unitech-europe

Nauwkeurigheid van alternatieve niet-polariseerbare krachtvelden

SPC1 regelt de signaal-peptidase bemiddelde verwerken van membraan  proteïnen

Signaalpeptidase (SPase) splitst de signaalsequenties (SS’s ) van secretoire voorlopers. Het bevat een evolutionair geconserveerde membraaneiwitsubeenheid, Spcl, die niet nodig is voor de katalytische activiteit van SPase, en de rol ervan blijft onbekend. In de huidige studie hebben we de functie van gist Spc1 onderzocht. Eerst hebben we een in vivo SPase-splitsingstest opgezet met behulp van secretoire eiwit-CPY- varianten met SS’s gemodificeerd in de n- en h-regio’s.

Bij het vergelijken van de SS-splitsingsefficiëntie van deze varianten in cellen met of zonder Spcl, ontdekten we dat signaalverankerde sequenties vatbaarder werden voor splitsing door SPase zonder Spcl. Verder was SPase-gemedieerde verwerking van modelmembraaneiwitten verbeterd in de afwezigheid van maar verminderd bij overexpressie van Spcl. Spcl werd geco-immunoprecipiteerd met eiwitten die niet-gesplitste signaalverankerde of transmembraan (TM) segmenten droegen.

Samengevat suggereren deze resultaten dat Spc1 TM-segmenten beschermt tegen SPase-actie, waardoor de substraatselectie voor SPase wordt verscherpt en fungeert als een negatieve regulator voor de SPase-gemedieerde verwerking van membraaneiwitten .

Interleukine-10 speelt een belangrijke rol in pasgeboren ratten met Hypoxic-ischemie geassocieerd met B-cel lymfoom 2 en endoplasmatisch reticulum  Protein  29

 

Interleukine 10 (IL-10) is een synthetische remmer van humane cytokines met immunomodulerende en ontstekingsremmende effecten. Deze studie was bedoeld om de expressievariatie van IL-10 op meerdere plaatsen, waaronder cortex, hippocampus en longweefsels van neonatale hypoxisch-ischemische (HI) ratten te onderzoeken en de cruciale rol van IL-10 bij het verlichten van HI-hersenschade te onderzoeken. In deze studie werden neonatale Sprague-Dawley-ratten onderworpen aan de ligatie van de rechter gemeenschappelijke halsslagader, gevolgd door 2 uur hypoxie.

De expressie van IL-10 in de cortex, hippocampus en longweefsels werd gemeten met immunohistochemie, realtime kwantitatieve polymerasekettingreactie (RT-qPCR) en western blot (WB). Dubbele kleuring met immunofluorescentie werd uitgevoerd om de lokalisatie van IL-10 in neuronen en astrocyten te observeren . Bovendien werden niet-targeting en concentrating on IL-10 siRNA-lentivirusvectoren geïnjecteerd in respectievelijk de ratten van de negatieve controle (NC) en IL-10-groep, en de mRNA-niveaus van B-cellymfoom 2 (Bcl-2) en endoplasmatische reticulum eiwit 29 (ERp29) werden gedetecteerd door RT-qPCR na IL-10 stilte.

De resultaten toonden aan dat de IL-10-expressie aanzienlijk was verhoogd nadat HI en IL-10 waren gecolokaliseerd met neuronen en astrocyten die ernstig waren beschadigd door HI-belediging. Bovendien waren Bcl-2 en ERp29 opmerkelijk verlaagd na IL-10-mRNA-interferentie in vergelijking met de NC-groep. Onze bevindingen onthulden dat IL-10 zijn anti-apoptotische en neuroprotectieve effecten uitoefende door de expressie van Bcl-2 en ERp29 te reguleren, wat aangeeft dat IL-10 een veelbelovend molecuuldoelwit kan zijn voor HIE-behandeling.

unitech-europe
unitech-europe

GCFC2 Recombinant Protein (Rat)

RP202352 100 ug Ask for price

GCFC2 Recombinant Protein (Human)

RP060264 100 ug Ask for price

pCMV-SPORT6-GCFC2-G181A

PVT12797 2 ug
EUR 469.2

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

Gcfc2 ORF Vector (Rat) (pORF)

ORF067452 1.0 ug DNA
EUR 607.2

GCFC2 ORF Vector (Human) (pORF)

ORF020089 1.0 ug DNA Ask for price

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody

20-abx008656
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody

abx330966-100ul 100 ul
EUR 510

Chromosome 2 Open Reading Frame 3 (GCFC2) Antibody

20-abx323787
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

GCFC2 Protein Vector (Human) (pPB-C-His)

PV080353 500 ng Ask for price

GCFC2 Protein Vector (Human) (pPB-N-His)

PV080354 500 ng Ask for price

GCFC2 Protein Vector (Human) (pPM-C-HA)

PV080355 500 ng Ask for price

GCFC2 Protein Vector (Human) (pPM-C-His)

PV080356 500 ng Ask for price

GCFC2 Protein Vector (Rat) (pPB-C-His)

PV269806 500 ng
EUR 1399.2

GCFC2 Protein Vector (Rat) (pPB-N-His)

PV269807 500 ng
EUR 1399.2

GCFC2 Protein Vector (Rat) (pPM-C-HA)

PV269808 500 ng
EUR 1399.2

GCFC2 Protein Vector (Rat) (pPM-C-His)

PV269809 500 ng
EUR 1399.2

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV654355 1.0 ug DNA
EUR 1626

GCFC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV654359 1.0 ug DNA
EUR 1626

GCFC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV654360 1.0 ug DNA
EUR 1626

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

LV762095 1.0 ug DNA
EUR 1104

GCFC2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV762099 1.0 ug DNA
EUR 1104

GCFC2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)

LV762100 1.0 ug DNA
EUR 1104

Human Chromosome 2 Open Reading Frame 3 (GCFC2) ELISA Kit

abx386199-96tests 96 tests
EUR 1093.2

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV654356 1.0 ug DNA
EUR 1626

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV654357 1.0 ug DNA
EUR 1695.6

GCFC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV654358 1.0 ug DNA
EUR 1695.6

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV762096 1.0 ug DNA
EUR 1104

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV762097 1.0 ug DNA
EUR 1173.6

GCFC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV762098 1.0 ug DNA
EUR 1173.6

Anti-Anti-SEPT6 antibody antibody

STJ11100949 100 µl
EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody

STJ111369 100 µl
EUR 332.4
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT11 Antibody antibody

STJ111530 100 µl
EUR 332.4

Anti-Anti-SEPT4 Antibody antibody

STJ112276 100 µl
EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-MARCH9 Antibody antibody

STJ112609 100 µl
EUR 332.4

Anti-Anti-SEPT11 Antibody antibody

STJ113941 100 µl
EUR 332.4

Anti-Anti-SEPT11 Antibody antibody

STJ114081 100 µl
EUR 332.4

Anti-Anti-SEPT5 Antibody antibody

STJ114819 100 µl
EUR 332.4
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-MARCH8 Antibody antibody

STJ114828 100 µl
EUR 332.4

Anti-Anti-SEPT7 Antibody antibody

STJ116214 100 µl
EUR 332.4
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody

STJ117206 100 µl
EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody

STJ117759 100 µl
EUR 332.4
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody

STJ118549 100 µl
EUR 332.4

Anti-Anti-MARCH6 Antibody antibody

STJ118550 100 µl
EUR 332.4

Anti-Anti-MARCH7 Antibody antibody

STJ118752 100 µl
EUR 332.4

Anti-Anti-SEPT3 Antibody antibody

STJ118990 100 µl
EUR 332.4

Anti-Anti-SEPT2 Antibody antibody

STJ28365 100 µl
EUR 332.4

Anti-Anti-SEPT7 Antibody antibody

STJ28963 100 µl
EUR 332.4
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT2 Antibody antibody

STJ25475 100 µl
EUR 332.4

Anti-Anti-SEPT5 Antibody antibody

STJ25477 100 µl
EUR 332.4
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody

STJ25479 100 µl
EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT1 antibody antibody

STJ119580 100 µl
EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-DDB1 Antibody

A00333 100uL
EUR 546
Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-10x96wellstestplate 10x96-wells test plate
EUR 6777.36
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-1x48wellstestplate 1x48-wells test plate
EUR 663.31
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-1x96wellstestplate 1x96-wells test plate
EUR 896.16
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-5x96wellstestplate 5x96-wells test plate
EUR 3672.72
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

4-AEA465Hu
  • EUR 6837.60
  • EUR 3613.20
  • EUR 896.40
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)

ELK8071 1 plate of 96 wells
EUR 518.4
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Anti-ZNF660 antibody

44742-100ul 100ul
EUR 302.4

Anti-ZNF660 antibody

44742-50ul 50ul
EUR 224.4

Anti-CCV Antibody

10-2789 1 mg
EUR 217.2
Description: Anti-Canine Corona Virus Antibody

Anti-CCV Antibody

10-2790 1 mg
EUR 217.2
Description: Anti-Canine Corona Virus Antibody

Anti-CDV antibody

10-2791 1 mg
EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CDV antibody

10-2792 1 mg
EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CDV antibody

10-2793 1 mg
EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CDV antibody

10-2794 1 mg
EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CPV Antibody

10-2795 500 uig
EUR 165.6
Description: Anti-Canine Parvo Virus Antibody

Anti-CPV Antibody

10-2796 500 ug
EUR 165.6
Description: Anti-Canine Parvo Virus Antibody

Anti-CPV Antibody

10-2797 500 ug
EUR 165.6
Description: Anti-Canine Parvo Virus Antibody

Anti-C.CRP antibody

10-2798 500 ug
EUR 165.6
Description: Anti-Canine CRP antibody

Anti-C.CRP antibody

10-2799 500 ug
EUR 165.6
Description: Anti-Canine CRP antibody

Anti-CHW antibody

10-2800 1 mg
EUR 198
Description: Anti-Canine Heartworm antibody

Anti-FeLV antibody

10-2801 1 mg
EUR 217.2
Description: Anti-Feline Leukemia Virus antibody

Anti-FeLV antibody

10-2802 1 mg
EUR 217.2
Description: Anti-Feline Leukemia Virus antibody

Anti-FeLV antibody

10-2803 1 mg
EUR 217.2
Description: Anti-Feline Leukemia Virus antibody

Anti-PGI antibody

10-2806 1 mg
EUR 502.8
Description: Anti-PGI recombinant antibody

Anti-PGI antibody

10-2807 1 mg
EUR 502.8
Description: Anti-PGI recombinant antibody

Anti-PGII antibody

10-2808 1 mg
EUR 502.8
Description: Anti-PGII recombinant antibody

Anti-PGII antibody

10-2809 1 mg
EUR 502.8
Description: Anti-PGII recombinant antibody

Anti-CHW antibody

10-2810 1 mg
EUR 198
Description: Anti-CHW monoclonal antibody

Anti-Giardia antibody

10-2811 1 mg
EUR 198
Description: Anti-Giardia monoclonal antibody

Anti-Giardia antibody

10-2812 1 mg
EUR 198
Description: Anti-Giardia monoclonal antibody

Anti-TAP1 Antibody

A01123 100ul
EUR 476.4
Description: Rabbit Polyclonal TAP1 Antibody. Validated in IP, IHC, WB and tested in Human, Rat.

Anti-CHD4 Antibody

A01125 100ul
EUR 476.4
Description: Rabbit Polyclonal CHD4 Antibody. Validated in IF, IHC and tested in Human, Mouse.

Anti-NRF1 Antibody

A01129-2 100ug/vial
EUR 400.8

Anti-MEF2C Antibody

A01131-1 100ug/vial
EUR 400.8

Anti-SCN4A Antibody

A01138 100ul
EUR 476.4
Description: Rabbit Polyclonal SCN4A Antibody. Validated in WB and tested in Human.

Anti-MTR Antibody

A01140-2 100ug/vial
EUR 400.8

Anti-IL12B Antibody

A01152-2 100ug/vial
EUR 352.8

Anti-IL12B Antibody

A01152-3 100ug/vial
EUR 400.8

Anti-IL12B Antibody

A01152-4 100ug/vial
EUR 352.8

Anti-IL11 Antibody

A01160-1 100ug/vial
EUR 352.8

Anti-Bak Antibody

A01163 200ug
EUR 626.4
Description: Rabbit Polyclonal Bak Antibody. Validated in IP, IF, IHC, WB and tested in Human.

Anti-PGF Antibody

A01164-1 100ug/vial
EUR 352.8

Anti-PGF Antibody

A01164-2 100ug/vial
EUR 352.8

Anti-CD201 Antibody

A01166 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for CD201 Antibody (PROCR) detection.tested for IHC, WB in Human, Mouse, Rat.

Anti-SERCA2 Antibody

A01176 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for SERCA2 Antibody (ATP2A2) detection.tested for IHC, WB in Human, Mouse, Rat.

Anti-XPA Antibody

A01182 100ul
EUR 476.4
Description: Rabbit Polyclonal XPA Antibody. Validated in IHC, WB and tested in Human.

Anti-XPA Antibody

A01182-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for XPA Antibody (XPA) detection.tested for WB in Human, Mouse.

Anti-XPA Antibody

A01182-2 100ug/vial
EUR 352.8

Anti-BNP Antibody

A01186-1 100ul
EUR 476.4
Description: Rabbit Polyclonal BNP Antibody. Validated in IF, IHC, WB and tested in Mouse, Rat.

Anti-CD1c Antibody

A01188-1 100ug/vial
EUR 352.8

Anti-CDC7 Antibody

A01190 100ul
EUR 476.4
Description: Rabbit Polyclonal CDC7 Antibody. Validated in WB and tested in Human.

Anti-VCAM1 Antibody

A01199 100ug/vial
EUR 400.8

Anti-VCAM1 Antibody

A01199-1 100ug/vial
EUR 352.8

Anti-VCAM1 Antibody

A01199-2 100ug/vial
EUR 400.8

Anti-MAP2 Antibody

A01201 100ug/vial
EUR 352.8

Anti-MAP2 Antibody

A01201-1 100ul
EUR 476.4
Description: Rabbit Polyclonal MAP2 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-RING1B Antibody

A01209-1 100ug
EUR 546
Description: Goat Polyclonal RING1B Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-MUC2 Antibody

A01212 100ug/vial
EUR 400.8

Anti-C1INH Antibody

A01221 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for C1INH Antibody (SERPING1) detection.tested for WB in Human.

Anti-WWOX Antibody

A01223-1 100ug/vial
EUR 400.8

Anti-RNF168 Antibody

A01224-1 100ug/vial
EUR 400.8

Anti-HDAC5 Antibody

A01230-5 100ug/vial
EUR 400.8

Anti-Dysferlin Antibody

A01234-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Dysferlin Antibody. Validated in WB and tested in Human, Mouse.

Anti-LPP Antibody

A01240-2 100ug/vial
EUR 400.8

Anti-p57 Antibody

A01244 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for p57 Antibody (CDKN1C) detection.tested for IHC, WB in Human, Mouse, Rat.

Anti-MDC1 Antibody

A01252-2 100ug/vial
EUR 400.8

Anti-FHL Antibody

A01258-1 100ug/vial
EUR 400.8

Anti-FHL Antibody

A01258-2 100ug/vial
EUR 352.8

Anti-ACBP Antibody

A01267-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for ACBP Antibody (DBI) detection. Tested with WB in Human, Mouse, Rat.

Anti-PDK1 Antibody

A01268 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for PDK1 Antibody (PDK1) detection. Tested with WB in Human, Mouse, Rat.

Anti-CD81 Antibody

A01281-1 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for CD81 Antibody (CD81) detection. Tested with WB in Human, Mouse, Rat.

Anti-CHI3L1 Antibody

A01283-1 100ug/vial
EUR 352.8

Anti-HLF Antibody

A01284-1 100ug/vial
EUR 352.8

Anti-BRD7 Antibody

A01289-1 100ug/vial
EUR 400.8

Anti-Endostatin Antibody

A01302 100ul
EUR 476.4
Description: Rabbit Polyclonal Endostatin Antibody. Validated in IHC and tested in Human.

Anti-COL18A1 Antibody

A01302-1 100ug/vial
EUR 352.8

Anti-SATB1 Antibody

A01312-1 100ug/vial
EUR 400.8

Anti-TCF7 Antibody

A01315-2 100ug/vial
EUR 352.8

Anti-WEE1 Antibody

A01319-1 100ug/vial
EUR 352.8

Anti-CD141 Antibody

A01325 100 ug
EUR 476.4
Description: Rabbit Polyclonal CD141 Antibody. Validated in WB and tested in Human.

Anti-NDRG1 Antibody

A01327-1 100ug/vial
EUR 352.8

Anti-FOXP2 Antibody

A01329-1 100ug/vial
EUR 352.8

Anti-CTRO Antibody

A01331 100ul
EUR 476.4
Description: Rabbit Polyclonal CTRO Antibody. Validated in IHC and tested in Human.

Anti-GOLPH3 Antibody

A01333-1 100ug/vial
EUR 400.8

Anti-Aladin Antibody

A01336 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Aladin Antibody (AAAS) detection. Tested with WB in Human, Rat.

Anti-DBF4 Antibody

A01348-1 100ul
EUR 476.4
Description: Rabbit Polyclonal DBF4 Antibody. Validated in WB and tested in Human, Mouse.

Anti-EIF4G1 Antibody

A01351-2 100ug/vial
EUR 400.8

Anti-DDT Antibody

A01354 100ug/vial
EUR 400.8

Anti-Cdc6 Antibody

A01355 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Cdc6 Antibody (CDC6) detection.tested for WB in Human, Mouse.

Anti-TRIM5 Antibody

A01359-1 100ug/vial
EUR 352.8

Anti-STAT2 Antibody

A01360 100ug/vial
EUR 352.8

Anti-CDC45L Antibody

A01367-1 100ug/vial
EUR 352.8

Anti-VAPB Antibody

A01372 100ug/vial
EUR 352.8

Anti-IGFBP2 Antibody

A01373-1 100ug/vial
EUR 400.8

Anti-IGFBP2 Antibody

A01373-2 100ug/vial
EUR 400.8

Anti-SphK2 Antibody

A01382 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for SphK2 Antibody (SPHK2) detection.tested for WB in Human, Mouse, Rat.

Anti-SPHK2 Antibody

A01382-1 100ug/vial
EUR 400.8

Anti-ITK Antibody

A01385-2 100ug/vial
EUR 352.8

Anti-NAT1 Antibody

A01386-1 100ug/vial
EUR 352.8

Anti-Tff1 Antibody

A01391-1 100ug/vial
EUR 352.8

Anti-ALDH1A1 Antibody

A01392 100ug/vial
EUR 400.8

Anti-TIP60 Antibody

A01393 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for TIP60 Antibody (KAT5) detection. Tested with WB in Human, Mouse, Rat.

Anti-LIF Antibody

A01400 100ug/vial
EUR 352.8

Anti-RALBP1 Antibody

A01403-1 100ug/vial
EUR 400.8

Anti-Cav3.2 Antibody

A01406 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Cav3.2 Antibody (CACNA1H) detection. Tested with IHC in Human, Rat, Mouse.

Anti-CARD9 Antibody

A01410-1 100ug/vial
EUR 400.8

Anti-PRMT1 Antibody

A01417-1 100ug
EUR 476.4
Description: Rabbit Polyclonal PRMT1 Antibody. Validated in ELISA, IHC, WB and tested in Human.

Anti-PRMT1 Antibody

A01417-3 100ug/vial
EUR 352.8

Anti-Lyn Antibody

A01424-2 100ug/vial
EUR 400.8

Anti-IL6R Antibody

A01425-1 100ug/vial
EUR 352.8

Anti-PSD Antibody

A01427 100ug/vial
EUR 352.8

Anti-DDB2 Antibody

A01430-2 100ug/vial
EUR 352.8

Anti-FCGR2A Antibody

A01450-1 100ug/vial
EUR 400.8

Anti-FES Antibody

A01453 100ug/vial
EUR 352.8

Anti-TAK1 Antibody

A01458 100ug/vial
EUR 476.4
Description: Rabbit Polyclonal TAK1 Antibody. Validated in IHC and tested in Human, Mouse, Rat.

Anti-GALT Antibody

A01460-1 100ug/vial
EUR 352.8

Anti-BTG2 Antibody

A01461-1 100ug/vial
EUR 400.8

Anti-GSTA1 Antibody

A01462 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for GSTA1 Antibody (GSTA1) detection.tested for IHC in Human.

Anti-ACVL1 Antibody

A01468-1 100ul
EUR 476.4
Description: Rabbit Polyclonal ACVL1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-BNIP3 Antibody

A01469 100ug/vial
EUR 352.8

Anti-TRPC3 Antibody

A01472 100ug/vial
EUR 352.8

Anti-ABCG8 Antibody

A01482-1 100ug/vial
EUR 352.8

Anti-ABCG8 Antibody

A01482-2 100ug/vial
EUR 352.8

Anti-FEN1 Antibody

A01484 100ul
EUR 476.4
Description: Rabbit Polyclonal FEN1 Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-FEN1 Antibody

A01484-1 100ug/vial
EUR 400.8

Anti-ARHGEF5 Antibody

A01487-1 100ul
EUR 476.4
Description: Rabbit Polyclonal ARHGEF5 Antibody. Validated in WB and tested in Human, Mouse.

Anti-Cdk8 Antibody

A01493 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for Cdk8 Antibody (CDK8) detection.tested for WB in Human, Mouse.

Anti-Cdk8 Antibody

A01493-1 100ug/vial
EUR 352.8

Anti-CRB1 Antibody

A01499 100ug/vial
EUR 400.8

Anti-CRB1 Antibody

A01499-1 100ug/vial
EUR 400.8

Anti-PARN Antibody

A01501-2 100ug/vial
EUR 400.8

Anti-HMBS Antibody

A01506-1 100ug/vial
EUR 352.8

Anti-EPHB2 Antibody

A01507 100ul
EUR 476.4
Description: Rabbit Polyclonal EPHB2 Antibody. Validated in WB and tested in Human, Mouse, Rat.

Anti-CD200 Antibody

A01512 100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for CD200 Antibody (CD200) detection.tested for IHC, WB in Human.

Anti-CD31 Antibody

A01513 100uL
EUR 531.6
Description: Rabbit Polyclonal CD31 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Gemultiplexte en high-throughput labelvrije detectie van RNA/Spike  Protein /IgG/IgM-biomarkers van SARS-CoV-2-infectie met behulp van nanoplasmonische biosensoren

Om de COVID-19-uitbraak, die wordt veroorzaakt door het ernstige acute respiratoire syndroom coronavirus 2 (SARS-CoV-2), aan te pakken, is er een onvervulde behoefte aan zeer nauwkeurige diagnostische checks in alle stadia van infectie met snelle resultaten en hoge specificiteit. Hier presenteren we een labelvrije, op nanoplasmonische biosensor gebaseerde, multiplex screeningtest voor COVID-19 die 10 verschillende biomarkers kwantitatief kan detecteren (6 virale nucleïnezuurgenen, 2 spike-eiwitsubeenheden en 2 antilichamen) met een detectielimiet in de aM-reeks, allemaal binnen één biosensorplatform.

Onze nieuw ontwikkelde nanoplasmonische biosensoren vertonen een hoge specificiteit, wat van het grootste belang is om valse reacties te voorkomen. Als proof of idea laten we zien dat onze detectiebenadering het potentieel heeft om zowel IgG- als IgM- antilichamen rechtstreeks uit COVID-19-positieve patiëntplasmamonsters te kwantificeren in een enkele instrumentrun, wat de hoge doorvoercapaciteit van onze detectiebenadering aantoont. Het belangrijkste is dat onze take a look at ontvangende operationele kenmerken biedt, gebieden onder de curve van respectievelijk 0,997 en 0,999 voor IgG en IgM. De berekende  p- waarde bepaald met de Mann-Whitney niet-parametrische take a look at is <0,0001 voor beide antilichamen wanneer de take a look at van COVID-19-positieve patiënten ( n = 80) wordt vergeleken met die van gezonde personen ( n  = 72).

Bovendien geeft de screeningstest een berekende sensitiviteit (true constructive fee) van 100% (80/80), een specificiteit (true negatieve fee) >96% (77/80) , een positief voorspellende waarde van 98% bij een prevalentie van 5% en een negatief voorspellende waarde van 100% bij een prevalentie van 5%. Wij zijn van mening dat onze zeer gevoelige, multiplex, high-throughput testbenadering potentiële toepassingen heeft in de COVID-19-diagnostiek , met title bij het bepalen van virusprogressie en infectie-ernst voor clinici voor een geschikte behandeling, en ook een zeer effectieve diagnostische take a look at zal blijken te zijn wanneer toegepast op ziekten buiten de COVID-19-pandemie.

Nauwkeurigheid van alternatieve niet-polariseerbare krachtvelden voor de bepaling van  eiwit- ligandbindingsaffiniteiten gedomineerd door kation-π-interacties

Het aanpassen van paarspecifieke Lennard-Jones-parameters by way of de niet-gebonden FIX (NBFIX)-functie van het CHARMM36-krachtveld is kosteneffectief gebleken voor het verbeteren van de beschrijving van kation-π-interacties in biologische objecten door middel van paarsgewijs additieve potentiële energiefuncties. Hier worden twee units nieuw geoptimaliseerde CHARMM36-krachtveldparameters, waaronder NBFIX-correcties, bedacht CHARMM36m-NBF en CHARMM36-WYF, en de originele krachtvelden, namelijk CHARMM36m en Amber ff14SB, gebruikt om de standaard bindingsvrije energieën van zeven eiwit- ligandcomplexen die kation-π-interacties bevatten.

Vergeleken met nauwkeurige experimentele metingen, geven onze resultaten aan dat de ongecorrigeerde, originele krachtvelden de bindingsvrije energieën vital onderschatten, met een gemiddelde fout van 5,Three kcal/mol, terwijl de gemiddelde fouten van CHARMM36m-NBF en CHARMM36-WYF 0,eight en 2,1 bedragen. respectievelijk kcal/mol. De huidige studie toont overtuigend aan dat het gebruik van gewijzigde parameters samen met NBFIX-correcties de nauwkeurigheid van de standaard bindingsvrije energie van eiwit-ligandcomplexen die worden gedomineerd door kation-π-interacties, met title met CHARMM36m-NBF, dramatisch verhoogt.

Gebruikmakend van door tijd opgeloste, door  proteïne geïnduceerde fluorescentieverbetering om stabiele lokale conformaties te identificeren, één α-synucleïne-monomeer per keer

Het gebruik van spectroscopische linialen om meerdere conformaties van afzonderlijke biomoleculen en hun dynamiek te volgen, heeft een revolutie teweeggebracht in het begrip van structurele dynamiek en zijn bijdragen aan de biologie. Terwijl de op FRET gebaseerde liniaal rapporteert over afstanden tussen kleurstoffen in het bereik van 3-10 nm, rapporteren andere spectroscopische technieken, zoals eiwit-geïnduceerde fluorescentieverbetering (PIFE), over de nabijheid tussen een kleurstof en een eiwitoppervlak in de kortere 0 -Three nm bereik. Ongeacht de gekozen methode, het gebruik ervan bij het één voor één meten van vrij diffunderende biomoleculen haalt histogrammen op van de experimentele parameter die afzonderlijke centraal verdeelde subpopulaties van biomoleculen oplevert, waarbij elke subpopulatie vertegenwoordigtofwel een enkele conformatie die binnen milliseconden onveranderd bleef, of meerdere conformaties die veel sneller dan milliseconden in elkaar overgaan, en dus een uitgemiddelde subpopulatie.

In FRET met één molecuul, waar de gerapporteerde parameter in histogrammen de FRET-efficiëntie tussen kleurstoffen is, werd eerder gerapporteerd dat een intrinsiek ongeordend eiwit, zoals het α-synucleïne-monomeer in buffer, een enkele gemiddelde subpopulatie van meerdere conformaties die snel in elkaar overgaan. Hoewel deze eerdere bevindingen afhankelijk zijn van het bereik van 3-10 nm van de FRET-gebaseerde liniaal, hebben we geprobeerd dit eiwit op de proef te stellen met behulp van PIFE met één molecuul, waarbij we de fluorescentielevensduur van plaatsspecifieke sCy3-gelabelde α-Synuclein volgen. eiwitten één voor één. Interessant is dat met behulp van deze spectroscopische naderingssensor met een kortere afstand, sCy3-gelabeld -Synucleïne verschillende subpopulaties met een duidelijk verschillende gemiddelde levensduur vertoont die in 10-100 ms in elkaar overgaan.Deze resultaten laten zien dat, hoewel α-synucleïne wereldwijd ontregeld kan zijn, het toch stabiele lokale structuren bereikt. Samenvattend, in dit werk benadrukken we het voordeel van het gebruik van verschillende spectroscopische nabijheidssensoren die lokale of globale structurele veranderingen één biomolecuul tegelijk volgen.

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