unitech-europe

Nauwkeurigheid van alternatieve niet-polariseerbare krachtvelden

SPC1 regelt de signaal-peptidase bemiddelde verwerken van membraan  proteïnen

Signaalpeptidase (SPase) splitst de signaalsequenties (SS’s ) van secretoire voorlopers. Het bevat een evolutionair geconserveerde membraaneiwitsubeenheid, Spcl, die niet nodig is voor de katalytische activiteit van SPase, en de rol ervan blijft onbekend. In de huidige studie hebben we de functie van gist Spc1 onderzocht. Eerst hebben we een in vivo SPase-splitsingstest opgezet met behulp van secretoire eiwit-CPY- varianten met SS’s gemodificeerd in de n- en h-regio’s.

Bij het vergelijken van de SS-splitsingsefficiëntie van deze varianten in cellen met of zonder Spcl, ontdekten we dat signaalverankerde sequenties vatbaarder werden voor splitsing door SPase zonder Spcl. Verder was SPase-gemedieerde verwerking van modelmembraaneiwitten verbeterd in de afwezigheid van maar verminderd bij overexpressie van Spcl. Spcl werd geco-immunoprecipiteerd met eiwitten die niet-gesplitste signaalverankerde of transmembraan (TM) segmenten droegen.

Samengevat suggereren deze resultaten dat Spc1 TM-segmenten beschermt tegen SPase-actie, waardoor de substraatselectie voor SPase wordt verscherpt en fungeert als een negatieve regulator voor de SPase-gemedieerde verwerking van membraaneiwitten .

Interleukine-10 speelt een belangrijke rol in pasgeboren ratten met Hypoxic-ischemie geassocieerd met B-cel lymfoom 2 en endoplasmatisch reticulum  Protein  29

 

Interleukine 10 (IL-10) is een synthetische remmer van humane cytokines met immunomodulerende en ontstekingsremmende effecten. Deze studie was bedoeld om de expressievariatie van IL-10 op meerdere plaatsen, waaronder cortex, hippocampus en longweefsels van neonatale hypoxisch-ischemische (HI) ratten te onderzoeken en de cruciale rol van IL-10 bij het verlichten van HI-hersenschade te onderzoeken. In deze studie werden neonatale Sprague-Dawley-ratten onderworpen aan de ligatie van de rechter gemeenschappelijke halsslagader, gevolgd door 2 uur hypoxie.

De expressie van IL-10 in de cortex, hippocampus en longweefsels werd gemeten met immunohistochemie, realtime kwantitatieve polymerasekettingreactie (RT-qPCR) en western blot (WB). Dubbele kleuring met immunofluorescentie werd uitgevoerd om de lokalisatie van IL-10 in neuronen en astrocyten te observeren . Bovendien werden niet-targeting en concentrating on IL-10 siRNA-lentivirusvectoren geïnjecteerd in respectievelijk de ratten van de negatieve controle (NC) en IL-10-groep, en de mRNA-niveaus van B-cellymfoom 2 (Bcl-2) en endoplasmatische reticulum eiwit 29 (ERp29) werden gedetecteerd door RT-qPCR na IL-10 stilte.

De resultaten toonden aan dat de IL-10-expressie aanzienlijk was verhoogd nadat HI en IL-10 waren gecolokaliseerd met neuronen en astrocyten die ernstig waren beschadigd door HI-belediging. Bovendien waren Bcl-2 en ERp29 opmerkelijk verlaagd na IL-10-mRNA-interferentie in vergelijking met de NC-groep. Onze bevindingen onthulden dat IL-10 zijn anti-apoptotische en neuroprotectieve effecten uitoefende door de expressie van Bcl-2 en ERp29 te reguleren, wat aangeeft dat IL-10 een veelbelovend molecuuldoelwit kan zijn voor HIE-behandeling.

unitech-europe
unitech-europe

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody

20-abx323787
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  • 100 ug
  • 50 ug

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody

abx330966-100ul 100 ul
EUR 510

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody

20-abx008656
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  • 100 ul
  • 200 ul
  • 30 ul

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody

abx323787-100g 100 µg
EUR 250

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody

abx323787-50g 50 µg
EUR 187.5

ARP58089_P050 - GCFC2 Antibody - N-terminal region (ARP58089_P050)

ARP58089_P050 100ul
EUR 389

GCFC2 siRNA

20-abx917674
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  • 15 nmol
  • 30 nmol

GCFC2 siRNA

20-abx917675
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  • 15 nmol
  • 30 nmol

GCFC2 Blocking Peptide

20-abx063333
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  • 1 mg
  • 5 mg

Human GCFC2 shRNA Plasmid

20-abx954755
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  • 150 µg
  • 300 µg

Mouse GCFC2 shRNA Plasmid

20-abx983608
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  • 150 µg
  • 300 µg

GCFC2 Recombinant Protein (Rat)

RP202352 100 ug Ask for price

GCFC2 Recombinant Protein (Human)

RP060264 100 ug Ask for price

Gcfc2 ORF Vector (Rat) (pORF)

ORF067452 1.0 ug DNA
EUR 607.2

GCFC2 ORF Vector (Human) (pORF)

ORF020089 1.0 ug DNA Ask for price

Human GCFC2 knockout cell line

ABC-KH5954 1 vial Ask for price
Description: Human GCFC2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human GCFC2 knockdown cell line

ABC-KD5954 1 vial Ask for price
Description: Human GCFC2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human GCFC2 Protein Lysate 20ug

IHUGCFC2PLLY20UG each
EUR 213
Description: Human GCFC2 Protein Lysate 20ug

OCOA13748-20UG - GCFC2 Protein Lysate

OCOA13748-20UG 20ug
EUR 169

GCFC2 (untagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 2

SC331408 10 µg Ask for price

GCFC2 (untagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 3

SC331409 10 µg Ask for price

GCFC2 (GFP-tagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 2

RG234563 10 µg Ask for price

GCFC2 (GFP-tagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 3

RG233464 10 µg Ask for price

GCFC2 Protein Vector (Rat) (pPM-C-HA)

PV269808 500 ng
EUR 1399.2

GCFC2 Protein Vector (Rat) (pPB-C-His)

PV269806 500 ng
EUR 1399.2

GCFC2 Protein Vector (Rat) (pPB-N-His)

PV269807 500 ng
EUR 1399.2

GCFC2 Protein Vector (Rat) (pPM-C-His)

PV269809 500 ng
EUR 1399.2

GCFC2 (Myc-DDK tagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 2

RC234563 10 µg Ask for price

GCFC2 (Myc-DDK tagged) - Homo sapiens GC-rich sequence DNA-binding factor 2 (GCFC2), transcript variant 3

RC233464 10 µg Ask for price

GCFC2 Protein Vector (Human) (pPM-C-HA)

PV080355 500 ng Ask for price

GCFC2 Protein Vector (Human) (pPB-C-His)

PV080353 500 ng Ask for price

GCFC2 Protein Vector (Human) (pPB-N-His)

PV080354 500 ng Ask for price

GCFC2 Protein Vector (Human) (pPM-C-His)

PV080356 500 ng Ask for price

AAP58089-100UG - GCFC2 Peptide - N-terminal region

AAP58089-100UG 100ug
EUR 99

AAP58090-100UG - GCFC2 Peptide - N-terminal region

AAP58090-100UG 100ug
EUR 99

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV654355 1.0 ug DNA
EUR 1626

GCFC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV654359 1.0 ug DNA
EUR 1626

GCFC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV654360 1.0 ug DNA
EUR 1626

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

LV762095 1.0 ug DNA
EUR 1104

GCFC2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV762099 1.0 ug DNA
EUR 1104

GCFC2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)

LV762100 1.0 ug DNA
EUR 1104

GCFC1 Antibody

E19-9529-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

GCFC1 Antibody

E19-9529-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

GCFC1 Antibody

DF9529 200ul
EUR 420

GCFC1 Antibody

DF9529-100ul 100ul
EUR 280

GCFC1 Antibody

DF9529-200ul 200ul
EUR 350

GCFC1 Antibody

45987-100ul 100ul
EUR 302.4

GCFC1 Antibody

45987-50ul 50ul
EUR 224.4

GCFC1 Antibody

ABD9529 100 ug
EUR 525.6

Gcfc2 (untagged) - Mouse expressed sequence AW146020 (AW146020), (10ug)

MC221471 10 µg Ask for price

Gcfc2 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

SR419974 2 nmol Ask for price

GCFC2 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

SR304747 2 nmol Ask for price

Human Chromosome 2 Open Reading Frame 3 (GCFC2) ELISA Kit

abx386199-96tests 96 tests
EUR 1093.2

Gcfc2 (Myc-DDK-tagged) - Mouse expressed sequence AW146020 (AW146020)

MR219015 10 µg Ask for price

Gcfc2 (GFP-tagged) - Mouse expressed sequence AW146020 (AW146020), (10ug)

MG219015 10 µg Ask for price

Mouse GC-rich sequence DNA-binding factor 2 (GCFC2) ELISA Kit

abx524991-96tests 96 tests
EUR 687.5

Gcfc2 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector

TL708694 5 µg/vial Ask for price

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV654356 1.0 ug DNA
EUR 1626

GCFC1 Polyclonal Antibody

E11-200725 100ug/100ul
EUR 225
Description: Available in various conjugation types.

GCFC1 Conjugated Antibody

C45987 100ul
EUR 476.4

GCFC1 Polyclonal Antibody

ABP58618-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GCFC1 from Human, Mouse. This GCFC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GCFC1 protein

GCFC1 Polyclonal Antibody

ABP58618-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GCFC1 from Human, Mouse. This GCFC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GCFC1 protein

GCFC1 Polyclonal Antibody

ABP58618-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GCFC1 from Human, Mouse. This GCFC1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human GCFC1 protein

GCFC1 Polyclonal Antibody

E44H08332 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Gcfc2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector

TL508464 5 µg/vial Ask for price

GCFC2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

TL314319 5 µg/vial Ask for price

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV654357 1.0 ug DNA
EUR 1695.6

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV654358 1.0 ug DNA
EUR 1695.6

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV762096 1.0 ug DNA
EUR 1104

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV762097 1.0 ug DNA
EUR 1173.6

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV762098 1.0 ug DNA
EUR 1173.6

Gcfc2 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vectorr

TR708694 5 µg/vial Ask for price

Gcfc2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vectorr

TR508464 5 µg/vial Ask for price

GCFC2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector

TR314319 5 µg/vial Ask for price

GCFC1 Rabbit Polyclonal Antibody

ES9671-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against GCFC1 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

GCFC1 Rabbit Polyclonal Antibody

ES9671-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against GCFC1 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

ARP83373_P050 - GCFC1 Antibody - middle region (ARP83373_P050)

ARP83373_P050 100ul
EUR 389

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody

20-abx217653
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  • 100 ug
  • 50 ug

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody

abx217653-100tests 100 tests Ask for price

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody

abx217653-200tests 200 tests Ask for price

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody

abx217653-20tests 20 tests
EUR 87.5

GC-Rich Sequence DNA-Binding Factor 1 (GCFC1) Antibody

abx375543-96tests 96 tests
EUR 337.5

Lenti ORF clone of Gcfc2 (mGFP-tagged) - Mouse expressed sequence AW146020 (AW146020)

MR219015L4 10 µg Ask for price

GCFC2 (untagged)-Human chromosome 2 open reading frame 3 (C2orf3), transcript variant 1

SC127315 10 µg Ask for price

Lenti ORF clone of Gcfc2 (Myc-DDK-tagged) - Mouse expressed sequence AW146020 (AW146020)

MR219015L3 10 µg Ask for price

Rabbit anti-GCFC2 Antibody

DL91139A-100ul 100 ul
EUR 299
Description: C2orf3; GCF; TCF9; GC-rich sequence DNA-binding factor 2; GC-rich sequence DNA-binding factor; Transcription factor 9; TCF-9

Rabbit anti-GCFC2 Antibody

DL91139A-50ul 50 ul
EUR 209.3
Description: C2orf3; GCF; TCF9; GC-rich sequence DNA-binding factor 2; GC-rich sequence DNA-binding factor; Transcription factor 9; TCF-9

Rabbit anti-GCFC2 Antibody

YLD3352-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to GCFC2

Rabbit anti-GCFC2 Antibody

YLD3352-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to GCFC2

GCFC2 (GFP-tagged) - Human chromosome 2 open reading frame 3 (C2orf3), transcript variant 1

RG209540 10 µg Ask for price

GCFC2 (Myc-DDK-tagged)-Human chromosome 2 open reading frame 3 (C2orf3), transcript variant 1

RC209540 10 µg Ask for price

Lenti ORF particles, Gcfc2 (GFP-tagged) - Mouse expressed sequence AW146020 (AW146020), 200ul, >10^7 TU/mL

MR219015L4V 200 µl Ask for price

Lenti ORF particles, Gcfc2 (Myc-DDK-tagged) - Mouse expressed sequence AW146020 (AW146020), 200ul, >10^7 TU/mL

MR219015L3V 200 µl Ask for price

Lenti ORF particles, GCFC2 (mGFP-tagged) - Human chromosome 2 open reading frame 3 (C2orf3), transcript variant 1, 200ul, >10^7 TU/mL

RC209540L4V 200 µl Ask for price

Lenti ORF particles, GCFC2 (Myc-DDK tagged) - Human chromosome 2 open reading frame 3 (C2orf3), transcript variant 1, 200ul, >10^7 TU/mL

RC209540L3V 200 µl Ask for price

Gcfc2 (untagged ORF) - Rat similar to chromosome 2 open reading frame 3, transcription factor 9 (binds GC-rich sequences) (RGD1304792), (10 ug)

RN212246 10 µg Ask for price

Gcfc2 (Myc-DDK-tagged ORF) - Rat similar to chromosome 2 open reading frame 3, transcription factor 9 (binds GC-rich sequences) (RGD1304792), (10

RR212246 10 µg Ask for price

Lenti ORF particles, Gcfc2 (GFP-tagged ORF) - Rat similar to chromosome 2 open reading frame 3; transcription factor 9 (binds GC-rich sequences)

RR212246L4V 200 µl Ask for price

Lenti ORF clone of Gcfc2 (mGFP-tagged ORF) - Rat similar to chromosome 2 open reading frame 3; transcription factor 9 (binds GC-rich sequences) (

RR212246L4 10 µg Ask for price

Lenti ORF clone of Gcfc2 (Myc-DDK-tagged ORF) - Rat similar to chromosome 2 open reading frame 3; transcription factor 9 (binds GC-rich sequences

RR212246L3 10 µg Ask for price

Lenti ORF particles, Gcfc2 (Myc-DDK-tagged ORF) - Rat similar to chromosome 2 open reading frame 3; transcription factor 9 (binds GC-rich sequenc

RR212246L3V 200 µl Ask for price

GCFC1 ORF Vector (Human) (pORF)

ORF020087 1.0 ug DNA Ask for price

Gcfc1 ORF Vector (Mouse) (pORF)

ORF045375 1.0 ug DNA
EUR 607.2

Gcfc1 3'UTR GFP Stable Cell Line

TU156964 1.0 ml Ask for price

GCFC1 3'UTR GFP Stable Cell Line

TU058672 1.0 ml
EUR 2799.6

GCFC1 Blocking Peptide

DF9529-BP 1mg
EUR 234

GCFC1-AS1 ORF Vector (Human) (pORF)

ORF020088 1.0 ug DNA Ask for price

GCFC1 Protein Vector (Human) (pPM-C-HA)

PV080347 500 ng Ask for price

GCFC1 Protein Vector (Mouse) (pPM-C-HA)

PV181500 500 ng
EUR 1278

GCFC1 Protein Vector (Human) (pPB-C-His)

PV080345 500 ng Ask for price

GCFC1 Protein Vector (Human) (pPB-N-His)

PV080346 500 ng Ask for price

GCFC1 Protein Vector (Human) (pPM-C-His)

PV080348 500 ng Ask for price

GCFC1 Protein Vector (Mouse) (pPB-C-His)

PV181498 500 ng
EUR 1278

GCFC1 Protein Vector (Mouse) (pPB-N-His)

PV181499 500 ng
EUR 1278

GCFC1 Protein Vector (Mouse) (pPM-C-His)

PV181501 500 ng
EUR 1278

Gcfc1 3'UTR Luciferase Stable Cell Line

TU106964 1.0 ml Ask for price

GCFC1 3'UTR Luciferase Stable Cell Line

TU008672 1.0 ml
EUR 2799.6

Gcfc1 sgRNA CRISPR Lentivector set (Mouse)

K3520201 3 x 1.0 ug
EUR 406.8

GCFC1 sgRNA CRISPR Lentivector set (Human)

K2771901 3 x 1.0 ug
EUR 406.8

Gcfc1 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3520202 1.0 ug DNA
EUR 184.8

Gcfc1 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3520203 1.0 ug DNA
EUR 184.8

Gcfc1 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3520204 1.0 ug DNA
EUR 184.8

GCFC1 sgRNA CRISPR Lentivector (Human) (Target 1)

K2771902 1.0 ug DNA
EUR 184.8

GCFC1 sgRNA CRISPR Lentivector (Human) (Target 2)

K2771903 1.0 ug DNA
EUR 184.8

GCFC1 sgRNA CRISPR Lentivector (Human) (Target 3)

K2771904 1.0 ug DNA
EUR 184.8

AAP83373-100UG - GCFC1 Peptide - middle region

AAP83373-100UG 100ug
EUR 99

Mouse GC- rich sequence DNA- binding factor 1, Gcfc1 ELISA KIT

ELI-37633m 96 Tests
EUR 1038

Human GC- rich sequence DNA- binding factor 1, GCFC1 ELISA KIT

ELI-08155h 96 Tests
EUR 988.8

Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3520205 3 x 1.0 ug
EUR 451.2

GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K2771905 3 x 1.0 ug
EUR 451.2

Lenti ORF particles, Paxbp1 (GFP-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1), 200ul, >10^7 TU/mL

MR222463L4V 200 µl Ask for price

Mouse GC-rich sequence DNA-binding factor 1 (GCFC1) ELISA Kit

abx524989-96tests 96 tests
EUR 687.5

GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

LV726545 1.0 ug DNA Ask for price

GCFC1-AS1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV726549 1.0 ug DNA Ask for price

Lenti ORF particles, Paxbp1 (Myc-DDK-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1), 200ul, >10^7 TU/mL

MR222463L3V 200 µl Ask for price

Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3520206 1.0 ug DNA
EUR 200.4

Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3520207 1.0 ug DNA
EUR 200.4

Gcfc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3520208 1.0 ug DNA
EUR 200.4

GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K2771906 1.0 ug DNA
EUR 200.4

GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K2771907 1.0 ug DNA
EUR 200.4

GCFC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K2771908 1.0 ug DNA
EUR 200.4

GCFC1-AS1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)

LV726550 1.0 ug DNA Ask for price

GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV726546 1.0 ug DNA Ask for price

Paxbp1 (untagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1), (10ug)

MC222591 10 µg Ask for price

Paxbp1 (GFP-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1), (10ug)

MG222463 10 µg Ask for price

GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV726547 1.0 ug DNA Ask for price

GCFC1-AS1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV726548 1.0 ug DNA Ask for price

Paxbp1 (Myc-DDK-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1)

MR222463 10 µg Ask for price

Lenti ORF clone of Paxbp1 (mGFP-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1)

MR222463L4 10 µg Ask for price

Lenti ORF clone of Paxbp1 (Myc-DDK-tagged) - Mouse GC-rich sequence DNA-binding factor 1 (Gcfc1)

MR222463L3 10 µg Ask for price

PAXBP1 (untagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2

SC314763 10 µg Ask for price

PAXBP1 (untagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

SC314917 10 µg Ask for price

PAXBP1 (GFP-tagged) - Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

RG224365 10 µg Ask for price

PAXBP1 (GFP-tagged) - Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2

RG224419 10 µg Ask for price

PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

RC224365 10 µg Ask for price

PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2

RC224419 10 µg Ask for price

Lenti-ORF clone of PAXBP1 (mGFP-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

RC224365L4 10 µg Ask for price

Lenti-ORF clone of PAXBP1 (mGFP-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2

RC224419L4 10 µg Ask for price

Lenti-ORF clone of PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1

RC224365L3 10 µg Ask for price

Lenti ORF particles, PAXBP1 (mGFP-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1, 200ul, >10^7 TU/mL

RC224365L4V 200 µl Ask for price

Lenti-ORF clone of PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2

RC224419L3 10 µg Ask for price

Lenti ORF particles, PAXBP1 (mGFP-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2, 200ul, >10^7 TU/mL

RC224419L4V 200 µl Ask for price

Lenti ORF particles, PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 1, 200ul, >10^7 TU/mL

RC224365L3V 200 µl Ask for price

Lenti ORF particles, PAXBP1 (Myc-DDK-tagged)-Human GC-rich sequence DNA-binding factor 1 (GCFC1), transcript variant 2, 200ul, >10^7 TU/mL

RC224419L3V 200 µl Ask for price

Anti-Mouse C3 Antibody | GC3-90F-Z

GC3-90F-Z 0.1 mg
EUR 316
Description:

Anti-Mouse C3 Antibody ; GC3-90F-Z ; Immunology Consultants Laboratory

Host: Goat

Format: FITC Conjugated

Product Type: Primary Antibody

Antibody Clonality: Polyclonal

pCMV-SPORT6-GCFC2-G181A

PVT12797 2 ug
EUR 469.2

GCF Antibody

34702 100ul
EUR 319

GCF Antibody

34702-100ul 100ul
EUR 302.4

GCF Antibody

34702-50ul 50ul
EUR 224.4

GCF Antibody

E034702 100μg/100μl
EUR 255
Description: Available in various conjugation types.

GCF Antibody

E19-4083 100μg/100μl
EUR 225
Description: Available in various conjugation types.

GCF Antibody

E11-15891C 100μg
EUR 225
Description: Available in various conjugation types.

GCF Antibody

DF4083 200ul
EUR 420

GCF Antibody

DF4083-100ul 100ul
EUR 280

GCF Antibody

DF4083-200ul 200ul
EUR 350

GCF antibody

70R-36476 100 ug
EUR 294
Description: Rabbit polyclonal GCF antibody

GCF Antibody

ABD4083 100 ug
EUR 525.6

GCF Antibody

8C15891 50ug
EUR 368
Description: GCF Antibody

GCF Antibody

20-abx014492
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  • Ask for price
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  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

GCF Antibody

abx014492-100g 100 µg
EUR 43.75

Anti-Anti-SEPT5 Antibody antibody

STJ114819 100 µl
EUR 332.4
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT7 Antibody antibody

STJ116214 100 µl
EUR 332.4
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody

STJ117206 100 µl
EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT1 antibody antibody

STJ119580 100 µl
EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-SEPT3 Antibody antibody

STJ118990 100 µl
EUR 332.4

Anti-Anti-SEPT4 Antibody antibody

STJ112276 100 µl
EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-SEPT2 Antibody antibody

STJ25475 100 µl
EUR 332.4

Anti-Anti-SEPT5 Antibody antibody

STJ25477 100 µl
EUR 332.4
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody

STJ25479 100 µl
EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT6 antibody antibody

STJ11100949 100 µl
EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Gemultiplexte en high-throughput labelvrije detectie van RNA/Spike  Protein /IgG/IgM-biomarkers van SARS-CoV-2-infectie met behulp van nanoplasmonische biosensoren

Om de COVID-19-uitbraak, die wordt veroorzaakt door het ernstige acute respiratoire syndroom coronavirus 2 (SARS-CoV-2), aan te pakken, is er een onvervulde behoefte aan zeer nauwkeurige diagnostische checks in alle stadia van infectie met snelle resultaten en hoge specificiteit. Hier presenteren we een labelvrije, op nanoplasmonische biosensor gebaseerde, multiplex screeningtest voor COVID-19 die 10 verschillende biomarkers kwantitatief kan detecteren (6 virale nucleïnezuurgenen, 2 spike-eiwitsubeenheden en 2 antilichamen) met een detectielimiet in de aM-reeks, allemaal binnen één biosensorplatform.

Onze nieuw ontwikkelde nanoplasmonische biosensoren vertonen een hoge specificiteit, wat van het grootste belang is om valse reacties te voorkomen. Als proof of idea laten we zien dat onze detectiebenadering het potentieel heeft om zowel IgG- als IgM- antilichamen rechtstreeks uit COVID-19-positieve patiëntplasmamonsters te kwantificeren in een enkele instrumentrun, wat de hoge doorvoercapaciteit van onze detectiebenadering aantoont. Het belangrijkste is dat onze take a look at ontvangende operationele kenmerken biedt, gebieden onder de curve van respectievelijk 0,997 en 0,999 voor IgG en IgM. De berekende  p- waarde bepaald met de Mann-Whitney niet-parametrische take a look at is <0,0001 voor beide antilichamen wanneer de take a look at van COVID-19-positieve patiënten ( n = 80) wordt vergeleken met die van gezonde personen ( n  = 72).

Bovendien geeft de screeningstest een berekende sensitiviteit (true constructive fee) van 100% (80/80), een specificiteit (true negatieve fee) >96% (77/80) , een positief voorspellende waarde van 98% bij een prevalentie van 5% en een negatief voorspellende waarde van 100% bij een prevalentie van 5%. Wij zijn van mening dat onze zeer gevoelige, multiplex, high-throughput testbenadering potentiële toepassingen heeft in de COVID-19-diagnostiek , met title bij het bepalen van virusprogressie en infectie-ernst voor clinici voor een geschikte behandeling, en ook een zeer effectieve diagnostische take a look at zal blijken te zijn wanneer toegepast op ziekten buiten de COVID-19-pandemie.

Nauwkeurigheid van alternatieve niet-polariseerbare krachtvelden voor de bepaling van  eiwit- ligandbindingsaffiniteiten gedomineerd door kation-π-interacties

Het aanpassen van paarspecifieke Lennard-Jones-parameters by way of de niet-gebonden FIX (NBFIX)-functie van het CHARMM36-krachtveld is kosteneffectief gebleken voor het verbeteren van de beschrijving van kation-π-interacties in biologische objecten door middel van paarsgewijs additieve potentiële energiefuncties. Hier worden twee units nieuw geoptimaliseerde CHARMM36-krachtveldparameters, waaronder NBFIX-correcties, bedacht CHARMM36m-NBF en CHARMM36-WYF, en de originele krachtvelden, namelijk CHARMM36m en Amber ff14SB, gebruikt om de standaard bindingsvrije energieën van zeven eiwit- ligandcomplexen die kation-π-interacties bevatten.

Vergeleken met nauwkeurige experimentele metingen, geven onze resultaten aan dat de ongecorrigeerde, originele krachtvelden de bindingsvrije energieën vital onderschatten, met een gemiddelde fout van 5,Three kcal/mol, terwijl de gemiddelde fouten van CHARMM36m-NBF en CHARMM36-WYF 0,eight en 2,1 bedragen. respectievelijk kcal/mol. De huidige studie toont overtuigend aan dat het gebruik van gewijzigde parameters samen met NBFIX-correcties de nauwkeurigheid van de standaard bindingsvrije energie van eiwit-ligandcomplexen die worden gedomineerd door kation-π-interacties, met title met CHARMM36m-NBF, dramatisch verhoogt.

Gebruikmakend van door tijd opgeloste, door  proteïne geïnduceerde fluorescentieverbetering om stabiele lokale conformaties te identificeren, één α-synucleïne-monomeer per keer

Het gebruik van spectroscopische linialen om meerdere conformaties van afzonderlijke biomoleculen en hun dynamiek te volgen, heeft een revolutie teweeggebracht in het begrip van structurele dynamiek en zijn bijdragen aan de biologie. Terwijl de op FRET gebaseerde liniaal rapporteert over afstanden tussen kleurstoffen in het bereik van 3-10 nm, rapporteren andere spectroscopische technieken, zoals eiwit-geïnduceerde fluorescentieverbetering (PIFE), over de nabijheid tussen een kleurstof en een eiwitoppervlak in de kortere 0 -Three nm bereik. Ongeacht de gekozen methode, het gebruik ervan bij het één voor één meten van vrij diffunderende biomoleculen haalt histogrammen op van de experimentele parameter die afzonderlijke centraal verdeelde subpopulaties van biomoleculen oplevert, waarbij elke subpopulatie vertegenwoordigtofwel een enkele conformatie die binnen milliseconden onveranderd bleef, of meerdere conformaties die veel sneller dan milliseconden in elkaar overgaan, en dus een uitgemiddelde subpopulatie.

In FRET met één molecuul, waar de gerapporteerde parameter in histogrammen de FRET-efficiëntie tussen kleurstoffen is, werd eerder gerapporteerd dat een intrinsiek ongeordend eiwit, zoals het α-synucleïne-monomeer in buffer, een enkele gemiddelde subpopulatie van meerdere conformaties die snel in elkaar overgaan. Hoewel deze eerdere bevindingen afhankelijk zijn van het bereik van 3-10 nm van de FRET-gebaseerde liniaal, hebben we geprobeerd dit eiwit op de proef te stellen met behulp van PIFE met één molecuul, waarbij we de fluorescentielevensduur van plaatsspecifieke sCy3-gelabelde α-Synuclein volgen. eiwitten één voor één. Interessant is dat met behulp van deze spectroscopische naderingssensor met een kortere afstand, sCy3-gelabeld -Synucleïne verschillende subpopulaties met een duidelijk verschillende gemiddelde levensduur vertoont die in 10-100 ms in elkaar overgaan.Deze resultaten laten zien dat, hoewel α-synucleïne wereldwijd ontregeld kan zijn, het toch stabiele lokale structuren bereikt. Samenvattend, in dit werk benadrukken we het voordeel van het gebruik van verschillende spectroscopische nabijheidssensoren die lokale of globale structurele veranderingen één biomolecuul tegelijk volgen.

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