Combinatie van snelle microfluïdische menging en driekleurige enkel-molecuul FRET voor het onderzoeken van de kinetiek van eiwitconformationele veranderingen
Förster-resonantie-energieoverdracht met één molecuul (FRET) is zeer geschikt voor het bestuderen van de kinetiek van eiwitconformatieveranderingen, vanwege de hoge gevoeligheid en het vermogen om individuele subpopulaties in heterogene systemen op te lossen . De meest gebruikelijke benadering waarbij twee fluoroforen worden gebruikt, kan echter slechts één afstand tegelijk bewaken, en het gebruik van drie fluoroforen voor het gelijktijdig bewaken van meerdere afstanden is grotendeels beperkt tot evenwichtsfluctuaties. Hier laten we zien dat driekleurige FRET met één molecuul kan worden gecombineerd met snelle microfluïdische menging om conformationele veranderingen in een eiwit van milliseconden tot minuten te onderzoeken. In combinatie met handmatig mengen hebben we de kinetiek uitgebreid tot 1 uur, wat overeenkomt met een totaal bereik van 5 ordes van grootte in de tijd.
We bestudeerden de omzetting van monomeer naar protomeer van het porievormende toxine cytolysine A (ClyA), een van de grootste bekende conformatieovergangen van eiwitten. Plaatsspecifieke labeling van ClyA met drie fluoroforen stelde ons in staat om de kinetiek van drie intramoleculaire afstanden tegelijkertijd te volgen en onthulde een voorheen onopgemerkt tussenproduct. De combinatie van driekleurige FRET met één molecuul met snelle microfluïdische menging biedt dus een benadering voor het onderzoeken van de mechanismen van complexe biomoleculaire processen met een hoge tijdsresolutie.
Kankerimmunotherapie door NC410, een LAIR-2 Fc- eiwit dat menselijke LAIR-collageeninteractie blokkeert
Collagenen zijn een primaire part van de extracellulaire matrix en zijn functionele liganden voor de remmende immuunreceptor leukocyt-geassocieerde immunoglobuline-achtige receptor (LAIR)-1. LAIR-2 is een uitgescheiden eiwit dat kan fungeren als een lokreceptor door collageen te binden met een hogere affiniteit dan LAIR-1. We stellen voor dat collageen immuunontwijking bevordert door interactie met LAIR-1 dat tot expressie wordt gebracht op immuuncellen, en dat LAIR-2 LAIR-1-gemedieerde immuunsuppressie afgeeft.
Analyse van openbare menselijke datasets laat zien dat collagenen, LAIR-1 en LAIR-2 unieke en overlappende associaties hebben met overleving in bepaalde tumoren. We ontwierpen een dimere LAIR-2 met een functionele IgG1 Fc-staart, NC410, en toonden aan dat NC410 de humane T-celexpansie en effectorfunctie in vivo verhoogt in een muis xenogeen-graft-versus-host ziektemodel. In gehumaniseerde muistumormodellen vermindert NC410 de tumorgroei die afhankelijk is van T-cellen. Immunohistochemische analyse van humane tumoren laat zien dat NC410 bindt aan collageenrijke gebieden waar LAIR-1 + immuuncellen zijn gelokaliseerd . Onze bevindingen tonen aan dat NC410 een nieuwe strategie zou kunnen zijn voor kankerimmunotherapie voor immuun-uitgesloten tumoren.

Human Pim-3 Oncogene (PIM3) ELISA Kit |
|||
RD-PIM3-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 675.6 |
Human Pim-3 Oncogene (PIM3) ELISA Kit |
|||
RD-PIM3-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 939.6 |
Human Pim-3 Oncogene (PIM3) ELISA Kit |
|||
RDR-PIM3-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 706.8 |
Human Pim-3 Oncogene (PIM3) ELISA Kit |
|||
RDR-PIM3-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 984 |
Pim-3 Oncogene (PIM3) Antibody |
|||
20-abx131576 | Abbexa |
|
|
Pim-3 Oncogene (PIM3) Antibody |
|||
20-abx174068 | Abbexa |
|
|
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human) |
|||
4-PAN637Hu01 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3) |
Recombinant Pim-3 Oncogene (PIM3) |
|||
4-RPN637Hu01 | Cloud-Clone |
|
|
Description: Recombinant Human Pim-3 Oncogene expressed in: E.coli |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), APC |
|||
4-PAN637Hu01-APC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with APC. |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), Biotinylated |
|||
4-PAN637Hu01-Biotin | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with Biotin. |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), Cy3 |
|||
4-PAN637Hu01-Cy3 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with Cy3. |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), FITC |
|||
4-PAN637Hu01-FITC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with FITC. |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), HRP |
|||
4-PAN637Hu01-HRP | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with HRP. |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), PE |
|||
4-PAN637Hu01-PE | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with PE. |
Human Pim-3 Oncogene (PIM3) Protein |
|||
20-abx650812 | Abbexa |
|
|
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), APC-Cy7 |
|||
4-PAN637Hu01-APC-Cy7 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with APC-Cy7. |
Human Pim-3 Oncogene (PIM3) ELISA Kit |
|||
20-abx152746 | Abbexa |
|
|
Human Pim-3 Oncogene (PIM3) CLIA Kit |
|||
20-abx190020 | Abbexa |
|
|
Human Pim-3 Oncogene (PIM3)ELISA Kit |
|||
201-12-2347 | SunredBio | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Pim-3 Oncogene (PIM3) ELISA Kit |
|||
SEN637Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6227.58 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-3 Oncogene (PIM3) ELISA Kit |
|||
SEN637Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 618.04 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-3 Oncogene (PIM3) ELISA Kit |
|||
SEN637Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 831.48 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-3 Oncogene (PIM3) ELISA Kit |
|||
SEN637Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3381.66 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-3 Oncogene (PIM3) ELISA Kit |
|||
4-SEN637Hu | Cloud-Clone |
|
|
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Pim-3 Oncogene (PIM3) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Human Pim-3 Oncogene (PIM3)CLIA Kit |
|||
SCN637Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6777.36 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids. |
Human Pim-3 Oncogene (PIM3)CLIA Kit |
|||
SCN637Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 663.31 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids. |
Human Pim-3 Oncogene (PIM3)CLIA Kit |
|||
SCN637Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 896.16 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids. |
Human Pim-3 Oncogene (PIM3)CLIA Kit |
|||
SCN637Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3672.72 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids. |
Human Pim-3 Oncogene (PIM3) CLIA Kit |
|||
4-SCN637Hu | Cloud-Clone |
|
|
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3)tissue homogenates and other biological fluids |
Human Pim-3 Oncogene(PIM3)ELISA Kit |
|||
QY-E04696 | Qayee Biotechnology | 96T | EUR 433.2 |
Rat Pim-3 Oncogene(PIM3)ELISA Kit |
|||
QY-E10537 | Qayee Biotechnology | 96T | EUR 480 |
Mouse Pim-3 Oncogene(PIM3)ELISA Kit |
|||
QY-E21186 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Pim-3 Oncogene (PIM3)ELISA Kit |
|||
YLA3058HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human Pim-3 Oncogene (PIM3)ELISA Kit |
|||
YLA3058HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
ELISA kit for Human PIM3 (Pim-3 Oncogene) |
|||
ELK6491 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Pim-3 Oncogene from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Rabbit Polyclonal antibody Anti-CRBN |
|||
Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
Polyclonal Goat anti-GST p-form |
|||
GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
Serine/Threonine-Protein Kinase Pim-3 (PIM3) Antibody |
|||
20-abx241699 | Abbexa |
|
|
Serine/threonine-Protein Kinase Pim-3 (PIM3) Antibody |
|||
20-abx318212 | Abbexa |
|
|
Serine/Threonine-Protein Kinase Pim-3 (PIM3) Antibody |
|||
20-abx214125 | Abbexa |
|
|
Rabbit Pim 1 Oncogene ELISA kit |
|||
E04P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Pim 1 Oncogene ELISA kit |
|||
E04P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Pim 1 Oncogene ELISA kit |
|||
E04P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Serine/threonine-Protein Kinase Pim-3 (PIM3) Antibody (HRP) |
|||
20-abx315070 | Abbexa |
|
|
Serine/threonine-Protein Kinase Pim-3 (PIM3) Antibody (FITC) |
|||
20-abx315071 | Abbexa |
|
|
Serine/threonine-Protein Kinase Pim-3 (PIM3) Antibody (Biotin) |
|||
20-abx315072 | Abbexa |
|
|
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse) |
|||
4-PAC578Mu01 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1) |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat) |
|||
4-PAC578Ra01 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1) |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human) |
|||
4-PAP797Hu01 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2) |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse) |
|||
4-PAP797Mu01 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2) |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat) |
|||
4-PAP797Ra01 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2) |
Pim-1 Oncogene (PIM1) Antibody |
|||
abx146417-100ug | Abbexa | 100 ug | EUR 469.2 |
Pim-1 Oncogene (PIM1) Antibody |
|||
20-abx101233 | Abbexa |
|
|
Pim-1 Oncogene (PIM1) Antibody |
|||
20-abx101234 | Abbexa |
|
|
Pim-1 Oncogene (PIM1) Antibody |
|||
20-abx101235 | Abbexa |
|
|
Pim-2 Oncogene (PIM2) Antibody |
|||
20-abx101236 | Abbexa |
|
|
Pim-2 Oncogene (PIM2) Antibody |
|||
20-abx101237 | Abbexa |
|
|
Pim-2 Oncogene (PIM2) Antibody |
|||
20-abx129066 | Abbexa |
|
|
Pim-2 Oncogene (PIM2) Antibody |
|||
abx431508-200ul | Abbexa | 200 ul | EUR 460.8 |
Pim-2 Oncogene (PIM2) Antibody |
|||
abx433133-200ul | Abbexa | 200 ul | EUR 460.8 |
Pim-2 Oncogene (PIM2) Antibody |
|||
abx433134-200ul | Abbexa | 200 ul | EUR 460.8 |
Pim-1 Oncogene (PIM1) Antibody |
|||
20-abx174066 | Abbexa |
|
|
Pim-2 Oncogene (PIM2) Antibody |
|||
20-abx174067 | Abbexa |
|
|
Pim-2 Oncogene (PIM2) Antibody |
|||
abx236456-100ug | Abbexa | 100 ug | EUR 661.2 |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), APC |
|||
4-PAC578Mu01-APC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with APC. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), Biotinylated |
|||
4-PAC578Mu01-Biotin | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), Cy3 |
|||
4-PAC578Mu01-Cy3 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), FITC |
|||
4-PAC578Mu01-FITC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with FITC. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), HRP |
|||
4-PAC578Mu01-HRP | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with HRP. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), PE |
|||
4-PAC578Mu01-PE | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with PE. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), APC |
|||
4-PAC578Ra01-APC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), Biotinylated |
|||
4-PAC578Ra01-Biotin | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), Cy3 |
|||
4-PAC578Ra01-Cy3 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), FITC |
|||
4-PAC578Ra01-FITC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with FITC. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), HRP |
|||
4-PAC578Ra01-HRP | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with HRP. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), PE |
|||
4-PAC578Ra01-PE | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with PE. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), APC |
|||
4-PAP797Hu01-APC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with APC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), Biotinylated |
|||
4-PAP797Hu01-Biotin | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with Biotin. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), Cy3 |
|||
4-PAP797Hu01-Cy3 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with Cy3. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), FITC |
|||
4-PAP797Hu01-FITC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with FITC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), HRP |
|||
4-PAP797Hu01-HRP | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with HRP. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), PE |
|||
4-PAP797Hu01-PE | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with PE. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), APC |
|||
4-PAP797Mu01-APC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with APC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), Biotinylated |
|||
4-PAP797Mu01-Biotin | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with Biotin. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), Cy3 |
|||
4-PAP797Mu01-Cy3 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with Cy3. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), FITC |
|||
4-PAP797Mu01-FITC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with FITC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), HRP |
|||
4-PAP797Mu01-HRP | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with HRP. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), PE |
|||
4-PAP797Mu01-PE | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with PE. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), APC |
|||
4-PAP797Ra01-APC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with APC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), Biotinylated |
|||
4-PAP797Ra01-Biotin | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with Biotin. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), Cy3 |
|||
4-PAP797Ra01-Cy3 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with Cy3. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), FITC |
|||
4-PAP797Ra01-FITC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with FITC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), HRP |
|||
4-PAP797Ra01-HRP | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with HRP. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), PE |
|||
4-PAP797Ra01-PE | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with PE. |
Anti-PIM3 antibody |
|||
STJ119424 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis. |
Pim-1 Oncogene (PIM1) Antibody (Biotin) |
|||
20-abx272590 | Abbexa |
|
|
Pim-1 Oncogene (PIM1) Antibody (Biotin) |
|||
20-abx273089 | Abbexa |
|
|
Pim-1 Oncogene (PIM1) Antibody (Biotin) |
|||
20-abx271779 | Abbexa |
|
|
Recombinant Pim-2 Oncogene (PIM2) |
|||
4-RPP797Hu01 | Cloud-Clone |
|
|
Description: Recombinant Human Pim-2 Oncogene expressed in: E.coli |
Recombinant Pim-2 Oncogene (PIM2) |
|||
4-RPP797Mu01 | Cloud-Clone |
|
|
Description: Recombinant Mouse Pim-2 Oncogene expressed in: E.coli |
Recombinant Pim-2 Oncogene (PIM2) |
|||
4-RPP797Ra01 | Cloud-Clone |
|
|
Description: Recombinant Rat Pim-2 Oncogene expressed in: E.coli |
Recombinant Pim-1 Oncogene (PIM1) |
|||
4-RPC578Hu01 | Cloud-Clone |
|
|
Description: Recombinant Human Pim-1 Oncogene expressed in: E.coli |
Recombinant Pim-1 Oncogene (PIM1) |
|||
4-RPC578Mu01 | Cloud-Clone |
|
|
Description: Recombinant Mouse Pim-1 Oncogene expressed in: E.coli |
Recombinant Pim-1 Oncogene (PIM1) |
|||
4-RPC578Ra01 | Cloud-Clone |
|
|
Description: Recombinant Rat Pim-1 Oncogene expressed in: E.coli |
Human Serine/threonine-protein kinase pim-3 (PIM3) ELISA Kit |
|||
abx251644-96tests | Abbexa | 96 tests | EUR 801.6 |
Mouse Serine/threonine-protein kinase pim-3 (PIM3) ELISA Kit |
|||
abx520391-96tests | Abbexa | 96 tests | EUR 801.6 |
Rat Serine/threonine-protein kinase pim-3 (PIM3) ELISA Kit |
|||
abx520392-96tests | Abbexa | 96 tests | EUR 801.6 |
Rat Pim3/ Serine/threonine-protein kinase pim-3 ELISA Kit |
|||
E0762Ra | Sunlong | 1 Kit | EUR 775.2 |
Human PIM3(Serine/threonine-protein kinase pim-3) ELISA Kit |
|||
EH2293 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
Mouse Pim3/ Serine/threonine-protein kinase pim-3 ELISA Kit |
|||
E1140Mo | Sunlong | 1 Kit | EUR 758.4 |
Human PIM3/ Serine/threonine-protein kinase pim-3 ELISA Kit |
|||
E1942Hu | Sunlong | 1 Kit | EUR 726 |
Human Serine/threonine- protein kinase pim- 3, PIM3 ELISA KIT |
|||
ELI-37397h | Lifescience Market | 96 Tests | EUR 988.8 |
Mouse Serine/threonine- protein kinase pim- 3, Pim3 ELISA KIT |
|||
ELI-37973m | Lifescience Market | 96 Tests | EUR 1038 |
PIM3 Rabbit pAb |
|||
A17285-100ul | Abclonal | 100 ul | EUR 369.6 |
PIM3 Rabbit pAb |
|||
A17285-200ul | Abclonal | 200 ul | EUR 550.8 |
PIM3 Rabbit pAb |
|||
A17285-20ul | Abclonal | 20 ul | EUR 219.6 |
PIM3 Rabbit pAb |
|||
A17285-50ul | Abclonal | 50 ul | EUR 267.6 |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat) |
|||
4-PAC578Hu01 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1) |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), APC-Cy7 |
|||
4-PAC578Mu01-APC-Cy7 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with APC-Cy7. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), APC-Cy7 |
|||
4-PAC578Ra01-APC-Cy7 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC-Cy7. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), APC-Cy7 |
|||
4-PAP797Hu01-APC-Cy7 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with APC-Cy7. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), APC-Cy7 |
|||
4-PAP797Mu01-APC-Cy7 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with APC-Cy7. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), APC-Cy7 |
|||
4-PAP797Ra01-APC-Cy7 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with APC-Cy7. |
Anti-active Caspase-3 Rabbit Monoclonal Antibody |
|||
M00334-3 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Anti-active Caspase-3 Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human |
Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody |
|||
M02431-3 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Rabbit Monoclonal 14-3-3 alpha + beta Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat. |
Pim3 ELISA Kit| Mouse Serine/threonine-protein kinase pim-3 ELI |
|||
EF015888 | Lifescience Market | 96 Tests | EUR 826.8 |
Pim3 ELISA Kit| Rat Serine/threonine-protein kinase pim-3 ELISA |
|||
EF019184 | Lifescience Market | 96 Tests | EUR 826.8 |
ELISA kit for Mouse Serine/threonine-protein kinase pim-3 (PIM3) |
|||
KTE70748-48T | Abbkine | 48T | EUR 398.4 |
Description: Quantitative sandwich ELISA for measuring Mouse Serine/threonine-protein kinase pim-3 (PIM3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Mouse Serine/threonine-protein kinase pim-3 (PIM3) |
|||
KTE70748-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
Description: Quantitative sandwich ELISA for measuring Mouse Serine/threonine-protein kinase pim-3 (PIM3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Mouse Serine/threonine-protein kinase pim-3 (PIM3) |
|||
KTE70748-96T | Abbkine | 96T | EUR 646.8 |
Description: Quantitative sandwich ELISA for measuring Mouse Serine/threonine-protein kinase pim-3 (PIM3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), APC |
|||
4-PAC578Hu01-APC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), Biotinylated |
|||
4-PAC578Hu01-Biotin | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), Cy3 |
|||
4-PAC578Hu01-Cy3 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), FITC |
|||
4-PAC578Hu01-FITC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with FITC. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), HRP |
|||
4-PAC578Hu01-HRP | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with HRP. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), PE |
|||
4-PAC578Hu01-PE | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with PE. |
Anti-PIM3 (4A9) |
|||
YF-MA20163 | Abfrontier | 100 ug | EUR 435.6 |
Description: Mouse monoclonal to PIM3 |
Human Pim-2 Oncogene (PIM2) Protein |
|||
20-abx168058 | Abbexa |
|
|
Human Pim-1 Oncogene (PIM1) Protein |
|||
20-abx068546 | Abbexa |
|
|
Rat Pim-1 Oncogene (PIM1) Protein |
|||
20-abx068547 | Abbexa |
|
|
Mouse Pim-1 Oncogene (PIM1) Protein |
|||
20-abx068548 | Abbexa |
|
|
Mouse Pim-2 Oncogene (PIM2) Protein |
|||
20-abx068549 | Abbexa |
|
|
Rat Pim-2 Oncogene (PIM2) Protein |
|||
20-abx068550 | Abbexa |
|
|
Goat Pim 1 Oncogene ELISA kit |
|||
E06P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Goat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Pim 1 Oncogene ELISA kit |
|||
E06P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Goat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Pim 1 Oncogene ELISA kit |
|||
E06P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Goat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Pim 1 Oncogene ELISA kit |
|||
E03P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Mouse Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Pim 1 Oncogene ELISA kit |
|||
E03P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Mouse Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Pim 1 Oncogene ELISA kit |
|||
E03P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Mouse Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Pim 1 Oncogene ELISA kit |
|||
E08P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Canine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Pim 1 Oncogene ELISA kit |
|||
E08P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Canine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Pim 1 Oncogene ELISA kit |
|||
E08P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Canine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Pim 1 Oncogene ELISA kit |
|||
E09P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Monkey Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Pim 1 Oncogene ELISA kit |
|||
E09P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Monkey Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Pim 1 Oncogene ELISA kit |
|||
E09P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Monkey Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Pim 1 Oncogene ELISA kit |
|||
E02P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Pim 1 Oncogene ELISA kit |
|||
E02P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Pim 1 Oncogene ELISA kit |
|||
E02P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Pim 1 Oncogene ELISA kit |
|||
E01P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Human Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Pim 1 Oncogene ELISA kit |
|||
E01P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Human Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Pim 1 Oncogene ELISA kit |
|||
E01P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Human Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Pim 1 Oncogene ELISA kit |
|||
E07P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Porcine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Pim 1 Oncogene ELISA kit |
|||
E07P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Porcine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Pim 1 Oncogene ELISA kit |
|||
E07P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Porcine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Anti-Actin Rabbit Monoclonal Antibody |
|||
M02014-3 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Rabbit Monoclonal Actin Antibody. Validated in IF, WB and tested in Human, Mouse, Rat. |
Anti-Ubiquitin Rabbit Monoclonal Antibody |
|||
M02848-3 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Rabbit Monoclonal Ubiquitin Antibody. Validated in Flow Cytometry, IF, IHC, ICC, WB and tested in Human, Mouse, Rat. |
Anti-PARP Rabbit Monoclonal Antibody |
|||
M00122-3 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Rabbit Monoclonal PARP Antibody. Validated in IF, WB and tested in Human, Mouse, Rat. |
Anti-p21 Rabbit Monoclonal Antibody |
|||
M00145-3 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Rabbit Monoclonal p21 Antibody. Validated in IP, IF, IHC, WB and tested in Human. |
Anti-PAX6 Rabbit Monoclonal Antibody |
|||
M00273-3 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Rabbit Monoclonal PAX6 Antibody. Validated in IF, WB and tested in Human, Mouse, Rat. |
PIM3 Antibody |
|||
37701-100ul | SAB | 100ul | EUR 302.4 |
PIM3 Antibody |
|||
1-CSB-PA771444LA01HU | Cusabio |
|
|
Description: A polyclonal antibody against PIM3. Recognizes PIM3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
PIM3 Antibody |
|||
1-CSB-PA968784 | Cusabio |
|
|
Description: A polyclonal antibody against PIM3. Recognizes PIM3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100 |
PIM3 Antibody |
|||
DF14059 | Affbiotech | 100ul | EUR 420 |
PIM3 Antibody |
|||
1-CSB-PA027525 | Cusabio |
|
|
Description: A polyclonal antibody against PIM3. Recognizes PIM3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200 |
Anti-Pim-1 antibody |
|||
STJ95093 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Pim-1. |
Immunologische identificatie en karakterisering van het capside-scaffold- eiwit gecodeerd door UL26.5 van Herpes Simplex Virus Kind 2
Herpes simplex-virus kind 2 (HSV2), een pathogeen dat genitale herpeslaesies veroorzaakt, interfereert met het immuunsysteem van de gastheer through verschillende bekende en onbekende mechanismen. Dit virus is gebruikt om de virale antigene samenstelling te bestuderen. Herstellend serum van HSV2-geïnfecteerde patiënten werd gebruikt om virale antigenen te identificeren through 2-D-eiwitelektroforese en immunoblotting. Het serum herkende voornamelijk verschillende capside-scaffold-eiwitten die worden gecodeerd door gen UL26.5, voornamelijk ICP35 .
Van dit eiwit is voornamelijk gemeld dat het tijdelijk functioneert in virale assemblage, maar wordt niet tot expressie gebracht in rijpe virusdeeltjes. Verdere immunologische research suggereerden dat dit eiwit specifieke antilichaam- en cytotoxische T-lymfocyten (CTL)-reacties opwekt bij muizen, maar deze reacties resulteren niet in een klinisch beschermend impact als reactie op HSV2-uitdaging . De gegevens suggereerden dat immunodominantie van ICP35 zou kunnen worden gebruikt om een geïntegreerd antigeen te ontwerpen met andere virale glycoproteïnen.
Auto-immuunkenmerken van veroudering en leeftijdsgerelateerde neurodegeneratieve ziekten zijn geassocieerd met hersenfunctie en ribosomale eiwitten
Biologische veroudering is een complicated proces dat wordt gekenmerkt door een verminderde functie van cellen en weefsels, inclusief die van het immuunsysteem. Als gevolg hiervan beïnvloedt veroudering de expressie en ontwikkeling van auto-antilichamen en autoreactieve T-cellen, die in hun geheel kunnen worden gezien als het auto-immunoom van een individu. In deze studie hebben we geanalyseerd of units van auto-immuunkenmerken geassocieerd zijn met specifieke fenotypes die auto-immunomische kenmerken vormen die verband houden met leeftijd en neurodegeneratieve ziekten. De auto-antilichaamprofielgegevens van gezonde proefpersonen en patiënten uit de GEO-database werden gebruikt om auto-immunomische kenmerken van veroudering en drie neurodegeneratieve ziekten te onderzoeken, waaronder de ziekte van Parkinson (PD), de ziekte van Alzheimer (AD) en a number of sclerose (MS).
Onze resultaten tonen aan dat de auto-immunomische signatuur van veroudering wordt gekenmerkt door een gegolfde toename van IgG-auto-antilichamen geassocieerd met leren en gedrag en een consistente toename van IgG-auto-antilichamen gerelateerd aan ribosoom en translatie, en de auto-immunomische signatuur van veroudering is ook geassocieerd met leeftijdsgebonden neurodegeneratieve ziekten. Intrigerend genoeg identificeerde Differential Expression-Sliding Window Evaluation (DE-SWAN) drie golven van veranderingen van auto-antilichamen tijdens veroudering op een leeftijd van respectievelijk 30, 50 en 62 jaar. Bovendien, IgG auto-antilichamen, in het bijzonderdie tegen ribosomale eiwitten, zouden kunnen worden gebruikt als voorspellingsmarkers voor veroudering en leeftijdsgerelateerde neurodegeneratieve ziekten. Daarom onthult deze studie voor het eerst uitgebreide auto-immunomische handtekeningen voor veroudering en leeftijdsgerelateerde neurodegeneratieve ziekten.
Identificatie van 7 000-9 000 eiwitten uit cellijnen en weefsels door Single-Shot Microflow LC-MS/MS
Een huidige pattern in proteomics is het verkrijgen van gegevens in een “single-shot” door LC-MS/MS omdat het workflows vereenvoudigt en een betere doorvoer en kwantitatieve nauwkeurigheid belooft dan schema’s die uitgebreide steekproeffractionering omvatten. Single-shot benaderingen kunnen echter final hebben van beperkte proteoomdekking wanneer ze worden uitgevoerd door data-afhankelijke acquisitie (ssDDA) op nanoflow LC-systemen. Voor toepassingen waar monsterhoeveelheden niet schaars zijn, toont deze studie aan dat een hoge proteoomdekking kan worden verkregen met behulp van een microflow LC-MS/MS-systeem met een 1 mm id × 150 mm kolom, met een stroomsnelheid van 50 μL/min en gekoppeld naar een Orbitrap HF-X massaspectrometer.
De resultaten tonen het identificeren van eiwitten -9 000 50 ug eiwit verteren van Arabidopsis wortels 7 500 uit muizen thymus en 7 300 uit humane borstkankercellen in three uur analysetijd in een enkele run. Het dynamische bereik van eiwitkwantificering gemeten door de iBAQ-benadering besloeg 5 ordes van grootte en replica-analyse toonde aan dat de mediane variatiecoëfficiënt lager was dan 20%. Samen laat deze studie zien dat ssDDA door μLC-MS/MS een robuuste methode is voor uitgebreide en grootschalige proteoomanalyse en die in de toekomst verder kan worden uitgebreid tot snellere chromatografie en data-onafhankelijke acquisitiebenaderingen.̀.
FOS Like Antigen 1 (FOSL1) Antibody |
|||
20-abx121479 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Antibody |
|||
20-abx129796 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Antibody |
|||
20-abx007249 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Antibody |
|||
20-abx004113 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Antibody |
|||
20-abx013397 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Antibody |
|||
abx332630-100ul | Abbexa | 100 ul | EUR 510 |
FOS Like Antigen 1 (FOSL1) Antibody |
|||
abx430072-200ul | Abbexa | 200 ul | EUR 460.8 |
FOS Like Antigen 1 (FOSL1) Antibody |
|||
20-abx320611 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Antibody |
|||
20-abx328628 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Antibody |
|||
20-abx242558 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Antibody |
|||
20-abx242564 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Antibody |
|||
abx215451-100ug | Abbexa | 100 ug | EUR 526.8 |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Rat) |
|||
4-PAJ089Ra01 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat FOS Like Antigen 1 (FOSL1) |
Recombinant FOS Like Antigen 1 (FOSL1) |
|||
4-RPJ089Hu01 | Cloud-Clone |
|
|
Description: Recombinant Human FOS Like Antigen 1 expressed in: E.coli |
Recombinant FOS Like Antigen 1 (FOSL1) |
|||
4-RPJ089Mu01 | Cloud-Clone |
|
|
Description: Recombinant Mouse FOS Like Antigen 1 expressed in: E.coli |
Recombinant FOS Like Antigen 1 (FOSL1) |
|||
4-RPJ089Ra01 | Cloud-Clone |
|
|
Description: Recombinant Rat FOS Like Antigen 1 expressed in: E.coli |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Rat) |
|||
4-PAJ089Hu01 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Rat FOS Like Antigen 1 (FOSL1) |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Mouse) |
|||
4-PAJ089Mu01 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse FOS Like Antigen 1 (FOSL1) |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Rat), APC |
|||
4-PAJ089Ra01-APC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with APC. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Rat), Biotinylated |
|||
4-PAJ089Ra01-Biotin | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with Biotin. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Rat), Cy3 |
|||
4-PAJ089Ra01-Cy3 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with Cy3. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Rat), FITC |
|||
4-PAJ089Ra01-FITC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with FITC. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Rat), HRP |
|||
4-PAJ089Ra01-HRP | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with HRP. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Rat), PE |
|||
4-PAJ089Ra01-PE | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with PE. |
Human FOS Like Antigen 1 (FOSL1) Protein |
|||
20-abx066690 | Abbexa |
|
|
Rat FOS Like Antigen 1 (FOSL1) Protein |
|||
20-abx066691 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Blocking Peptide |
|||
20-abx162434 | Abbexa |
|
|
Mouse FOS Like Antigen 1 (FOSL1) Protein |
|||
20-abx167489 | Abbexa |
|
|
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Rat), APC |
|||
4-PAJ089Hu01-APC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with APC. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Rat), Biotinylated |
|||
4-PAJ089Hu01-Biotin | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with Biotin. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Rat), Cy3 |
|||
4-PAJ089Hu01-Cy3 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with Cy3. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Rat), FITC |
|||
4-PAJ089Hu01-FITC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with FITC. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Rat), HRP |
|||
4-PAJ089Hu01-HRP | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with HRP. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Rat), PE |
|||
4-PAJ089Hu01-PE | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Rat FOS Like Antigen 1 (FOSL1). This antibody is labeled with PE. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Mouse), APC |
|||
4-PAJ089Mu01-APC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse FOS Like Antigen 1 (FOSL1). This antibody is labeled with APC. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Mouse), Biotinylated |
|||
4-PAJ089Mu01-Biotin | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse FOS Like Antigen 1 (FOSL1). This antibody is labeled with Biotin. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Mouse), Cy3 |
|||
4-PAJ089Mu01-Cy3 | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse FOS Like Antigen 1 (FOSL1). This antibody is labeled with Cy3. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Mouse), FITC |
|||
4-PAJ089Mu01-FITC | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse FOS Like Antigen 1 (FOSL1). This antibody is labeled with FITC. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Mouse), HRP |
|||
4-PAJ089Mu01-HRP | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse FOS Like Antigen 1 (FOSL1). This antibody is labeled with HRP. |
FOS Like Antigen 1 (FOSL1) Polyclonal Antibody (Human, Mouse), PE |
|||
4-PAJ089Mu01-PE | Cloud-Clone |
|
|
Description: A Rabbit polyclonal antibody against Human, Mouse FOS Like Antigen 1 (FOSL1). This antibody is labeled with PE. |