is <0,0001 voor beide antilichamen wanneer de take a look at van COVID-19-positieve patiënten ( n = 80) wordt vergeleken met die van gezonde personen ( n = 72).
Bovendien geeft de screeningstest een berekende sensitiviteit (true constructive fee) van 100% (80/80), een specificiteit (true negatieve fee) >96% (77/80) , een positief voorspellende waarde van 98% bij een prevalentie van 5% en een negatief voorspellende waarde van 100% bij een prevalentie van 5%. Wij zijn van mening dat onze zeer gevoelige, multiplex, high-throughput testbenadering potentiële toepassingen heeft in de COVID-19-diagnostiek , met title bij het bepalen van virusprogressie en infectie-ernst voor clinici voor een geschikte behandeling, en ook een zeer effectieve diagnostische take a look at zal blijken te zijn wanneer toegepast op ziekten buiten de COVID-19-pandemie.
Nauwkeurigheid van alternatieve niet-polariseerbare krachtvelden voor de bepaling van eiwit- ligandbindingsaffiniteiten gedomineerd door kation-π-interacties
Het aanpassen van paarspecifieke Lennard-Jones-parameters by way of de niet-gebonden FIX (NBFIX)-functie van het CHARMM36-krachtveld is kosteneffectief gebleken voor het verbeteren van de beschrijving van kation-π-interacties in biologische objecten door middel van paarsgewijs additieve potentiële energiefuncties. Hier worden twee units nieuw geoptimaliseerde CHARMM36-krachtveldparameters, waaronder NBFIX-correcties, bedacht CHARMM36m-NBF en CHARMM36-WYF, en de originele krachtvelden, namelijk CHARMM36m en Amber ff14SB, gebruikt om de standaard bindingsvrije energieën van zeven eiwit- ligandcomplexen die kation-π-interacties bevatten.
Vergeleken met nauwkeurige experimentele metingen, geven onze resultaten aan dat de ongecorrigeerde, originele krachtvelden de bindingsvrije energieën vital onderschatten, met een gemiddelde fout van 5,Three kcal/mol, terwijl de gemiddelde fouten van CHARMM36m-NBF en CHARMM36-WYF 0,eight en 2,1 bedragen. respectievelijk kcal/mol. De huidige studie toont overtuigend aan dat het gebruik van gewijzigde parameters samen met NBFIX-correcties de nauwkeurigheid van de standaard bindingsvrije energie van eiwit-ligandcomplexen die worden gedomineerd door kation-π-interacties, met title met CHARMM36m-NBF, dramatisch verhoogt.
Gebruikmakend van door tijd opgeloste, door proteïne geïnduceerde fluorescentieverbetering om stabiele lokale conformaties te identificeren, één α-synucleïne-monomeer per keer
Het gebruik van spectroscopische linialen om meerdere conformaties van afzonderlijke biomoleculen en hun dynamiek te volgen, heeft een revolutie teweeggebracht in het begrip van structurele dynamiek en zijn bijdragen aan de biologie. Terwijl de op FRET gebaseerde liniaal rapporteert over afstanden tussen kleurstoffen in het bereik van 3-10 nm, rapporteren andere spectroscopische technieken, zoals eiwit-geïnduceerde fluorescentieverbetering (PIFE), over de nabijheid tussen een kleurstof en een eiwitoppervlak in de kortere 0 -Three nm bereik. Ongeacht de gekozen methode, het gebruik ervan bij het één voor één meten van vrij diffunderende biomoleculen haalt histogrammen op van de experimentele parameter die afzonderlijke centraal verdeelde subpopulaties van biomoleculen oplevert, waarbij elke subpopulatie vertegenwoordigtofwel een enkele conformatie die binnen milliseconden onveranderd bleef, of meerdere conformaties die veel sneller dan milliseconden in elkaar overgaan, en dus een uitgemiddelde subpopulatie.
In FRET met één molecuul, waar de gerapporteerde parameter in histogrammen de FRET-efficiëntie tussen kleurstoffen is, werd eerder gerapporteerd dat een intrinsiek ongeordend eiwit, zoals het α-synucleïne-monomeer in buffer, een enkele gemiddelde subpopulatie van meerdere conformaties die snel in elkaar overgaan. Hoewel deze eerdere bevindingen afhankelijk zijn van het bereik van 3-10 nm van de FRET-gebaseerde liniaal, hebben we geprobeerd dit eiwit op de proef te stellen met behulp van PIFE met één molecuul, waarbij we de fluorescentielevensduur van plaatsspecifieke sCy3-gelabelde α-Synuclein volgen. eiwitten één voor één. Interessant is dat met behulp van deze spectroscopische naderingssensor met een kortere afstand, sCy3-gelabeld -Synucleïne verschillende subpopulaties met een duidelijk verschillende gemiddelde levensduur vertoont die in 10-100 ms in elkaar overgaan.Deze resultaten laten zien dat, hoewel α-synucleïne wereldwijd ontregeld kan zijn, het toch stabiele lokale structuren bereikt. Samenvattend, in dit werk benadrukken we het voordeel van het gebruik van verschillende spectroscopische nabijheidssensoren die lokale of globale structurele veranderingen één biomolecuul tegelijk volgen.