SPC1 regelt de signaal-peptidase bemiddelde verwerken van membraan  proteïnen

Signaalpeptidase (SPase) splitst de signaalsequenties (SS’s ) van secretoire voorlopers. Het bevat een evolutionair geconserveerde membraaneiwitsubeenheid, Spcl, die niet nodig is voor de katalytische activiteit van SPase, en de rol ervan blijft onbekend. In de huidige studie hebben we de functie van gist Spc1 onderzocht. Eerst hebben we een in vivo SPase-splitsingstest opgezet met behulp van secretoire eiwit-CPY- varianten met SS’s gemodificeerd in de n- en h-regio’s.

Bij het vergelijken van de SS-splitsingsefficiëntie van deze varianten in cellen met of zonder Spcl, ontdekten we dat signaalverankerde sequenties vatbaarder werden voor splitsing door SPase zonder Spcl. Verder was SPase-gemedieerde verwerking van modelmembraaneiwitten verbeterd in de afwezigheid van maar verminderd bij overexpressie van Spcl. Spcl werd geco-immunoprecipiteerd met eiwitten die niet-gesplitste signaalverankerde of transmembraan (TM) segmenten droegen.

Samengevat suggereren deze resultaten dat Spc1 TM-segmenten beschermt tegen SPase-actie, waardoor de substraatselectie voor SPase wordt verscherpt en fungeert als een negatieve regulator voor de SPase-gemedieerde verwerking van membraaneiwitten .

Interleukine-10 speelt een belangrijke rol in pasgeboren ratten met Hypoxic-ischemie geassocieerd met B-cel lymfoom 2 en endoplasmatisch reticulum  Protein  29

 

Interleukine 10 (IL-10) is een synthetische remmer van humane cytokines met immunomodulerende en ontstekingsremmende effecten. Deze studie was bedoeld om de expressievariatie van IL-10 op meerdere plaatsen, waaronder cortex, hippocampus en longweefsels van neonatale hypoxisch-ischemische (HI) ratten te onderzoeken en de cruciale rol van IL-10 bij het verlichten van HI-hersenschade te onderzoeken. In deze studie werden neonatale Sprague-Dawley-ratten onderworpen aan de ligatie van de rechter gemeenschappelijke halsslagader, gevolgd door 2 uur hypoxie.

De expressie van IL-10 in de cortex, hippocampus en longweefsels werd gemeten met immunohistochemie, realtime kwantitatieve polymerasekettingreactie (RT-qPCR) en western blot (WB). Dubbele kleuring met immunofluorescentie werd uitgevoerd om de lokalisatie van IL-10 in neuronen en astrocyten te observeren . Bovendien werden niet-targeting en concentrating on IL-10 siRNA-lentivirusvectoren geïnjecteerd in respectievelijk de ratten van de negatieve controle (NC) en IL-10-groep, en de mRNA-niveaus van B-cellymfoom 2 (Bcl-2) en endoplasmatische reticulum eiwit 29 (ERp29) werden gedetecteerd door RT-qPCR na IL-10 stilte.

De resultaten toonden aan dat de IL-10-expressie aanzienlijk was verhoogd nadat HI en IL-10 waren gecolokaliseerd met neuronen en astrocyten die ernstig waren beschadigd door HI-belediging. Bovendien waren Bcl-2 en ERp29 opmerkelijk verlaagd na IL-10-mRNA-interferentie in vergelijking met de NC-groep. Onze bevindingen onthulden dat IL-10 zijn anti-apoptotische en neuroprotectieve effecten uitoefende door de expressie van Bcl-2 en ERp29 te reguleren, wat aangeeft dat IL-10 een veelbelovend molecuuldoelwit kan zijn voor HIE-behandeling.

GCFC2 Recombinant Protein (Rat)
RP202352	ABM	100 ug	Ask for price

GCFC2 Recombinant Protein (Human)
RP060264	ABM	100 ug	Ask for price

pCMV-SPORT6-GCFC2-G181A
PVT12797	Lifescience Market	2 ug	EUR 469.2

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 336

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 336

Polyclonal Goat anti-GST p-form
GST-ANTI-3	Detroit R&D	50 uL	EUR 336

Gcfc2 ORF Vector (Rat) (pORF)
ORF067452	ABM	1.0 ug DNA	EUR 607.2

GCFC2 ORF Vector (Human) (pORF)
ORF020089	ABM	1.0 ug DNA	Ask for price

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody
20-abx008656	Abbexa	EUR 360.00 EUR 526.80 EUR 226.80	100 ul 200 ul 30 ul

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody
abx330966-100ul	Abbexa	100 ul	EUR 510

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody
20-abx323787	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

GCFC2 Protein Vector (Human) (pPB-C-His)
PV080353	ABM	500 ng	Ask for price

GCFC2 Protein Vector (Human) (pPB-N-His)
PV080354	ABM	500 ng	Ask for price

GCFC2 Protein Vector (Human) (pPM-C-HA)
PV080355	ABM	500 ng	Ask for price

GCFC2 Protein Vector (Human) (pPM-C-His)
PV080356	ABM	500 ng	Ask for price

GCFC2 Protein Vector (Rat) (pPB-C-His)
PV269806	ABM	500 ng	EUR 1399.2

GCFC2 Protein Vector (Rat) (pPB-N-His)
PV269807	ABM	500 ng	EUR 1399.2

GCFC2 Protein Vector (Rat) (pPM-C-HA)
PV269808	ABM	500 ng	EUR 1399.2

GCFC2 Protein Vector (Rat) (pPM-C-His)
PV269809	ABM	500 ng	EUR 1399.2

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV654355	ABM	1.0 ug DNA	EUR 1626

GCFC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV654359	ABM	1.0 ug DNA	EUR 1626

GCFC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV654360	ABM	1.0 ug DNA	EUR 1626

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
LV762095	ABM	1.0 ug DNA	EUR 1104

GCFC2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV762099	ABM	1.0 ug DNA	EUR 1104

GCFC2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV762100	ABM	1.0 ug DNA	EUR 1104

Human Chromosome 2 Open Reading Frame 3 (GCFC2) ELISA Kit
abx386199-96tests	Abbexa	96 tests	EUR 1093.2

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV654356	ABM	1.0 ug DNA	EUR 1626

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV654357	ABM	1.0 ug DNA	EUR 1695.6

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV654358	ABM	1.0 ug DNA	EUR 1695.6

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV762096	ABM	1.0 ug DNA	EUR 1104

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV762097	ABM	1.0 ug DNA	EUR 1173.6

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV762098	ABM	1.0 ug DNA	EUR 1173.6

Positive control tissue section for each individua
Control-Slides-for-each-antibody	Innovex	Set of 25	EUR 355
Description: Positive control tissue section for each individual antibody; Based on availability; INQUIRE

Anti-Anti-SEPT6 antibody antibody
STJ11100949	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody
STJ111369	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT11 Antibody antibody
STJ111530	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT4 Antibody antibody
STJ112276	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-MARCH9 Antibody antibody
STJ112609	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT11 Antibody antibody
STJ113941	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT11 Antibody antibody
STJ114081	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT5 Antibody antibody
STJ114819	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-MARCH8 Antibody antibody
STJ114828	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT7 Antibody antibody
STJ116214	St John's Laboratory	100 µl	EUR 332.4
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody
STJ117206	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody
STJ117759	St John's Laboratory	100 µl	EUR 332.4
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody
STJ118549	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-MARCH6 Antibody antibody
STJ118550	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-MARCH7 Antibody antibody
STJ118752	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT3 Antibody antibody
STJ118990	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT2 Antibody antibody
STJ28365	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT7 Antibody antibody
STJ28963	St John's Laboratory	100 µl	EUR 332.4
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT2 Antibody antibody
STJ25475	St John's Laboratory	100 µl	EUR 332.4

Anti-Anti-SEPT5 Antibody antibody
STJ25477	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody
STJ25479	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT1 antibody antibody
STJ119580	St John's Laboratory	100 µl	EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-DDB1 Antibody
A00333	BosterBio	100uL	EUR 546
Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 6777.36
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 663.31
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 896.16
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3672.72
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
4-AEA465Hu	Cloud-Clone	EUR 6837.60 EUR 3613.20 EUR 896.40	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)
ELK8071	ELK Biotech	1 plate of 96 wells	EUR 518.4
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Anti-ZNF660 antibody
44742-100ul	SAB	100ul	EUR 302.4

Anti-ZNF660 antibody
44742-50ul	SAB	50ul	EUR 224.4

Anti-CCV Antibody
10-2789	Fitzgerald	1 mg	EUR 217.2
Description: Anti-Canine Corona Virus Antibody

Anti-CCV Antibody
10-2790	Fitzgerald	1 mg	EUR 217.2
Description: Anti-Canine Corona Virus Antibody

Anti-CDV antibody
10-2791	Fitzgerald	1 mg	EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CDV antibody
10-2792	Fitzgerald	1 mg	EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CDV antibody
10-2793	Fitzgerald	1 mg	EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CDV antibody
10-2794	Fitzgerald	1 mg	EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CPV Antibody
10-2795	Fitzgerald	500 uig	EUR 165.6
Description: Anti-Canine Parvo Virus Antibody

Anti-CPV Antibody
10-2796	Fitzgerald	500 ug	EUR 165.6
Description: Anti-Canine Parvo Virus Antibody

Anti-CPV Antibody
10-2797	Fitzgerald	500 ug	EUR 165.6
Description: Anti-Canine Parvo Virus Antibody

Anti-C.CRP antibody
10-2798	Fitzgerald	500 ug	EUR 165.6
Description: Anti-Canine CRP antibody

Anti-C.CRP antibody
10-2799	Fitzgerald	500 ug	EUR 165.6
Description: Anti-Canine CRP antibody

Anti-CHW antibody
10-2800	Fitzgerald	1 mg	EUR 198
Description: Anti-Canine Heartworm antibody

Anti-FeLV antibody
10-2801	Fitzgerald	1 mg	EUR 217.2
Description: Anti-Feline Leukemia Virus antibody

Anti-FeLV antibody
10-2802	Fitzgerald	1 mg	EUR 217.2
Description: Anti-Feline Leukemia Virus antibody

Anti-FeLV antibody
10-2803	Fitzgerald	1 mg	EUR 217.2
Description: Anti-Feline Leukemia Virus antibody

Anti-PGI antibody
10-2806	Fitzgerald	1 mg	EUR 502.8
Description: Anti-PGI recombinant antibody

Anti-PGI antibody
10-2807	Fitzgerald	1 mg	EUR 502.8
Description: Anti-PGI recombinant antibody

Anti-PGII antibody
10-2808	Fitzgerald	1 mg	EUR 502.8
Description: Anti-PGII recombinant antibody

Anti-PGII antibody
10-2809	Fitzgerald	1 mg	EUR 502.8
Description: Anti-PGII recombinant antibody

Anti-CHW antibody
10-2810	Fitzgerald	1 mg	EUR 198
Description: Anti-CHW monoclonal antibody

Anti-Giardia antibody
10-2811	Fitzgerald	1 mg	EUR 198
Description: Anti-Giardia monoclonal antibody

Anti-Giardia antibody
10-2812	Fitzgerald	1 mg	EUR 198
Description: Anti-Giardia monoclonal antibody

Anti-TAP1 Antibody
A01123	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal TAP1 Antibody. Validated in IP, IHC, WB and tested in Human, Rat.

Anti-CHD4 Antibody
A01125	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal CHD4 Antibody. Validated in IF, IHC and tested in Human, Mouse.

Anti-NRF1 Antibody
A01129-2	BosterBio	100ug/vial	EUR 400.8

Anti-MEF2C Antibody
A01131-1	BosterBio	100ug/vial	EUR 400.8

Anti-SCN4A Antibody
A01138	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal SCN4A Antibody. Validated in WB and tested in Human.

Anti-MTR Antibody
A01140-2	BosterBio	100ug/vial	EUR 400.8

Anti-IL12B Antibody
A01152-2	BosterBio	100ug/vial	EUR 352.8

Anti-IL12B Antibody
A01152-3	BosterBio	100ug/vial	EUR 400.8

Anti-IL12B Antibody
A01152-4	BosterBio	100ug/vial	EUR 352.8

Anti-IL11 Antibody
A01160-1	BosterBio	100ug/vial	EUR 352.8

Anti-Bak Antibody
A01163	BosterBio	200ug	EUR 626.4
Description: Rabbit Polyclonal Bak Antibody. Validated in IP, IF, IHC, WB and tested in Human.

Anti-PGF Antibody
A01164-1	BosterBio	100ug/vial	EUR 352.8

Anti-PGF Antibody
A01164-2	BosterBio	100ug/vial	EUR 352.8

Anti-CD201 Antibody
A01166	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for CD201 Antibody (PROCR) detection.tested for IHC, WB in Human, Mouse, Rat.

Anti-SERCA2 Antibody
A01176	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for SERCA2 Antibody (ATP2A2) detection.tested for IHC, WB in Human, Mouse, Rat.

Anti-XPA Antibody
A01182	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal XPA Antibody. Validated in IHC, WB and tested in Human.

Anti-XPA Antibody
A01182-1	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for XPA Antibody (XPA) detection.tested for WB in Human, Mouse.

Anti-XPA Antibody
A01182-2	BosterBio	100ug/vial	EUR 352.8

Anti-BNP Antibody
A01186-1	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal BNP Antibody. Validated in IF, IHC, WB and tested in Mouse, Rat.

Anti-CD1c Antibody
A01188-1	BosterBio	100ug/vial	EUR 352.8

Anti-CDC7 Antibody
A01190	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal CDC7 Antibody. Validated in WB and tested in Human.

Anti-VCAM1 Antibody
A01199	BosterBio	100ug/vial	EUR 400.8

Anti-VCAM1 Antibody
A01199-1	BosterBio	100ug/vial	EUR 352.8

Anti-VCAM1 Antibody
A01199-2	BosterBio	100ug/vial	EUR 400.8

Anti-MAP2 Antibody
A01201	BosterBio	100ug/vial	EUR 352.8

Anti-MAP2 Antibody
A01201-1	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal MAP2 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-RING1B Antibody
A01209-1	BosterBio	100ug	EUR 546
Description: Goat Polyclonal RING1B Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-MUC2 Antibody
A01212	BosterBio	100ug/vial	EUR 400.8

Anti-C1INH Antibody
A01221	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for C1INH Antibody (SERPING1) detection.tested for WB in Human.

Anti-WWOX Antibody
A01223-1	BosterBio	100ug/vial	EUR 400.8

Anti-RNF168 Antibody
A01224-1	BosterBio	100ug/vial	EUR 400.8

Anti-HDAC5 Antibody
A01230-5	BosterBio	100ug/vial	EUR 400.8

Anti-Dysferlin Antibody
A01234-1	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Dysferlin Antibody. Validated in WB and tested in Human, Mouse.

Anti-LPP Antibody
A01240-2	BosterBio	100ug/vial	EUR 400.8

Anti-p57 Antibody
A01244	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for p57 Antibody (CDKN1C) detection.tested for IHC, WB in Human, Mouse, Rat.

Anti-MDC1 Antibody
A01252-2	BosterBio	100ug/vial	EUR 400.8

Anti-FHL Antibody
A01258-1	BosterBio	100ug/vial	EUR 400.8

Anti-FHL Antibody
A01258-2	BosterBio	100ug/vial	EUR 352.8

Anti-ACBP Antibody
A01267-1	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for ACBP Antibody (DBI) detection. Tested with WB in Human, Mouse, Rat.

Anti-PDK1 Antibody
A01268	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for PDK1 Antibody (PDK1) detection. Tested with WB in Human, Mouse, Rat.

Anti-CD81 Antibody
A01281-1	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for CD81 Antibody (CD81) detection. Tested with WB in Human, Mouse, Rat.

Anti-CHI3L1 Antibody
A01283-1	BosterBio	100ug/vial	EUR 352.8

Anti-HLF Antibody
A01284-1	BosterBio	100ug/vial	EUR 352.8

Anti-BRD7 Antibody
A01289-1	BosterBio	100ug/vial	EUR 400.8

Anti-Endostatin Antibody
A01302	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Endostatin Antibody. Validated in IHC and tested in Human.

Anti-COL18A1 Antibody
A01302-1	BosterBio	100ug/vial	EUR 352.8

Anti-SATB1 Antibody
A01312-1	BosterBio	100ug/vial	EUR 400.8

Anti-TCF7 Antibody
A01315-2	BosterBio	100ug/vial	EUR 352.8

Anti-WEE1 Antibody
A01319-1	BosterBio	100ug/vial	EUR 352.8

Anti-CD141 Antibody
A01325	BosterBio	100 ug	EUR 476.4
Description: Rabbit Polyclonal CD141 Antibody. Validated in WB and tested in Human.

Anti-NDRG1 Antibody
A01327-1	BosterBio	100ug/vial	EUR 352.8

Anti-FOXP2 Antibody
A01329-1	BosterBio	100ug/vial	EUR 352.8

Anti-CTRO Antibody
A01331	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal CTRO Antibody. Validated in IHC and tested in Human.

Anti-GOLPH3 Antibody
A01333-1	BosterBio	100ug/vial	EUR 400.8

Anti-Aladin Antibody
A01336	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for Aladin Antibody (AAAS) detection. Tested with WB in Human, Rat.

Anti-DBF4 Antibody
A01348-1	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal DBF4 Antibody. Validated in WB and tested in Human, Mouse.

Anti-EIF4G1 Antibody
A01351-2	BosterBio	100ug/vial	EUR 400.8

Anti-DDT Antibody
A01354	BosterBio	100ug/vial	EUR 400.8

Anti-Cdc6 Antibody
A01355	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for Cdc6 Antibody (CDC6) detection.tested for WB in Human, Mouse.

Anti-TRIM5 Antibody
A01359-1	BosterBio	100ug/vial	EUR 352.8

Anti-STAT2 Antibody
A01360	BosterBio	100ug/vial	EUR 352.8

Anti-CDC45L Antibody
A01367-1	BosterBio	100ug/vial	EUR 352.8

Anti-VAPB Antibody
A01372	BosterBio	100ug/vial	EUR 352.8

Anti-IGFBP2 Antibody
A01373-1	BosterBio	100ug/vial	EUR 400.8

Anti-IGFBP2 Antibody
A01373-2	BosterBio	100ug/vial	EUR 400.8

Anti-SphK2 Antibody
A01382	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for SphK2 Antibody (SPHK2) detection.tested for WB in Human, Mouse, Rat.

Anti-SPHK2 Antibody
A01382-1	BosterBio	100ug/vial	EUR 400.8

Anti-ITK Antibody
A01385-2	BosterBio	100ug/vial	EUR 352.8

Anti-NAT1 Antibody
A01386-1	BosterBio	100ug/vial	EUR 352.8

Anti-Tff1 Antibody
A01391-1	BosterBio	100ug/vial	EUR 352.8

Anti-ALDH1A1 Antibody
A01392	BosterBio	100ug/vial	EUR 400.8

Anti-TIP60 Antibody
A01393	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for TIP60 Antibody (KAT5) detection. Tested with WB in Human, Mouse, Rat.

Anti-LIF Antibody
A01400	BosterBio	100ug/vial	EUR 352.8

Anti-RALBP1 Antibody
A01403-1	BosterBio	100ug/vial	EUR 400.8

Anti-Cav3.2 Antibody
A01406	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for Cav3.2 Antibody (CACNA1H) detection. Tested with IHC in Human, Rat, Mouse.

Anti-CARD9 Antibody
A01410-1	BosterBio	100ug/vial	EUR 400.8

Anti-PRMT1 Antibody
A01417-1	BosterBio	100ug	EUR 476.4
Description: Rabbit Polyclonal PRMT1 Antibody. Validated in ELISA, IHC, WB and tested in Human.

Anti-PRMT1 Antibody
A01417-3	BosterBio	100ug/vial	EUR 352.8

Anti-Lyn Antibody
A01424-2	BosterBio	100ug/vial	EUR 400.8

Anti-IL6R Antibody
A01425-1	BosterBio	100ug/vial	EUR 352.8

Anti-PSD Antibody
A01427	BosterBio	100ug/vial	EUR 352.8

Anti-DDB2 Antibody
A01430-2	BosterBio	100ug/vial	EUR 352.8

Anti-FCGR2A Antibody
A01450-1	BosterBio	100ug/vial	EUR 400.8

Anti-FES Antibody
A01453	BosterBio	100ug/vial	EUR 352.8

Anti-TAK1 Antibody
A01458	BosterBio	100ug/vial	EUR 476.4
Description: Rabbit Polyclonal TAK1 Antibody. Validated in IHC and tested in Human, Mouse, Rat.

Anti-GALT Antibody
A01460-1	BosterBio	100ug/vial	EUR 352.8

Anti-BTG2 Antibody
A01461-1	BosterBio	100ug/vial	EUR 400.8

Anti-GSTA1 Antibody
A01462	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for GSTA1 Antibody (GSTA1) detection.tested for IHC in Human.

Anti-ACVL1 Antibody
A01468-1	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal ACVL1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-BNIP3 Antibody
A01469	BosterBio	100ug/vial	EUR 352.8

Anti-TRPC3 Antibody
A01472	BosterBio	100ug/vial	EUR 352.8

Anti-ABCG8 Antibody
A01482-1	BosterBio	100ug/vial	EUR 352.8

Anti-ABCG8 Antibody
A01482-2	BosterBio	100ug/vial	EUR 352.8

Anti-FEN1 Antibody
A01484	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal FEN1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-FEN1 Antibody
A01484-1	BosterBio	100ug/vial	EUR 400.8

Anti-ARHGEF5 Antibody
A01487-1	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal ARHGEF5 Antibody. Validated in WB and tested in Human, Mouse.

Anti-Cdk8 Antibody
A01493	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for Cdk8 Antibody (CDK8) detection.tested for WB in Human, Mouse.

Anti-Cdk8 Antibody
A01493-1	BosterBio	100ug/vial	EUR 352.8

Anti-CRB1 Antibody
A01499	BosterBio	100ug/vial	EUR 400.8

Anti-CRB1 Antibody
A01499-1	BosterBio	100ug/vial	EUR 400.8

Anti-PARN Antibody
A01501-2	BosterBio	100ug/vial	EUR 400.8

Anti-HMBS Antibody
A01506-1	BosterBio	100ug/vial	EUR 352.8

Anti-EPHB2 Antibody
A01507	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal EPHB2 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-CD200 Antibody
A01512	BosterBio	100ul	EUR 476.4
Description: Rabbit Polyclonal Antibody for CD200 Antibody (CD200) detection.tested for IHC, WB in Human.

Gemultiplexte en high-throughput labelvrije detectie van RNA/Spike  Protein /IgG/IgM-biomarkers van SARS-CoV-2-infectie met behulp van nanoplasmonische biosensoren

Om de COVID-19-uitbraak, die wordt veroorzaakt door het ernstige acute respiratoire syndroom coronavirus 2 (SARS-CoV-2), aan te pakken, is er een onvervulde behoefte aan zeer nauwkeurige diagnostische checks in alle stadia van infectie met snelle resultaten en hoge specificiteit. Hier presenteren we een labelvrije, op nanoplasmonische biosensor gebaseerde, multiplex screeningtest voor COVID-19 die 10 verschillende biomarkers kwantitatief kan detecteren (6 virale nucleïnezuurgenen, 2 spike-eiwitsubeenheden en 2 antilichamen) met een detectielimiet in de aM-reeks, allemaal binnen één biosensorplatform.

Onze nieuw ontwikkelde nanoplasmonische biosensoren vertonen een hoge specificiteit, wat van het grootste belang is om valse reacties te voorkomen. Als proof of idea laten we zien dat onze detectiebenadering het potentieel heeft om zowel IgG- als IgM- antilichamen rechtstreeks uit COVID-19-positieve patiëntplasmamonsters te kwantificeren in een enkele instrumentrun, wat de hoge doorvoercapaciteit van onze detectiebenadering aantoont. Het belangrijkste is dat onze take a look at ontvangende operationele kenmerken biedt, gebieden onder de curve van respectievelijk 0,997 en 0,999 voor IgG en IgM. De berekende  p- waarde bepaald met de Mann-Whitney niet-parametrische take a look at is <0,0001 voor beide antilichamen wanneer de take a look at van COVID-19-positieve patiënten ( n = 80) wordt vergeleken met die van gezonde personen ( n  = 72).

Bovendien geeft de screeningstest een berekende sensitiviteit (true constructive fee) van 100% (80/80), een specificiteit (true negatieve fee) >96% (77/80) , een positief voorspellende waarde van 98% bij een prevalentie van 5% en een negatief voorspellende waarde van 100% bij een prevalentie van 5%. Wij zijn van mening dat onze zeer gevoelige, multiplex, high-throughput testbenadering potentiële toepassingen heeft in de COVID-19-diagnostiek , met title bij het bepalen van virusprogressie en infectie-ernst voor clinici voor een geschikte behandeling, en ook een zeer effectieve diagnostische take a look at zal blijken te zijn wanneer toegepast op ziekten buiten de COVID-19-pandemie.

Nauwkeurigheid van alternatieve niet-polariseerbare krachtvelden voor de bepaling van  eiwit- ligandbindingsaffiniteiten gedomineerd door kation-π-interacties

Het aanpassen van paarspecifieke Lennard-Jones-parameters by way of de niet-gebonden FIX (NBFIX)-functie van het CHARMM36-krachtveld is kosteneffectief gebleken voor het verbeteren van de beschrijving van kation-π-interacties in biologische objecten door middel van paarsgewijs additieve potentiële energiefuncties. Hier worden twee units nieuw geoptimaliseerde CHARMM36-krachtveldparameters, waaronder NBFIX-correcties, bedacht CHARMM36m-NBF en CHARMM36-WYF, en de originele krachtvelden, namelijk CHARMM36m en Amber ff14SB, gebruikt om de standaard bindingsvrije energieën van zeven eiwit- ligandcomplexen die kation-π-interacties bevatten.

Vergeleken met nauwkeurige experimentele metingen, geven onze resultaten aan dat de ongecorrigeerde, originele krachtvelden de bindingsvrije energieën vital onderschatten, met een gemiddelde fout van 5,Three kcal/mol, terwijl de gemiddelde fouten van CHARMM36m-NBF en CHARMM36-WYF 0,eight en 2,1 bedragen. respectievelijk kcal/mol. De huidige studie toont overtuigend aan dat het gebruik van gewijzigde parameters samen met NBFIX-correcties de nauwkeurigheid van de standaard bindingsvrije energie van eiwit-ligandcomplexen die worden gedomineerd door kation-π-interacties, met title met CHARMM36m-NBF, dramatisch verhoogt.

Gebruikmakend van door tijd opgeloste, door  proteïne geïnduceerde fluorescentieverbetering om stabiele lokale conformaties te identificeren, één α-synucleïne-monomeer per keer

Het gebruik van spectroscopische linialen om meerdere conformaties van afzonderlijke biomoleculen en hun dynamiek te volgen, heeft een revolutie teweeggebracht in het begrip van structurele dynamiek en zijn bijdragen aan de biologie. Terwijl de op FRET gebaseerde liniaal rapporteert over afstanden tussen kleurstoffen in het bereik van 3-10 nm, rapporteren andere spectroscopische technieken, zoals eiwit-geïnduceerde fluorescentieverbetering (PIFE), over de nabijheid tussen een kleurstof en een eiwitoppervlak in de kortere 0 -Three nm bereik. Ongeacht de gekozen methode, het gebruik ervan bij het één voor één meten van vrij diffunderende biomoleculen haalt histogrammen op van de experimentele parameter die afzonderlijke centraal verdeelde subpopulaties van biomoleculen oplevert, waarbij elke subpopulatie vertegenwoordigtofwel een enkele conformatie die binnen milliseconden onveranderd bleef, of meerdere conformaties die veel sneller dan milliseconden in elkaar overgaan, en dus een uitgemiddelde subpopulatie.

In FRET met één molecuul, waar de gerapporteerde parameter in histogrammen de FRET-efficiëntie tussen kleurstoffen is, werd eerder gerapporteerd dat een intrinsiek ongeordend eiwit, zoals het α-synucleïne-monomeer in buffer, een enkele gemiddelde subpopulatie van meerdere conformaties die snel in elkaar overgaan. Hoewel deze eerdere bevindingen afhankelijk zijn van het bereik van 3-10 nm van de FRET-gebaseerde liniaal, hebben we geprobeerd dit eiwit op de proef te stellen met behulp van PIFE met één molecuul, waarbij we de fluorescentielevensduur van plaatsspecifieke sCy3-gelabelde α-Synuclein volgen. eiwitten één voor één. Interessant is dat met behulp van deze spectroscopische naderingssensor met een kortere afstand, sCy3-gelabeld -Synucleïne verschillende subpopulaties met een duidelijk verschillende gemiddelde levensduur vertoont die in 10-100 ms in elkaar overgaan.Deze resultaten laten zien dat, hoewel α-synucleïne wereldwijd ontregeld kan zijn, het toch stabiele lokale structuren bereikt. Samenvattend, in dit werk benadrukken we het voordeel van het gebruik van verschillende spectroscopische nabijheidssensoren die lokale of globale structurele veranderingen één biomolecuul tegelijk volgen.