SPC1 regelt de signaal-peptidase bemiddelde verwerken van membraan  proteïnen

Signaalpeptidase (SPase) splitst de signaalsequenties (SS’s ) van secretoire voorlopers. Het bevat een evolutionair geconserveerde membraaneiwitsubeenheid, Spcl, die niet nodig is voor de katalytische activiteit van SPase, en de rol ervan blijft onbekend. In de huidige studie hebben we de functie van gist Spc1 onderzocht. Eerst hebben we een in vivo SPase-splitsingstest opgezet met behulp van secretoire eiwit-CPY- varianten met SS’s gemodificeerd in de n- en h-regio’s.

Bij het vergelijken van de SS-splitsingsefficiëntie van deze varianten in cellen met of zonder Spcl, ontdekten we dat signaalverankerde sequenties vatbaarder werden voor splitsing door SPase zonder Spcl. Verder was SPase-gemedieerde verwerking van modelmembraaneiwitten verbeterd in de afwezigheid van maar verminderd bij overexpressie van Spcl. Spcl werd geco-immunoprecipiteerd met eiwitten die niet-gesplitste signaalverankerde of transmembraan (TM) segmenten droegen.

Samengevat suggereren deze resultaten dat Spc1 TM-segmenten beschermt tegen SPase-actie, waardoor de substraatselectie voor SPase wordt verscherpt en fungeert als een negatieve regulator voor de SPase-gemedieerde verwerking van membraaneiwitten .

Interleukine-10 speelt een belangrijke rol in pasgeboren ratten met Hypoxic-ischemie geassocieerd met B-cel lymfoom 2 en endoplasmatisch reticulum  Protein  29

 

Interleukine 10 (IL-10) is een synthetische remmer van humane cytokines met immunomodulerende en ontstekingsremmende effecten. Deze studie was bedoeld om de expressievariatie van IL-10 op meerdere plaatsen, waaronder cortex, hippocampus en longweefsels van neonatale hypoxisch-ischemische (HI) ratten te onderzoeken en de cruciale rol van IL-10 bij het verlichten van HI-hersenschade te onderzoeken. In deze studie werden neonatale Sprague-Dawley-ratten onderworpen aan de ligatie van de rechter gemeenschappelijke halsslagader, gevolgd door 2 uur hypoxie.

De expressie van IL-10 in de cortex, hippocampus en longweefsels werd gemeten met immunohistochemie, realtime kwantitatieve polymerasekettingreactie (RT-qPCR) en western blot (WB). Dubbele kleuring met immunofluorescentie werd uitgevoerd om de lokalisatie van IL-10 in neuronen en astrocyten te observeren . Bovendien werden niet-targeting en concentrating on IL-10 siRNA-lentivirusvectoren geïnjecteerd in respectievelijk de ratten van de negatieve controle (NC) en IL-10-groep, en de mRNA-niveaus van B-cellymfoom 2 (Bcl-2) en endoplasmatische reticulum eiwit 29 (ERp29) werden gedetecteerd door RT-qPCR na IL-10 stilte.

De resultaten toonden aan dat de IL-10-expressie aanzienlijk was verhoogd nadat HI en IL-10 waren gecolokaliseerd met neuronen en astrocyten die ernstig waren beschadigd door HI-belediging. Bovendien waren Bcl-2 en ERp29 opmerkelijk verlaagd na IL-10-mRNA-interferentie in vergelijking met de NC-groep. Onze bevindingen onthulden dat IL-10 zijn anti-apoptotische en neuroprotectieve effecten uitoefende door de expressie van Bcl-2 en ERp29 te reguleren, wat aangeeft dat IL-10 een veelbelovend molecuuldoelwit kan zijn voor HIE-behandeling.

GCFC2 Blocking Peptide
20-abx063333	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

Human GCFC2 shRNA Plasmid
20-abx954755	Abbexa	EUR 961.20 EUR 1345.20	150 µg 300 µg

Mouse GCFC2 shRNA Plasmid
20-abx983608	Abbexa	EUR 961.20 EUR 1345.20	150 µg 300 µg

GCFC2 Recombinant Protein (Rat)
RP202352	ABM	100 ug	Ask for price

GCFC2 Recombinant Protein (Human)
RP060264	ABM	100 ug	Ask for price

Gcfc2 ORF Vector (Rat) (pORF)
ORF067452	ABM	1.0 ug DNA	EUR 607.2

GCFC2 ORF Vector (Human) (pORF)
ORF020089	ABM	1.0 ug DNA	Ask for price

Human GCFC2 Protein Lysate 20ug
IHUGCFC2PLLY20UG	Innovative research	each	EUR 213
Description: Human GCFC2 Protein Lysate 20ug

GCFC2 Protein Vector (Rat) (pPM-C-HA)
PV269808	ABM	500 ng	EUR 1399.2

GCFC2 Protein Vector (Rat) (pPB-C-His)
PV269806	ABM	500 ng	EUR 1399.2

GCFC2 Protein Vector (Rat) (pPB-N-His)
PV269807	ABM	500 ng	EUR 1399.2

GCFC2 Protein Vector (Rat) (pPM-C-His)
PV269809	ABM	500 ng	EUR 1399.2

GCFC2 Protein Vector (Human) (pPM-C-HA)
PV080355	ABM	500 ng	Ask for price

GCFC2 Protein Vector (Human) (pPB-C-His)
PV080353	ABM	500 ng	Ask for price

GCFC2 Protein Vector (Human) (pPB-N-His)
PV080354	ABM	500 ng	Ask for price

GCFC2 Protein Vector (Human) (pPM-C-His)
PV080356	ABM	500 ng	Ask for price

GCFC1 Antibody
45987-100ul	SAB	100ul	EUR 302.4

GCFC1 Antibody
45987-50ul	SAB	50ul	EUR 224.4

GCFC1 Antibody
ABD9529	Lifescience Market	100 ug	EUR 525.6

GCFC1 Antibody
DF9529-100ul	Affinity Biosciences	100ul	EUR 280

GCFC1 Antibody
DF9529-200ul	Affinity Biosciences	200ul	EUR 350

GCFC1 Antibody
DF9529	Affinity Biosciences	100ul	EUR 280
Description: Human,Mouse

GCFC1 Antibody
E19-9529-1	EnoGene	50ug/50ul	EUR 145
Description: Available in various conjugation types.

GCFC1 Antibody
E19-9529-2	EnoGene	100ug/100ul	EUR 225
Description: Available in various conjugation types.

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV654355	ABM	1.0 ug DNA	EUR 1626

GCFC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV654359	ABM	1.0 ug DNA	EUR 1626

GCFC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV654360	ABM	1.0 ug DNA	EUR 1626

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
LV762095	ABM	1.0 ug DNA	EUR 1104

GCFC2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV762099	ABM	1.0 ug DNA	EUR 1104

GCFC2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV762100	ABM	1.0 ug DNA	EUR 1104

Human Chromosome 2 Open Reading Frame 3 (GCFC2) ELISA Kit
abx386199-96tests	Abbexa	96 tests	EUR 1093.2

GCFC1 Conjugated Antibody
C45987	SAB	100ul	EUR 476.4

GCFC1 Polyclonal Antibody
ABP58618-003ml	Abbkine	0.03ml	EUR 189.6
Description: A polyclonal antibody for detection of GCFC1 from Human, Mouse. This GCFC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GCFC1 protein

GCFC1 Polyclonal Antibody
ABP58618-01ml	Abbkine	0.1ml	EUR 346.8
Description: A polyclonal antibody for detection of GCFC1 from Human, Mouse. This GCFC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GCFC1 protein

GCFC1 Polyclonal Antibody
ABP58618-02ml	Abbkine	0.2ml	EUR 496.8
Description: A polyclonal antibody for detection of GCFC1 from Human, Mouse. This GCFC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GCFC1 protein

GCFC1 Polyclonal Antibody
ES9671-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against GCFC1 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

GCFC1 Polyclonal Antibody
ES9671-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against GCFC1 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

GCFC1 Polyclonal Antibody
E11-200725	EnoGene	100ug/100ul	EUR 225
Description: Available in various conjugation types.

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV654356	ABM	1.0 ug DNA	EUR 1626

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV654357	ABM	1.0 ug DNA	EUR 1695.6

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV654358	ABM	1.0 ug DNA	EUR 1695.6

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV762096	ABM	1.0 ug DNA	EUR 1104

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV762097	ABM	1.0 ug DNA	EUR 1173.6

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV762098	ABM	1.0 ug DNA	EUR 1173.6

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody
20-abx217653	Abbexa	EUR 510.00 EUR 410.40	100 ug 50 ug

Rabbit anti-GCFC2 Antibody
YLD3352-100ul	Shanghai YL Biotech	100 ul	EUR 320
Description: Rabbit polyclonal antibody to GCFC2

Rabbit anti-GCFC2 Antibody
YLD3352-50ul	Shanghai YL Biotech	50 ul	EUR 200
Description: Rabbit polyclonal antibody to GCFC2

Rabbit anti-GCFC2 Antibody
DL91139A-100ul	DL Develop	100 ul	EUR 299
Description: C2orf3; GCF; TCF9; GC-rich sequence DNA-binding factor 2; GC-rich sequence DNA-binding factor; Transcription factor 9; TCF-9

Rabbit anti-GCFC2 Antibody
DL91139A-50ul	DL Develop	50 ul	EUR 209.3
Description: C2orf3; GCF; TCF9; GC-rich sequence DNA-binding factor 2; GC-rich sequence DNA-binding factor; Transcription factor 9; TCF-9

GCFC1 ORF Vector (Human) (pORF)
ORF020087	ABM	1.0 ug DNA	Ask for price

Gcfc1 ORF Vector (Mouse) (pORF)
ORF045375	ABM	1.0 ug DNA	EUR 607.2

Gcfc1 3'UTR GFP Stable Cell Line
TU156964	ABM	1.0 ml	Ask for price

GCFC1 3'UTR GFP Stable Cell Line
TU058672	ABM	1.0 ml	EUR 2799.6

GCFC1 Blocking Peptide
DF9529-BP	Affbiotech	1mg	EUR 234

GCFC1-AS1 ORF Vector (Human) (pORF)
ORF020088	ABM	1.0 ug DNA	Ask for price

GCFC1 Protein Vector (Mouse) (pPM-C-HA)
PV181500	ABM	500 ng	EUR 1278

GCFC1 Protein Vector (Human) (pPM-C-HA)
PV080347	ABM	500 ng	Ask for price

GCFC1 Protein Vector (Mouse) (pPB-C-His)
PV181498	ABM	500 ng	EUR 1278

GCFC1 Protein Vector (Mouse) (pPB-N-His)
PV181499	ABM	500 ng	EUR 1278

GCFC1 Protein Vector (Mouse) (pPM-C-His)
PV181501	ABM	500 ng	EUR 1278

GCFC1 Protein Vector (Human) (pPB-C-His)
PV080345	ABM	500 ng	Ask for price

GCFC1 Protein Vector (Human) (pPB-N-His)
PV080346	ABM	500 ng	Ask for price

GCFC1 Protein Vector (Human) (pPM-C-His)
PV080348	ABM	500 ng	Ask for price

GCFC1 3'UTR Luciferase Stable Cell Line
TU008672	ABM	1.0 ml	EUR 2799.6

Gcfc1 3'UTR Luciferase Stable Cell Line
TU106964	ABM	1.0 ml	Ask for price

Gcfc1 sgRNA CRISPR Lentivector set (Mouse)
K3520201	ABM	3 x 1.0 ug	EUR 406.8

GCFC1 sgRNA CRISPR Lentivector set (Human)
K2771901	ABM	3 x 1.0 ug	EUR 406.8

Gcfc1 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K3520202	ABM	1.0 ug DNA	EUR 184.8

Gcfc1 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K3520203	ABM	1.0 ug DNA	EUR 184.8

Gcfc1 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K3520204	ABM	1.0 ug DNA	EUR 184.8

GCFC1 sgRNA CRISPR Lentivector (Human) (Target 1)
K2771902	ABM	1.0 ug DNA	EUR 184.8

GCFC1 sgRNA CRISPR Lentivector (Human) (Target 2)
K2771903	ABM	1.0 ug DNA	EUR 184.8

GCFC1 sgRNA CRISPR Lentivector (Human) (Target 3)
K2771904	ABM	1.0 ug DNA	EUR 184.8

Mouse GC- rich sequence DNA- binding factor 1, Gcfc1 ELISA KIT
ELI-37633m	Lifescience Market	96 Tests	EUR 1038

Human GC- rich sequence DNA- binding factor 1, GCFC1 ELISA KIT
ELI-08155h	Lifescience Market	96 Tests	EUR 988.8

Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K3520205	ABM	3 x 1.0 ug	EUR 451.2

GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K2771905	ABM	3 x 1.0 ug	EUR 451.2

GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
LV726545	ABM	1.0 ug DNA	Ask for price

GCFC1-AS1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV726549	ABM	1.0 ug DNA	Ask for price

Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K3520206	ABM	1.0 ug DNA	EUR 200.4

Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K3520207	ABM	1.0 ug DNA	EUR 200.4

Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K3520208	ABM	1.0 ug DNA	EUR 200.4

GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K2771906	ABM	1.0 ug DNA	EUR 200.4

GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K2771907	ABM	1.0 ug DNA	EUR 200.4

GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K2771908	ABM	1.0 ug DNA	EUR 200.4

GCFC1-AS1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV726550	ABM	1.0 ug DNA	Ask for price

GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV726546	ABM	1.0 ug DNA	Ask for price

GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV726547	ABM	1.0 ug DNA	Ask for price

GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV726548	ABM	1.0 ug DNA	Ask for price

Anti-Mouse C3 Antibody | GC3-90F-Z
GC3-90F-Z	Immunology Consultants Laboratory	0.1 mg	EUR 316
Description: Anti-Mouse C3 Antibody ; GC3-90F-Z ; Immunology Consultants Laboratory Host: Goat Format: FITC Conjugated Product Type: Primary Antibody Antibody Clonality: Polyclonal

pCMV-SPORT6-GCFC2-G181A
PVT12797	Lifescience Market	2 ug	EUR 469.2

GCF antibody
70R-36476	Fitzgerald	100 ug	EUR 392.4
Description: Rabbit polyclonal GCF antibody

GCF Antibody
34702-100ul	SAB	100ul	EUR 302.4

GCF Antibody
34702-50ul	SAB	50ul	EUR 224.4

GCF Antibody
ABD4083	Lifescience Market	100 ug	EUR 525.6

GCF Antibody
20-abx014492	Abbexa	EUR 376.80 EUR 117.60 EUR 477.60 EUR 594.00	100 ug 10 ug 200 ug 300 µg

GCF Antibody
DF4083	Affinity Biosciences	100ul	EUR 280
Description: Human,Mouse

GCF Antibody
E034702	EnoGene	100μg/100μl	EUR 255
Description: Available in various conjugation types.

GCF Antibody
E19-4083	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

GCF Antibody
E11-15891C	EnoGene	100μg	EUR 225
Description: Available in various conjugation types.

GCF Antibody
DF4083-100ul	Affinity Biosciences	100ul	EUR 280

GCF Antibody
DF4083-200ul	Affinity Biosciences	200ul	EUR 350

GCF Conjugated Antibody
C34702	SAB	100ul	EUR 476.4

Anti-Anti-SEPT5 Antibody antibody
STJ114819	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT2 Antibody antibody
STJ28365	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT7 Antibody antibody
STJ28963	St John's Laboratory	100 µl	EUR 332.4
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT3 Antibody antibody
STJ118990	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT1 antibody antibody
STJ119580	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-SEPT6 antibody antibody
STJ11100949	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody
STJ111369	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT4 Antibody antibody
STJ112276	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-SEPT7 Antibody antibody
STJ116214	St John's Laboratory	100 µl	EUR 332.4
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody
STJ117206	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT2 Antibody antibody
STJ25475	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT5 Antibody antibody
STJ25477	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody
STJ25479	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH9 Antibody antibody
STJ112609	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT11 Antibody antibody
STJ113941	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT11 Antibody antibody
STJ114081	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-MARCH8 Antibody antibody
STJ114828	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT12 Antibody antibody
STJ117759	St John's Laboratory	100 µl	EUR 332.4
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody
STJ118549	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-MARCH6 Antibody antibody
STJ118550	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-MARCH7 Antibody antibody
STJ118752	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT11 Antibody antibody
STJ111530	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-DDB1 Antibody
A00333	BosterBio	100uL	EUR 546
Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 6777.36
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 663.31
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 896.16
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3672.72
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
4-AEA465Hu	Cloud-Clone	EUR 6837.60 EUR 3613.20 EUR 896.40	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Anti-AsAb(Anti-Anti-Sperm Antibody Antibody) ELISA Kit
ELK8071-48T	ELK Biotech	48T	Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-AsAb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AsAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AsAb in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Anti-AsAb(Anti-Anti-Sperm Antibody Antibody) ELISA Kit
ELK8071-96T	ELK Biotech	96T	Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-AsAb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AsAb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AsAb in the samples is then determined by comparing the OD of the samples to the standard curve.

Anti-BOD1 antibody
STJ72155	St John's Laboratory	100 µg	EUR 430.8

Anti-BOD1L antibody
STJ73530	St John's Laboratory	100 µg	EUR 312

ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)
ELK8071	ELK Biotech	1 plate of 96 wells	EUR 518.4
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

anti- Antibody^Polyclonal antibody control antibody
LSMab09882	Lifescience Market	100 ug	EUR 525.6

Anti-2B4 Antibody
HA500180	HUABIO	100ul	EUR 189
Description: This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-2B4 Antibody
HA500263	HUABIO	100ul	EUR 189
Description: Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor . Activating function implicates association with SH2D1A and FYN . Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2. Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis . Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells. Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1. At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells. Involved in the regulation of CD8+ T-cell proliferation; expression on activated T-cells and binding to CD488 provides costimulatory-like function for neighboring T-cells. Inhibits inflammatory responses in dendritic cells (DCs) .

Anti-7B2 antibody
STJ191062	St John's Laboratory	200 µl	EUR 236.4
Description: Unconjugated Rabbit polyclonal to 7B2

Anti-4.1G Antibody
A03718	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for 4.1G Antibody (EPB41L2) detection.tested for WB in Human.

Anti-4.1G antibody
STJ91381	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to 4.1G.

Anti-4.1R antibody
STJ91382	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to 4.1R.

Anti-BOC Antibody
PA1904	BosterBio	100ug/vial	EUR 352.8

Anti-BOK antibody
STJ190601	St John's Laboratory	200 µl	EUR 236.4
Description: Unconjugated Rabbit polyclonal to BOK

Anti-BOC antibody
STJ71520	St John's Laboratory	100 µg	EUR 430.8

Anti-BOK antibody
STJ72368	St John's Laboratory	100 µg	EUR 430.8

Anti-BOC antibody
STJ192335	St John's Laboratory	200 µl	EUR 236.4
Description: Unconjugated Rabbit polyclonal to BOC

Anti-BOC antibody
STJ29254	St John's Laboratory	100 µl	EUR 332.4
Description: The protein encoded by this gene is a member of the immunoglobulin/fibronectin type III repeat family. It is a component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells, and promotes myogenic differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Anti-BoV antibody
STJ400075	St John's Laboratory	1 mg	EUR 535.2
Description: Human bocavirus (also termed HBoV) is a small non-enveloped virus with a single- stranded DNA genome. It is a member of the Parvoviridae family. HBoV has been detected worldwide in 2–20 % of all upper and lower respiratory tract infections, and has also been linked to gastroenteritis.

Anti-BOK antibody
STJ118794	St John's Laboratory	100 µl	EUR 332.4

Anti-BOC antibody
STJ115407	St John's Laboratory	100 µl	EUR 332.4
Description: The protein encoded by this gene is a member of the immunoglobulin/fibronectin type III repeat family. It is a component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells, and promotes myogenic differentiation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Anti-2B1F antibody
STJ190870	St John's Laboratory	200 µl	EUR 236.4
Description: Unconjugated Rabbit polyclonal to 2B1F

Anti-53BP1 antibody
STJ91389	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to 53BP1.

Anti-53BP1 antibody
STJ91390	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to 53BP1.

Anti-53BP1 antibody
STJ91391	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to 53BP1.

Anti-BOB1 Antibody
ER1803-97	HUABIO	100ul	EUR 189
Description: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Reduced activation of Bob1-deficient B cells following antigenic stimulation is often reported. The action of Bob1 on B cell maturation and tolerance could be mediated by effects on B cell activation, in part by regulating the expression of immunosuppressive miRNAs. Bob1 has long been considered a B cell‐specific factor that interacts with the transcription factors Oct1 and Oct2 to enhance octamer‐dependent transcription. Bob1 can directly bind to and transactivate the promoters of Bcl6 and Btla.

Anti-BOB1 antibody
STJ180315	St John's Laboratory	0.1 ml	EUR 254.4

Anti-BORA antibody
STJ190128	St John's Laboratory	200 µl	EUR 236.4
Description: Unconjugated Rabbit polyclonal to BORA

Anti-BOP1 antibody
STJ191341	St John's Laboratory	200 µl	EUR 236.4
Description: Unconjugated Rabbit polyclonal to BOP1

Anti-BOCT antibody
STJ96427	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to BOCT.

Anti-BORA antibody
STJ115786	St John's Laboratory	100 µl	EUR 332.4

anti- TH antibody
FNab08655	FN Test	100µg	EUR 702
Description: Antibody raised against TH

anti- XK antibody
FNab09543	FN Test	100µg	EUR 658.5
Description: Antibody raised against XK

anti- PR antibody
FNab09774	FN Test	100µg	EUR 658.5
Description: Antibody raised against PR

anti- T3 antibody
FNab09808	FN Test	100µg	EUR 658.5
Description: Antibody raised against T3

anti- T4 antibody
FNab09809	FN Test	100µg	EUR 658.5
Description: Antibody raised against T4

anti- TH antibody
FNab09870	FN Test	100µg	EUR 658.5
Description: Antibody raised against TH

anti- C9 antibody
FNab10107	FN Test	100µg	EUR 658.5
Description: Antibody raised against C9

anti- C9 antibody
FNab10108	FN Test	100µg	EUR 658.5
Description: Antibody raised against C9

anti- Rb antibody
FNab07140	FN Test	100µg	EUR 606.3
Description: Antibody raised against Rb

anti- ER antibody
FNab02822	FN Test	100µg	EUR 606.3
Description: Antibody raised against ER

anti- F2 antibody
FNab02920	FN Test	100µg	EUR 606.3
Description: Antibody raised against F2

anti- F5 antibody
FNab02923	FN Test	100µg	EUR 606.3
Description: Antibody raised against F5

anti- F7 antibody
FNab02924	FN Test	100µg	EUR 606.3
Description: Antibody raised against F7

anti- F9 antibody
FNab02925	FN Test	100µg	EUR 606.3
Description: Antibody raised against F9

anti- FH antibody
FNab03106	FN Test	100µg	EUR 606.3
Description: Antibody raised against FH

anti- FH antibody
FNab03107	FN Test	100µg	EUR 658.5
Description: Antibody raised against FH

anti- HP antibody
FNab03990	FN Test	100µg	EUR 606.3
Description: Antibody raised against HP

anti- C6 antibody
FNab01123	FN Test	100µg	EUR 606.3
Description: Antibody raised against C6

anti- C7 antibody
FNab01132	FN Test	100µg	EUR 606.3
Description: Antibody raised against C7

Anti-B23 antibody
PAab00759	Lifescience Market	100 ug	EUR 494.4

Anti-B19 Antibody
STJ501374	St John's Laboratory	100 µg	EUR 571.2

Anti-B23 antibody
STJ97858	St John's Laboratory	100 µl	EUR 280.8
Description: Mouse monoclonal to B23.

Anti-B23 antibody
STJ98470	St John's Laboratory	100 µl	EUR 280.8
Description: Mouse monoclonal to B23.

Anti-B23 antibody
STJ91794	St John's Laboratory	200 µl	EUR 236.4
Description: Rabbit polyclonal to B23.

anti- B23 antibody
FNab00759	FN Test	100µg	EUR 702
Description: Antibody raised against B23

anti- B23 antibody
FNab00760	FN Test	100µg	EUR 702
Description: Antibody raised against B23

Anti-3BP1 antibody
STJ191408	St John's Laboratory	200 µl	EUR 236.4
Description: Unconjugated Rabbit polyclonal to 3BP1

Anti-3BP2 antibody
STJ191409	St John's Laboratory	200 µl	EUR 236.4
Description: Unconjugated Rabbit polyclonal to 3BP2

Gemultiplexte en high-throughput labelvrije detectie van RNA/Spike  Protein /IgG/IgM-biomarkers van SARS-CoV-2-infectie met behulp van nanoplasmonische biosensoren

Om de COVID-19-uitbraak, die wordt veroorzaakt door het ernstige acute respiratoire syndroom coronavirus 2 (SARS-CoV-2), aan te pakken, is er een onvervulde behoefte aan zeer nauwkeurige diagnostische checks in alle stadia van infectie met snelle resultaten en hoge specificiteit. Hier presenteren we een labelvrije, op nanoplasmonische biosensor gebaseerde, multiplex screeningtest voor COVID-19 die 10 verschillende biomarkers kwantitatief kan detecteren (6 virale nucleïnezuurgenen, 2 spike-eiwitsubeenheden en 2 antilichamen) met een detectielimiet in de aM-reeks, allemaal binnen één biosensorplatform.

Onze nieuw ontwikkelde nanoplasmonische biosensoren vertonen een hoge specificiteit, wat van het grootste belang is om valse reacties te voorkomen. Als proof of idea laten we zien dat onze detectiebenadering het potentieel heeft om zowel IgG- als IgM- antilichamen rechtstreeks uit COVID-19-positieve patiëntplasmamonsters te kwantificeren in een enkele instrumentrun, wat de hoge doorvoercapaciteit van onze detectiebenadering aantoont. Het belangrijkste is dat onze take a look at ontvangende operationele kenmerken biedt, gebieden onder de curve van respectievelijk 0,997 en 0,999 voor IgG en IgM. De berekende  p- waarde bepaald met de Mann-Whitney niet-parametrische take a look at is <0,0001 voor beide antilichamen wanneer de take a look at van COVID-19-positieve patiënten ( n = 80) wordt vergeleken met die van gezonde personen ( n  = 72).

Bovendien geeft de screeningstest een berekende sensitiviteit (true constructive fee) van 100% (80/80), een specificiteit (true negatieve fee) >96% (77/80) , een positief voorspellende waarde van 98% bij een prevalentie van 5% en een negatief voorspellende waarde van 100% bij een prevalentie van 5%. Wij zijn van mening dat onze zeer gevoelige, multiplex, high-throughput testbenadering potentiële toepassingen heeft in de COVID-19-diagnostiek , met title bij het bepalen van virusprogressie en infectie-ernst voor clinici voor een geschikte behandeling, en ook een zeer effectieve diagnostische take a look at zal blijken te zijn wanneer toegepast op ziekten buiten de COVID-19-pandemie.

Nauwkeurigheid van alternatieve niet-polariseerbare krachtvelden voor de bepaling van  eiwit- ligandbindingsaffiniteiten gedomineerd door kation-π-interacties

Het aanpassen van paarspecifieke Lennard-Jones-parameters by way of de niet-gebonden FIX (NBFIX)-functie van het CHARMM36-krachtveld is kosteneffectief gebleken voor het verbeteren van de beschrijving van kation-π-interacties in biologische objecten door middel van paarsgewijs additieve potentiële energiefuncties. Hier worden twee units nieuw geoptimaliseerde CHARMM36-krachtveldparameters, waaronder NBFIX-correcties, bedacht CHARMM36m-NBF en CHARMM36-WYF, en de originele krachtvelden, namelijk CHARMM36m en Amber ff14SB, gebruikt om de standaard bindingsvrije energieën van zeven eiwit- ligandcomplexen die kation-π-interacties bevatten.

Vergeleken met nauwkeurige experimentele metingen, geven onze resultaten aan dat de ongecorrigeerde, originele krachtvelden de bindingsvrije energieën vital onderschatten, met een gemiddelde fout van 5,Three kcal/mol, terwijl de gemiddelde fouten van CHARMM36m-NBF en CHARMM36-WYF 0,eight en 2,1 bedragen. respectievelijk kcal/mol. De huidige studie toont overtuigend aan dat het gebruik van gewijzigde parameters samen met NBFIX-correcties de nauwkeurigheid van de standaard bindingsvrije energie van eiwit-ligandcomplexen die worden gedomineerd door kation-π-interacties, met title met CHARMM36m-NBF, dramatisch verhoogt.

Gebruikmakend van door tijd opgeloste, door  proteïne geïnduceerde fluorescentieverbetering om stabiele lokale conformaties te identificeren, één α-synucleïne-monomeer per keer

Het gebruik van spectroscopische linialen om meerdere conformaties van afzonderlijke biomoleculen en hun dynamiek te volgen, heeft een revolutie teweeggebracht in het begrip van structurele dynamiek en zijn bijdragen aan de biologie. Terwijl de op FRET gebaseerde liniaal rapporteert over afstanden tussen kleurstoffen in het bereik van 3-10 nm, rapporteren andere spectroscopische technieken, zoals eiwit-geïnduceerde fluorescentieverbetering (PIFE), over de nabijheid tussen een kleurstof en een eiwitoppervlak in de kortere 0 -Three nm bereik. Ongeacht de gekozen methode, het gebruik ervan bij het één voor één meten van vrij diffunderende biomoleculen haalt histogrammen op van de experimentele parameter die afzonderlijke centraal verdeelde subpopulaties van biomoleculen oplevert, waarbij elke subpopulatie vertegenwoordigtofwel een enkele conformatie die binnen milliseconden onveranderd bleef, of meerdere conformaties die veel sneller dan milliseconden in elkaar overgaan, en dus een uitgemiddelde subpopulatie.

In FRET met één molecuul, waar de gerapporteerde parameter in histogrammen de FRET-efficiëntie tussen kleurstoffen is, werd eerder gerapporteerd dat een intrinsiek ongeordend eiwit, zoals het α-synucleïne-monomeer in buffer, een enkele gemiddelde subpopulatie van meerdere conformaties die snel in elkaar overgaan. Hoewel deze eerdere bevindingen afhankelijk zijn van het bereik van 3-10 nm van de FRET-gebaseerde liniaal, hebben we geprobeerd dit eiwit op de proef te stellen met behulp van PIFE met één molecuul, waarbij we de fluorescentielevensduur van plaatsspecifieke sCy3-gelabelde α-Synuclein volgen. eiwitten één voor één. Interessant is dat met behulp van deze spectroscopische naderingssensor met een kortere afstand, sCy3-gelabeld -Synucleïne verschillende subpopulaties met een duidelijk verschillende gemiddelde levensduur vertoont die in 10-100 ms in elkaar overgaan.Deze resultaten laten zien dat, hoewel α-synucleïne wereldwijd ontregeld kan zijn, het toch stabiele lokale structuren bereikt. Samenvattend, in dit werk benadrukken we het voordeel van het gebruik van verschillende spectroscopische nabijheidssensoren die lokale of globale structurele veranderingen één biomolecuul tegelijk volgen.