SPC1 regelt de signaal-peptidase bemiddelde verwerken van membraan  proteïnen

Signaalpeptidase (SPase) splitst de signaalsequenties (SS’s ) van secretoire voorlopers. Het bevat een evolutionair geconserveerde membraaneiwitsubeenheid, Spcl, die niet nodig is voor de katalytische activiteit van SPase, en de rol ervan blijft onbekend. In de huidige studie hebben we de functie van gist Spc1 onderzocht. Eerst hebben we een in vivo SPase-splitsingstest opgezet met behulp van secretoire eiwit-CPY- varianten met SS’s gemodificeerd in de n- en h-regio’s.

Bij het vergelijken van de SS-splitsingsefficiëntie van deze varianten in cellen met of zonder Spcl, ontdekten we dat signaalverankerde sequenties vatbaarder werden voor splitsing door SPase zonder Spcl. Verder was SPase-gemedieerde verwerking van modelmembraaneiwitten verbeterd in de afwezigheid van maar verminderd bij overexpressie van Spcl. Spcl werd geco-immunoprecipiteerd met eiwitten die niet-gesplitste signaalverankerde of transmembraan (TM) segmenten droegen.

Samengevat suggereren deze resultaten dat Spc1 TM-segmenten beschermt tegen SPase-actie, waardoor de substraatselectie voor SPase wordt verscherpt en fungeert als een negatieve regulator voor de SPase-gemedieerde verwerking van membraaneiwitten .

Interleukine-10 speelt een belangrijke rol in pasgeboren ratten met Hypoxic-ischemie geassocieerd met B-cel lymfoom 2 en endoplasmatisch reticulum  Protein  29

 

Interleukine 10 (IL-10) is een synthetische remmer van humane cytokines met immunomodulerende en ontstekingsremmende effecten. Deze studie was bedoeld om de expressievariatie van IL-10 op meerdere plaatsen, waaronder cortex, hippocampus en longweefsels van neonatale hypoxisch-ischemische (HI) ratten te onderzoeken en de cruciale rol van IL-10 bij het verlichten van HI-hersenschade te onderzoeken. In deze studie werden neonatale Sprague-Dawley-ratten onderworpen aan de ligatie van de rechter gemeenschappelijke halsslagader, gevolgd door 2 uur hypoxie.

De expressie van IL-10 in de cortex, hippocampus en longweefsels werd gemeten met immunohistochemie, realtime kwantitatieve polymerasekettingreactie (RT-qPCR) en western blot (WB). Dubbele kleuring met immunofluorescentie werd uitgevoerd om de lokalisatie van IL-10 in neuronen en astrocyten te observeren . Bovendien werden niet-targeting en concentrating on IL-10 siRNA-lentivirusvectoren geïnjecteerd in respectievelijk de ratten van de negatieve controle (NC) en IL-10-groep, en de mRNA-niveaus van B-cellymfoom 2 (Bcl-2) en endoplasmatische reticulum eiwit 29 (ERp29) werden gedetecteerd door RT-qPCR na IL-10 stilte.

De resultaten toonden aan dat de IL-10-expressie aanzienlijk was verhoogd nadat HI en IL-10 waren gecolokaliseerd met neuronen en astrocyten die ernstig waren beschadigd door HI-belediging. Bovendien waren Bcl-2 en ERp29 opmerkelijk verlaagd na IL-10-mRNA-interferentie in vergelijking met de NC-groep. Onze bevindingen onthulden dat IL-10 zijn anti-apoptotische en neuroprotectieve effecten uitoefende door de expressie van Bcl-2 en ERp29 te reguleren, wat aangeeft dat IL-10 een veelbelovend molecuuldoelwit kan zijn voor HIE-behandeling.

GCFC2 siRNA
20-abx917674	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

GCFC2 siRNA
20-abx917675	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
Shipped within 5-10 working days.

GCFC2 Blocking Peptide
20-abx063333	Abbexa	EUR 272.00 EUR 411.00	1 mg 5 mg
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Human GCFC2 shRNA Plasmid
20-abx954755	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
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Mouse GCFC2 shRNA Plasmid
20-abx983608	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
Shipped within 15-20 working days.

pCMV-SPORT6-GCFC2-G181A
PVT12797	Lifescience Market	2 ug	EUR 391

GCFC2 Recombinant Protein (Human)
RP060264	ABM	100 ug	Ask for price

GCFC2 Recombinant Protein (Rat)
RP202352	ABM	100 ug	Ask for price

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 280

Polyclonal Goat anti-GST p-form
GST-ANTI-3	Detroit R&D	50 uL	EUR 280

Gcfc2 ORF Vector (Rat) (pORF)
ORF067452	ABM	1.0 ug DNA	EUR 506

GCFC2 ORF Vector (Human) (pORF)
ORF020089	ABM	1.0 ug DNA	Ask for price

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody
20-abx008656	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul
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Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody
abx330966-100ul	Abbexa	100 ul	EUR 425
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Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody
20-abx323787	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug
Shipped within 5-10 working days.

GCFC2 Protein Vector (Rat) (pPB-C-His)
PV269806	ABM	500 ng	EUR 1166

GCFC2 Protein Vector (Rat) (pPB-N-His)
PV269807	ABM	500 ng	EUR 1166

GCFC2 Protein Vector (Rat) (pPM-C-HA)
PV269808	ABM	500 ng	EUR 1166

GCFC2 Protein Vector (Rat) (pPM-C-His)
PV269809	ABM	500 ng	EUR 1166

GCFC2 Protein Vector (Human) (pPB-C-His)
PV080353	ABM	500 ng	Ask for price

GCFC2 Protein Vector (Human) (pPB-N-His)
PV080354	ABM	500 ng	Ask for price

GCFC2 Protein Vector (Human) (pPM-C-HA)
PV080355	ABM	500 ng	Ask for price

GCFC2 Protein Vector (Human) (pPM-C-His)
PV080356	ABM	500 ng	Ask for price

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV654355	ABM	1.0 ug DNA	EUR 1355

GCFC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV654359	ABM	1.0 ug DNA	EUR 1355

GCFC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV654360	ABM	1.0 ug DNA	EUR 1355

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
LV762095	ABM	1.0 ug DNA	EUR 920

GCFC2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV762099	ABM	1.0 ug DNA	EUR 920

GCFC2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV762100	ABM	1.0 ug DNA	EUR 920

Human Chromosome 2 Open Reading Frame 3 (GCFC2) ELISA Kit
abx386199-96tests	Abbexa	96 tests	EUR 911
Shipped within 5-12 working days.

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV654356	ABM	1.0 ug DNA	EUR 1355

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV654357	ABM	1.0 ug DNA	EUR 1413

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV654358	ABM	1.0 ug DNA	EUR 1413

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV762096	ABM	1.0 ug DNA	EUR 920

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV762097	ABM	1.0 ug DNA	EUR 978

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV762098	ABM	1.0 ug DNA	EUR 978

Anti-Anti-SEPT6 antibody antibody
STJ11100949	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody
STJ111369	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT11 Antibody antibody
STJ111530	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT4 Antibody antibody
STJ112276	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-SEPT2 Antibody antibody
STJ25475	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT5 Antibody antibody
STJ25477	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody
STJ25479	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT2 Antibody antibody
STJ28365	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT7 Antibody antibody
STJ28963	St John's Laboratory	100 µl	EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-MARCH9 Antibody antibody
STJ112609	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT11 Antibody antibody
STJ113941	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT11 Antibody antibody
STJ114081	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT5 Antibody antibody
STJ114819	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-MARCH8 Antibody antibody
STJ114828	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT7 Antibody antibody
STJ116214	St John's Laboratory	100 µl	EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody
STJ117206	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody
STJ117759	St John's Laboratory	100 µl	EUR 277
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody
STJ118549	St John's Laboratory	100 µl	EUR 277

Anti-Anti-MARCH6 Antibody antibody
STJ118550	St John's Laboratory	100 µl	EUR 277

Anti-Anti-MARCH7 Antibody antibody
STJ118752	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT3 Antibody antibody
STJ118990	St John's Laboratory	100 µl	EUR 277

Anti-Anti-SEPT1 antibody antibody
STJ119580	St John's Laboratory	100 µl	EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-DDB1 Antibody
A00333	BosterBio	100uL	EUR 455
Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5647.8
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Int Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 552.76
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Int Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 746.8
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Int Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3060.6
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Int Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
4-AEA465Hu	Cloud-Clone	EUR 5698.00 EUR 3011.00 EUR 747.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Known also as Anti-Sperm Antibody elisa. Alternative names of the recognized antigen: Anti-Spermatozoa Antibodies Sperm Antibodies Antisperm Antibodies
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)
ELK8071	ELK Biotech	1 plate of 96 wells	EUR 432
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate soluti Show more
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Anti-CYFIP2 Antibody
A06562	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for CYFIP2 Antibody (CYFIP2) detection.tested for IHC, WB in Human, Mouse, Rat.

Anti-GPS2 Antibody
A06569	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for GPS2 Antibody (GPS2) detection. Tested with WB in Human, Mouse, Rat.

Anti-PRKX Antibody
A06585-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal PRKX Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-PPA2 Antibody
A06587	BosterBio	100ug/vial	EUR 294

Anti-GluR8 Antibody
A06589	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal GluR8 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-EDG7 Antibody
A06597-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal EDG7 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-KIR2.3 Antibody
A06605-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for KIR2.3 Antibody (KCNJ4) detection. Tested with WB in Human, Mouse, Rat.

Anti-GALK1 Antibody
A06627	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal GALK1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-PRAME Antibody
A06628-2	BosterBio	100ug/vial	EUR 294

Anti-GP2 Antibody
A06630-1	BosterBio	100ug/vial	EUR 334

Anti-USP21 Antibody
A06639	BosterBio	100ug/200ul	EUR 397
Description: Goat Polyclonal USP21 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-TLK2 Antibody
A06645	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for TLK2 Antibody (TLK2) detection. Tested with WB in Human, Mouse.

Anti-PPP1R3C Antibody
A06658	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal PPP1R3C Antibody. Validated in WB and tested in Human.

Anti-LRRK1 Antibody
A06670	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal LRRK1 Antibody. Validated in IF, IHC and tested in Human.

Anti-MNDA Antibody
A06675	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal MNDA Antibody. Validated in WB and tested in Human.

Anti-KAL1 Antibody
A06684	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal KAL1 Antibody. Validated in WB and tested in Human.

Anti-COL14A1 Antibody
A06685	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for COL14A1 Antibody (COL14A1) detection.tested for IHC in Human, Mouse, Rat.

Anti-CYP4B1 Antibody
A06690	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for CYP4B1 Antibody (CYP4B1) detection.tested for WB in Human.

Anti-RFX2 Antibody
A06709	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal RFX2 Antibody. Validated in IHC and tested in Human.

Anti-GPR92 Antibody
A06721	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for GPR92 Antibody (LPAR5) detection. Tested for WB, IHC, IF in Human.

Anti-MBTPS1 Antibody
A06735	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal MBTPS1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-PLA2G1B Antibody
A06738	BosterBio	100ug/200ul	EUR 397
Description: Goat Polyclonal PLA2G1B Antibody. Validated in WB and tested in Human.

Anti-GNB5 Antibody
A06754	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal GNB5 Antibody. Validated in IHC, WB and tested in Human.

Anti-BAIAP2L1 Antibody
A06770	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal BAIAP2L1 Antibody. Validated in WB and tested in Human.

Anti-IRTKS Antibody
A06770-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for IRTKS Antibody (BAIAP2L1) detection.tested for WB in Human, Mouse, Rat.

Anti-MLF1 Antibody
A06772	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal MLF1 Antibody. Validated in WB and tested in Human, Mouse.

Anti-NDR2 Antibody
A06774	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for NDR2 Antibody (STK38L) detection.tested for WB in Human, Mouse, Rat.

Anti-LARG Antibody
A06802-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for LARG Antibody (ARHGEF12) detection. Tested with WB in Human, Mouse, Rat.

Anti-GAS1 Antibody
A06815-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal GAS1 Antibody. Validated in WB and tested in Human, Mouse.

Anti-APLF Antibody
A06828-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for APLF Antibody (APLF) detection.tested for WB in Human, Mouse.

Anti-PKCB1 Antibody
A06830	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal PKCB1 Antibody. Validated in WB and tested in Human.

Anti-DnaJB4 Antibody
A06835-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for DnaJB4 Antibody (DNAJB4) detection.tested for WB in Human, Mouse, Rat.

Anti-ArfGAP3 Antibody
A06839	BosterBio	100uL	EUR 455
Description: Rabbit Polyclonal ArfGAP3 Antibody. Validated in IP, IF, WB and tested in Human.

Anti-SCN2B Antibody
A06842	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal SCN2B Antibody. Validated in WB and tested in Human.

Anti-CD316 Antibody
A06844	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for CD316 Antibody (IGSF8) detection. Tested with WB in Human, Mouse.

Anti-AMOTL2 Antibody
A06852	BosterBio	100ug/vial	EUR 294

Anti-POM121 Antibody
A06856	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal POM121 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-TBX22 Antibody
A06861	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal TBX22 Antibody. Validated in WB and tested in Human.

Anti-QORX Antibody
A06870	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal QORX Antibody. Validated in WB and tested in Human.

Anti-ADAM28 Antibody
A06873-1	BosterBio	100ug/vial	EUR 294

Anti-Adam28 Antibody
A06873-2	BosterBio	100ug/vial	EUR 334

Anti-KSR2 Antibody
A06876	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal KSR2 Antibody. Validated in IF, WB and tested in Human, Mouse.

Anti-Recoverin Antibody
A06882	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for Recoverin Antibody (RCVRN) detection.tested for WB in Human, Mouse.

Anti-CRP1 Antibody
A06894	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal CRP1 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-CD297 Antibody
A06913	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for CD297 Antibody (ART4) detection.tested for IHC in Human.

Anti-TAF12 Antibody
A06944-1	BosterBio	100ug/vial	EUR 334

Anti-AKR7A2 Antibody
A06949-1	BosterBio	100ug/vial	EUR 334

Anti-IPMK Antibody
A06955	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal IPMK Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-ARHGEF10 Antibody
A06958	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for ARHGEF10 Antibody (ARHGEF10) detection.tested for WB in Human, Monkey.

Anti-BMP10 Antibody
A06968	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal BMP10 Antibody. Validated in WB and tested in Human.

Anti-SGK269 Antibody
A06989	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal SGK269 Antibody. Validated in WB and tested in Human, Mouse.

Anti-MAST205 Antibody
A07003	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for MAST205 Antibody (MAST2) detection.tested for WB in Human, Mouse.

Anti-TFF2 Antibody
A07013-1	BosterBio	100ug/vial	EUR 334

Anti-TFF2 Antibody
A07013-2	BosterBio	100ug/vial	EUR 334

Anti-FOXK1 Antibody
A07015	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal FOXK1 Antibody. Validated in WB and tested in Human, Mouse.

Anti-CD158z Antibody
A07017	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for CD158z Antibody (KIR3DL3) detection.tested for IHC, WB in Human.

Anti-TSEN54 Antibody
A07022	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal TSEN54 Antibody. Validated in IHC, WB and tested in Human, Mouse.

Anti-SAR1B Antibody
A07030	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal SAR1B Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-Peropsin Antibody
A07038	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for Peropsin Antibody (RRH) detection. Tested with WB in Human.

Anti-RALY Antibody
A07043	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal RALY Antibody. Validated in WB and tested in Human.

Anti-Raly Antibody
A07043-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for Raly Antibody (RALY) detection. Tested with WB in Human, Mouse, Rat.

Anti-Osteoglycin Antibody
A07061	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for Osteoglycin Antibody (OGN) detection. Tested with WB in Human, Mouse.

Anti-APC10 Antibody
A07065-1	BosterBio	100ug	EUR 455
Description: Rabbit Polyclonal APC10 Antibody. Validated in IP, WB and tested in Human.

Anti-RRBP1 Antibody
A07074-1	BosterBio	100ug/vial	EUR 334

Anti-DGKH Antibody
A07077-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal DGKH Antibody. Validated in IF, IHC and tested in Human, Mouse.

Anti-NUP93 Antibody
A07090	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal NUP93 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-RBM20 Antibody
A07094	BosterBio	100ug/200ul	EUR 397
Description: Goat Polyclonal RBM20 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-RhoH Antibody
A07101	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal RhoH Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-ZNF265 Antibody
A07103	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal ZNF265 Antibody. Validated in IF, WB and tested in Human.

Anti-ZIS Antibody
A07103-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for ZIS Antibody (ZRANB2) detection.tested for WB in Human, Mouse, Rat.

Anti-BCAS3 Antibody
A07120	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for BCAS3 Antibody (BCAS3) detection. Tested with WB in Human, Mouse.

Anti-TAF3 Antibody
A07129	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal TAF3 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-TIMP4 Antibody
A07131	BosterBio	100ug/vial	EUR 334

Anti-NIPA Antibody
A07135	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for NIPA Antibody (ZC3HC1) detection.tested for WB in Human, Mouse, Rat, Monkey.

Anti-ATP1AL1 Antibody
A07149	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for ATP1AL1 Antibody (ATP12A) detection. Tested with WB in Human, Rat.

Anti-HoxB5 Antibody
A07156	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for HoxB5 Antibody (HOXB5) detection. Tested with WB in Human, Mouse.

Anti-ARRDC3 Antibody
A07177	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal ARRDC3 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-RGS5 Antibody
A07178	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal RGS5 Antibody. Validated in IHC, WB and tested in Human, Mouse.

Anti-MLF1 Antibody
A07180	BosterBio	100uL	EUR 443
Description: Rabbit Polyclonal MLF1 Antibody. Validated in IHC, WB and tested in Human.

Anti-G2A Antibody
A07182	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for G2A Antibody (GPR132) detection.tested for WB in Human, Mouse, Monkey.

Anti-NICE4 Antibody
A07183	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for NICE4 Antibody (UBAP2L) detection. Tested with WB in Human, Mouse.

Anti-USP36 Antibody
A07184	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal USP36 Antibody. Validated in IHC and tested in Human.

Anti-GPR1 Antibody
A07199	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal GPR1 Antibody. Validated in IF and tested in Human, Mouse, Rat.

Anti-Tis7 Antibody
A07201	BosterBio	100 ug	EUR 397
Description: Rabbit Polyclonal Tis7 Antibody. Validated in WB and tested in Human.

Anti-APEX2 Antibody
A07203	BosterBio	100ug/vial	EUR 294

Anti-TRIM59 Antibody
A07207	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal TRIM59 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse.

Anti-GCN5 Antibody
A07210	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for GCN5 Antibody (KAT2A) detection.tested for WB in Human, Mouse.

Anti-CNT2 Antibody
A07211-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for CNT2 Antibody (SLC28A2) detection.tested for WB in Human, Mouse, Rat.

Anti-D54 Antibody
A07219-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for D54 Antibody (TPD52L2) detection. Tested with WB in Human, Mouse, Rat.

Anti-ST7 Antibody
A07235	BosterBio	100ug/vial	EUR 294

Anti-RAB3GAP2 Antibody
A07244	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal RAB3GAP2 Antibody. Validated in IHC, WB and tested in Human, Mouse.

Anti-RAB3GAP2 Antibody
A07244-3	BosterBio	100ug/vial	EUR 334

Anti-LYPLA1 Antibody
A07249	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal LYPLA1 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-ASPP1 Antibody
A07257	BosterBio	100ug	EUR 432
Description: Rabbit Polyclonal ASPP1 Antibody. Validated in WB and tested in Human.

Anti-IRX3 Antibody
A07258	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal IRX3 Antibody. Validated in IHC and tested in Human, Mouse.

Anti-SNAPC5 Antibody
A07260	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal SNAPC5 Antibody. Validated in IHC and tested in Human, Mouse.

Anti-ERp72 Antibody
A07267	BosterBio	200ug/vial	EUR 455
Description: Rabbit Polyclonal ERp72 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-PBK1 Antibody
A07282	BosterBio	100ug	EUR 455
Description: Rabbit Polyclonal PBK1 Antibody. Validated in IF, WB and tested in Human.

Anti-THEM2 Antibody
A07288	BosterBio	100 ug	EUR 397
Description: Rabbit Polyclonal THEM2 Antibody. Validated in WB and tested in Human.

Anti-A1BG Antibody
A07289	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for A1BG Antibody (A1BG) detection.tested for IHC, WB in Human.

Anti-SFRS4 Antibody
A07291	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal SFRS4 Antibody. Validated in WB and tested in Human, Mouse.

Anti-CDCA2 Antibody
A07293	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal CDCA2 Antibody. Validated in WB and tested in Human.

Anti-AASS Antibody
A07302	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for AASS Antibody (AASS) detection. Tested with WB in Human.

Anti-PSG1 Antibody
A07308	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for PSG1 Antibody (PSG1) detection.tested for IHC, WB in Human.

Anti-RGS12 Antibody
A07317	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal RGS12 Antibody. Validated in WB and tested in Human, Mouse.

Anti-RGS12 Antibody
A07317-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for RGS12 Antibody (RGS12) detection. Tested with WB in Human, Mouse, Rat.

Anti-SSX Antibody
A07363	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for SSX Antibody (SSX1) detection.tested for IHC, WB in Human.

Anti-MUC20 Antibody
A07372	BosterBio	100ug/vial	EUR 334

Anti-SLC10A7 Antibody
A07374-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal SLC10A7 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-STK33 Antibody
A07375	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for STK33 Antibody (STK33) detection.tested for WB in Human.

Anti-GPR119 Antibody
A07391	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for GPR119 Antibody (GPR119) detection.tested for WB in Human.

Anti-NAT8L Antibody
A07393-2	BosterBio	100ug/vial	EUR 294

Anti-Bag5 Antibody
A07402	BosterBio	100ug/vial	EUR 294

Anti-CIDEB Antibody
A07404	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal CIDEB Antibody. Validated in IF, IHC, WB and tested in Human, Mouse.

Anti-IFIT5 Antibody
A07415	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal IFIT5 Antibody. Validated in WB and tested in Human.

Anti-NDUFA9 Antibody
A07420	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal NDUFA9 Antibody. Validated in WB and tested in Human.

Anti-Dematin Antibody
A07422	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for Dematin Antibody (DMTN) detection.tested for WB in Human, Mouse.

Anti-ZP4 Antibody
A07440	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal ZP4 Antibody. Validated in WB and tested in Human.

Anti-JTB Antibody
A07448	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for JTB Antibody (JTB) detection. Tested with WB in Human.

Anti-MRCKB Antibody
A07454	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal MRCKB Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-PACRG Antibody
A07487	BosterBio	100ug	EUR 432
Description: Rabbit Polyclonal PACRG Antibody. Validated in WB and tested in Human.

Anti-TUSC2 Antibody
A07508	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for TUSC2 Antibody (TUSC2) detection.tested for WB in Human, Mouse.

Anti-FOXI1 Antibody
A07512-1	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal FOXI1 Antibody. Validated in IHC and tested in Human, Mouse.

Anti-FOXI1 Antibody
A07512-3	BosterBio	100ug/vial	EUR 334

Gemultiplexte en high-throughput labelvrije detectie van RNA/Spike  Protein /IgG/IgM-biomarkers van SARS-CoV-2-infectie met behulp van nanoplasmonische biosensoren

Om de COVID-19-uitbraak, die wordt veroorzaakt door het ernstige acute respiratoire syndroom coronavirus 2 (SARS-CoV-2), aan te pakken, is er een onvervulde behoefte aan zeer nauwkeurige diagnostische checks in alle stadia van infectie met snelle resultaten en hoge specificiteit. Hier presenteren we een labelvrije, op nanoplasmonische biosensor gebaseerde, multiplex screeningtest voor COVID-19 die 10 verschillende biomarkers kwantitatief kan detecteren (6 virale nucleïnezuurgenen, 2 spike-eiwitsubeenheden en 2 antilichamen) met een detectielimiet in de aM-reeks, allemaal binnen één biosensorplatform.

Onze nieuw ontwikkelde nanoplasmonische biosensoren vertonen een hoge specificiteit, wat van het grootste belang is om valse reacties te voorkomen. Als proof of idea laten we zien dat onze detectiebenadering het potentieel heeft om zowel IgG- als IgM- antilichamen rechtstreeks uit COVID-19-positieve patiëntplasmamonsters te kwantificeren in een enkele instrumentrun, wat de hoge doorvoercapaciteit van onze detectiebenadering aantoont. Het belangrijkste is dat onze take a look at ontvangende operationele kenmerken biedt, gebieden onder de curve van respectievelijk 0,997 en 0,999 voor IgG en IgM. De berekende  p- waarde bepaald met de Mann-Whitney niet-parametrische take a look at is <0,0001 voor beide antilichamen wanneer de take a look at van COVID-19-positieve patiënten ( n = 80) wordt vergeleken met die van gezonde personen ( n  = 72).

Bovendien geeft de screeningstest een berekende sensitiviteit (true constructive fee) van 100% (80/80), een specificiteit (true negatieve fee) >96% (77/80) , een positief voorspellende waarde van 98% bij een prevalentie van 5% en een negatief voorspellende waarde van 100% bij een prevalentie van 5%. Wij zijn van mening dat onze zeer gevoelige, multiplex, high-throughput testbenadering potentiële toepassingen heeft in de COVID-19-diagnostiek , met title bij het bepalen van virusprogressie en infectie-ernst voor clinici voor een geschikte behandeling, en ook een zeer effectieve diagnostische take a look at zal blijken te zijn wanneer toegepast op ziekten buiten de COVID-19-pandemie.

Nauwkeurigheid van alternatieve niet-polariseerbare krachtvelden voor de bepaling van  eiwit- ligandbindingsaffiniteiten gedomineerd door kation-π-interacties

Het aanpassen van paarspecifieke Lennard-Jones-parameters by way of de niet-gebonden FIX (NBFIX)-functie van het CHARMM36-krachtveld is kosteneffectief gebleken voor het verbeteren van de beschrijving van kation-π-interacties in biologische objecten door middel van paarsgewijs additieve potentiële energiefuncties. Hier worden twee units nieuw geoptimaliseerde CHARMM36-krachtveldparameters, waaronder NBFIX-correcties, bedacht CHARMM36m-NBF en CHARMM36-WYF, en de originele krachtvelden, namelijk CHARMM36m en Amber ff14SB, gebruikt om de standaard bindingsvrije energieën van zeven eiwit- ligandcomplexen die kation-π-interacties bevatten.

Vergeleken met nauwkeurige experimentele metingen, geven onze resultaten aan dat de ongecorrigeerde, originele krachtvelden de bindingsvrije energieën vital onderschatten, met een gemiddelde fout van 5,Three kcal/mol, terwijl de gemiddelde fouten van CHARMM36m-NBF en CHARMM36-WYF 0,eight en 2,1 bedragen. respectievelijk kcal/mol. De huidige studie toont overtuigend aan dat het gebruik van gewijzigde parameters samen met NBFIX-correcties de nauwkeurigheid van de standaard bindingsvrije energie van eiwit-ligandcomplexen die worden gedomineerd door kation-π-interacties, met title met CHARMM36m-NBF, dramatisch verhoogt.

Gebruikmakend van door tijd opgeloste, door  proteïne geïnduceerde fluorescentieverbetering om stabiele lokale conformaties te identificeren, één α-synucleïne-monomeer per keer

Het gebruik van spectroscopische linialen om meerdere conformaties van afzonderlijke biomoleculen en hun dynamiek te volgen, heeft een revolutie teweeggebracht in het begrip van structurele dynamiek en zijn bijdragen aan de biologie. Terwijl de op FRET gebaseerde liniaal rapporteert over afstanden tussen kleurstoffen in het bereik van 3-10 nm, rapporteren andere spectroscopische technieken, zoals eiwit-geïnduceerde fluorescentieverbetering (PIFE), over de nabijheid tussen een kleurstof en een eiwitoppervlak in de kortere 0 -Three nm bereik. Ongeacht de gekozen methode, het gebruik ervan bij het één voor één meten van vrij diffunderende biomoleculen haalt histogrammen op van de experimentele parameter die afzonderlijke centraal verdeelde subpopulaties van biomoleculen oplevert, waarbij elke subpopulatie vertegenwoordigtofwel een enkele conformatie die binnen milliseconden onveranderd bleef, of meerdere conformaties die veel sneller dan milliseconden in elkaar overgaan, en dus een uitgemiddelde subpopulatie.

In FRET met één molecuul, waar de gerapporteerde parameter in histogrammen de FRET-efficiëntie tussen kleurstoffen is, werd eerder gerapporteerd dat een intrinsiek ongeordend eiwit, zoals het α-synucleïne-monomeer in buffer, een enkele gemiddelde subpopulatie van meerdere conformaties die snel in elkaar overgaan. Hoewel deze eerdere bevindingen afhankelijk zijn van het bereik van 3-10 nm van de FRET-gebaseerde liniaal, hebben we geprobeerd dit eiwit op de proef te stellen met behulp van PIFE met één molecuul, waarbij we de fluorescentielevensduur van plaatsspecifieke sCy3-gelabelde α-Synuclein volgen. eiwitten één voor één. Interessant is dat met behulp van deze spectroscopische naderingssensor met een kortere afstand, sCy3-gelabeld -Synucleïne verschillende subpopulaties met een duidelijk verschillende gemiddelde levensduur vertoont die in 10-100 ms in elkaar overgaan.Deze resultaten laten zien dat, hoewel α-synucleïne wereldwijd ontregeld kan zijn, het toch stabiele lokale structuren bereikt. Samenvattend, in dit werk benadrukken we het voordeel van het gebruik van verschillende spectroscopische nabijheidssensoren die lokale of globale structurele veranderingen één biomolecuul tegelijk volgen.