Snel opkomende SARS-CoV-2 B.1.1.7 sub-lijn in de Verenigde Staten van Amerika met spike eiwit D178H en membraan eiwit V70L mutaties
Samenvatting De SARS-CoV-2 B.1.1.7-afstamming is zeer besmettelijk en was vanaf april 2021 goed voor 92% van de COVID-19-gevallen in Europa en 59% van de COVID-19-gevallen in de VS. Het wordt gedefinieerd door de N501Y-mutatie in het receptorbindende domein (RBD) van het Spike (S)-eiwit en enkele andere mutaties. Deze omvatten twee mutaties in het N-terminale domein (NTD)van het S-eiwit, HV69-70del en Y144del (ook bekend als Y145del vanwege de aanwezigheid van tyrosine op beide posities). We hebben onlangs verschillende opkomende SARS-CoV-2-varianten van problemen geïdentificeerd, gekenmerkt door membraan (M) -eiwitmutaties, waaronder I82T en V70L. We identificeren nu een sublijn van B.1.1.7 die ontstond door opeenvolgende acquisities van M:V70L in november 2020, gevolgd door een nieuwe S:D178H-mutatie die voor het eerst werd waargenomen start februari 2021.
Het proportion B.1.1.7-isolaten in de VS dat tot deze sublijn behoort, is gestegen van 0,15% in februari 2021 tot 1,8% in april 2021. Tot op heden lijkt deze sublijn specifiek voor de VS te zijn met gemelde gevallen in 31 staten, waaronder Hawaï. Vanaf april 2021 vormde het 36,8% van alle B.1.1.7-isolaten in Washington. Fylogenetische analyse en transmissie-inferentie met Nextstrain suggereert dat deze sublijn waarschijnlijk afkomstig is uit Californië of Washington . Structurele analyse onthulde dat de S:D178H-mutatie zich in de NTD van het S-eiwit bevindt en dicht bij twee andere kenmerkende mutaties van B.1.1.7, HV69-70del en Y144del. Het is aan het oppervlak blootgesteld en kan de tertiaire configuratie of toegankelijkheid van NTD veranderen, en heeft dus het potentieel om neutralisatie door NTD-gerichte antilichamen te beïnvloeden.
Snelle driedimensionale vormbepaling van bolvormige eiwitten door capillaire elektroforese van mobiliteit en natieve massaspectrometrie
Gevestigde proteomics-methoden met hoge doorvoer bieden beperkte informatie over de stereostructuren van eiwitten. Traditionele technologieën voor het bepalen van de eiwitstructuur vereisen doorgaans moeizame stappen en kunnen niet op een high-throughput-manier worden uitgevoerd. Hier rapporteren we een nieuwe medium throughput-methode door mobiliteit capillaire elektroforese (MCE) en native massaspectrometrie (MS) te combineren voor de 3-dimensionale (3D) vormbepaling van bolvormige eiwitten in de vloeibare fase , die zowel de geometrische structuur als de moleculaire massa-informatie van eiwitten.
Er werd een theorie ontwikkeld om de hydrodynamische straal van ionen en de verdeling van de ladingstoestand in het natieve massaspectrum te correleren met geometrische eiwitparameters, waardoor een lage-resolutiestructuur (vorm) van het eiwit kon worden bepaald. Onze testgegevens van 11 verschillende globulaire eiwitten toonden aan dat deze benadering ons in staat stelt om de vormen van individuele eiwitten, eiwitcomplexen en eiwitten in een mengsel te bepalen en om veranderingen in eiwitconformatie te volgen. Naast het verstrekken van aanvullende informatie over de eiwitstructuur en het vermogen om mengsels te analyseren, is deze op MCE en native MS gebaseerde methode snel in snelheid en laag in monsterconsumptie, waardoor deze mogelijk toepasbaar is in top-down proteomics en structurele biologie voor intacte globulaire eiwit- of eiwitcomplexanalyse.

Human Pim-2 Oncogene (PIM2) ELISA Kit |
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RD-PIM2-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 675.6 |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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RD-PIM2-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 939.6 |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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RDR-PIM2-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 706.8 |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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RDR-PIM2-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 984 |
Pim-2 Oncogene (PIM2) Antibody |
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20-abx101236 | Abbexa |
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Pim-2 Oncogene (PIM2) Antibody |
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20-abx101237 | Abbexa |
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Pim-2 Oncogene (PIM2) Antibody |
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20-abx174067 | Abbexa |
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Pim-2 Oncogene (PIM2) Antibody |
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20-abx129066 | Abbexa |
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Pim-2 Oncogene (PIM2) Antibody |
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abx431508-200ul | Abbexa | 200 ul | EUR 460.8 |
Pim-2 Oncogene (PIM2) Antibody |
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abx433133-200ul | Abbexa | 200 ul | EUR 460.8 |
Pim-2 Oncogene (PIM2) Antibody |
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abx433134-200ul | Abbexa | 200 ul | EUR 460.8 |
Pim-2 Oncogene (PIM2) Antibody |
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abx236456-100ug | Abbexa | 100 ug | EUR 661.2 |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human) |
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4-PAP797Hu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2) |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse) |
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4-PAP797Mu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2) |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat) |
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4-PAP797Ra01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2) |
Recombinant Pim-2 Oncogene (PIM2) |
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4-RPP797Hu01 | Cloud-Clone |
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Description: Recombinant Human Pim-2 Oncogene expressed in: E.coli |
Recombinant Pim-2 Oncogene (PIM2) |
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4-RPP797Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Pim-2 Oncogene expressed in: E.coli |
Recombinant Pim-2 Oncogene (PIM2) |
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4-RPP797Ra01 | Cloud-Clone |
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Description: Recombinant Rat Pim-2 Oncogene expressed in: E.coli |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), APC |
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4-PAP797Hu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with APC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), Biotinylated |
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4-PAP797Hu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with Biotin. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), Cy3 |
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4-PAP797Hu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with Cy3. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), FITC |
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4-PAP797Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with FITC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), HRP |
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4-PAP797Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with HRP. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), PE |
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4-PAP797Hu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with PE. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), APC |
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4-PAP797Mu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with APC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), Biotinylated |
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4-PAP797Mu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with Biotin. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), Cy3 |
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4-PAP797Mu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with Cy3. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), FITC |
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4-PAP797Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with FITC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), HRP |
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4-PAP797Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with HRP. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), PE |
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4-PAP797Mu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with PE. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), APC |
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4-PAP797Ra01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with APC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), Biotinylated |
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4-PAP797Ra01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with Biotin. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), Cy3 |
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4-PAP797Ra01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with Cy3. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), FITC |
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4-PAP797Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with FITC. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), HRP |
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4-PAP797Ra01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with HRP. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), PE |
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4-PAP797Ra01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with PE. |
Mouse Pim-2 Oncogene (PIM2) Protein |
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20-abx068549 | Abbexa |
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Rat Pim-2 Oncogene (PIM2) Protein |
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20-abx068550 | Abbexa |
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Human Pim-2 Oncogene (PIM2) Protein |
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20-abx168058 | Abbexa |
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Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human), APC-Cy7 |
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4-PAP797Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2). This antibody is labeled with APC-Cy7. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse), APC-Cy7 |
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4-PAP797Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2). This antibody is labeled with APC-Cy7. |
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat), APC-Cy7 |
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4-PAP797Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2). This antibody is labeled with APC-Cy7. |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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20-abx152745 | Abbexa |
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Human Pim-2 Oncogene (PIM2) CLIA Kit |
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20-abx190021 | Abbexa |
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Human Pim-2 Oncogene (PIM2) ELISA Kit |
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201-12-2346 | SunredBio | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human Pim-2 Oncogene(PIM2)ELISA Kit |
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QY-E04697 | Qayee Biotechnology | 96T | EUR 433.2 |
Rat Pim-2 Oncogene(PIM2))ELISA Kit |
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QY-E10538 | Qayee Biotechnology | 96T | EUR 480 |
Mouse Pim-2 Oncogene(PIM2))ELISA Kit |
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QY-E21185 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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SEP797Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6227.58 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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SEP797Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 618.04 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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SEP797Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 831.48 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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SEP797Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3381.66 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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4-SEP797Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Pim-2 Oncogene (PIM2) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Human Pim-2 Oncogene (PIM2)CLIA Kit |
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SCP797Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 6777.36 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-2 Oncogene (PIM2)CLIA Kit |
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SCP797Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 663.31 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-2 Oncogene (PIM2)CLIA Kit |
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SCP797Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 896.16 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-2 Oncogene (PIM2)CLIA Kit |
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SCP797Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3672.72 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-2 Oncogene (PIM2) in serum, plasma, tissue homogenates and other biological fluids. |
Human Pim-2 Oncogene (PIM2) CLIA Kit |
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4-SCP797Hu | Cloud-Clone |
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Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Pim-2 Oncogene (PIM2)Serum, plasma, tissue homogenates and other biological fluids |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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YLA3057HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human Pim-2 Oncogene (PIM2) ELISA Kit |
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YLA3057HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
ELISA kit for Human PIM2 (Pim-2 Oncogene) |
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ELK6492 | ELK Biotech | 1 plate of 96 wells | EUR 518.4 |
Description: A sandwich ELISA kit for detection of Pim-2 Oncogene from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
Rabbit Polyclonal antibody Anti-CRBN |
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Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
Serine/Threonine-Protein Kinase Pim-2 (PIM2) Antibody |
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abx033773-400ul | Abbexa | 400 ul | EUR 627.6 |
Serine/Threonine-Protein Kinase Pim-2 (PIM2) Antibody |
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abx033773-80l | Abbexa | 80 µl | EUR 343.2 |
Serine/Threonine-Protein Kinase Pim-2 (PIM2) Antibody |
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20-abx212237 | Abbexa |
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Serine/Threonine-Protein Kinase Pim-2 (PIM2) Antibody |
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20-abx302167 | Abbexa |
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Serine/Threonine-Protein Kinase Pim-2 (PIM2) Antibody |
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20-abx322889 | Abbexa |
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Serine/Threonine-Protein Kinase Pim-2 (PIM2) Antibody |
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20-abx212644 | Abbexa |
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Polyclonal Goat anti-GST μ-form |
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GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
Rabbit Pim 1 Oncogene ELISA kit |
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E04P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Pim 1 Oncogene ELISA kit |
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E04P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Pim 1 Oncogene ELISA kit |
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E04P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Serine/Threonine-Protein Kinase Pim-2 (PIM2) Antibody (HRP) |
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20-abx314668 | Abbexa |
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Serine/Threonine-Protein Kinase Pim-2 (PIM2) Antibody (FITC) |
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20-abx314669 | Abbexa |
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Serine/Threonine-Protein Kinase Pim-2 (PIM2) Antibody (Biotin) |
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20-abx314670 | Abbexa |
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Serine/Threonine-Protein Kinase Pim-2 (PIM2) Protein |
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20-abx262771 | Abbexa |
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Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human) |
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4-PAN637Hu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3) |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse) |
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4-PAC578Mu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1) |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat) |
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4-PAC578Ra01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1) |
Pim-1 Oncogene (PIM1) Antibody |
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20-abx101233 | Abbexa |
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Pim-1 Oncogene (PIM1) Antibody |
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20-abx101234 | Abbexa |
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Pim-1 Oncogene (PIM1) Antibody |
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20-abx101235 | Abbexa |
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Pim-1 Oncogene (PIM1) Antibody |
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20-abx174066 | Abbexa |
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Pim-3 Oncogene (PIM3) Antibody |
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20-abx174068 | Abbexa |
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Pim-1 Oncogene (PIM1) Antibody |
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abx146417-100ug | Abbexa | 100 ug | EUR 469.2 |
Pim-3 Oncogene (PIM3) Antibody |
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20-abx131576 | Abbexa |
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Anti-Pim-2 antibody |
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STJ95094 | St John's Laboratory | 200 µl | EUR 236.4 |
Description: Rabbit polyclonal to Pim-2. |
Pim-2 Polyclonal Antibody |
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ABP53587-003ml | Abbkine | 0.03ml | EUR 189.6 |
Description: A polyclonal antibody for detection of Pim-2 from Human. This Pim-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Pim-2 |
Pim-2 Polyclonal Antibody |
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ABP53587-01ml | Abbkine | 0.1ml | EUR 346.8 |
Description: A polyclonal antibody for detection of Pim-2 from Human. This Pim-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Pim-2 |
Pim-2 Polyclonal Antibody |
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ABP53587-02ml | Abbkine | 0.2ml | EUR 496.8 |
Description: A polyclonal antibody for detection of Pim-2 from Human. This Pim-2 antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human Pim-2 |
Pim-2 Polyclonal Antibody |
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ES4586-100ul | ELK Biotech | 100ul | EUR 334.8 |
Description: A Rabbit Polyclonal antibody against Pim-2 from Human. This antibody is tested and validated for IHC, WB, ELISA |
Pim-2 Polyclonal Antibody |
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ES4586-50ul | ELK Biotech | 50ul | EUR 248.4 |
Description: A Rabbit Polyclonal antibody against Pim-2 from Human. This antibody is tested and validated for IHC, WB, ELISA |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), APC |
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4-PAN637Hu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with APC. |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), Biotinylated |
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4-PAN637Hu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with Biotin. |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), Cy3 |
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4-PAN637Hu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with Cy3. |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), FITC |
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4-PAN637Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with FITC. |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), HRP |
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4-PAN637Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with HRP. |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), PE |
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4-PAN637Hu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with PE. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), APC |
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4-PAC578Mu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with APC. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), Biotinylated |
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4-PAC578Mu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), Cy3 |
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4-PAC578Mu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), FITC |
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4-PAC578Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with FITC. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), HRP |
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4-PAC578Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with HRP. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), PE |
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4-PAC578Mu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with PE. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), APC |
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4-PAC578Ra01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), Biotinylated |
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4-PAC578Ra01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), Cy3 |
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4-PAC578Ra01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), FITC |
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4-PAC578Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with FITC. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), HRP |
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4-PAC578Ra01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with HRP. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), PE |
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4-PAC578Ra01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with PE. |
Pim-1 Oncogene (PIM1) Antibody (Biotin) |
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20-abx271779 | Abbexa |
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Pim-1 Oncogene (PIM1) Antibody (Biotin) |
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20-abx272590 | Abbexa |
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Pim-1 Oncogene (PIM1) Antibody (Biotin) |
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20-abx273089 | Abbexa |
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anti- PIM2 antibody |
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FNab06456 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against PIM2 |
Anti-PIM2 antibody |
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PAab06456 | Lifescience Market | 100 ug | EUR 494.4 |
Anti-PIM2 antibody |
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STJ72541 | St John's Laboratory | 100 µg | EUR 430.8 |
Anti-PIM2 antibody |
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STJ118061 | St John's Laboratory | 100 µl | EUR 332.4 |
Mouse Pim2/ Serine/threonine-protein kinase pim-2 ELISA Kit |
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E1139Mo | Sunlong | 1 Kit | EUR 685.2 |
Human Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit |
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abx573365-96tests | Abbexa | 96 tests | EUR 801.6 |
Mouse Serine/threonine-protein kinase pim-2 (PIM2) ELISA Kit |
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abx555894-96tests | Abbexa | 96 tests | EUR 801.6 |
Human PIM2(Serine/threonine-protein kinase pim-2) ELISA Kit |
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EH11135 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml |
Mouse Serine/threonine- protein kinase pim- 2, Pim2 ELISA KIT |
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ELI-43622m | Lifescience Market | 96 Tests | EUR 1038 |
Human Serine/threonine- protein kinase pim- 2, PIM2 ELISA KIT |
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ELI-36413h | Lifescience Market | 96 Tests | EUR 988.8 |
Recombinant Pim-1 Oncogene (PIM1) |
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4-RPC578Hu01 | Cloud-Clone |
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Description: Recombinant Human Pim-1 Oncogene expressed in: E.coli |
Recombinant Pim-1 Oncogene (PIM1) |
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4-RPC578Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Pim-1 Oncogene expressed in: E.coli |
Recombinant Pim-1 Oncogene (PIM1) |
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4-RPC578Ra01 | Cloud-Clone |
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Description: Recombinant Rat Pim-1 Oncogene expressed in: E.coli |
Recombinant Pim-3 Oncogene (PIM3) |
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4-RPN637Hu01 | Cloud-Clone |
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Description: Recombinant Human Pim-3 Oncogene expressed in: E.coli |
anti-PIM2 |
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YF-PA17388 | Abfrontier | 50 ul | EUR 435.6 |
Description: Mouse polyclonal to Anti-PIM2 |
anti-PIM2 |
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YF-PA17389 | Abfrontier | 50 ug | EUR 435.6 |
Description: Mouse polyclonal to Anti-PIM2 |
anti-PIM2 |
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YF-PA17390 | Abfrontier | 100 ul | EUR 483.6 |
Description: Rabbit polyclonal to Anti-PIM2 |
anti-PIM2 |
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YF-PA17391 | Abfrontier | 100 ug | EUR 483.6 |
Description: Rabbit polyclonal to Anti-PIM2 |
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), APC-Cy7 |
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4-PAN637Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with APC-Cy7. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat) |
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4-PAC578Hu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1) |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), APC-Cy7 |
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4-PAC578Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with APC-Cy7. |
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), APC-Cy7 |
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4-PAC578Ra01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC-Cy7. |
PIM2 Rabbit pAb |
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A15598-100ul | Abclonal | 100 ul | EUR 369.6 |
PIM2 Rabbit pAb |
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A15598-200ul | Abclonal | 200 ul | EUR 550.8 |
PIM2 Rabbit pAb |
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A15598-20ul | Abclonal | 20 ul | EUR 219.6 |
PIM2 Rabbit pAb |
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A15598-50ul | Abclonal | 50 ul | EUR 267.6 |
ELISA kit for Mouse Serine/threonine-protein kinase pim-2 (PIM2) |
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KTE70749-48T | Abbkine | 48T | EUR 398.4 |
Description: Quantitative sandwich ELISA for measuring Mouse Serine/threonine-protein kinase pim-2 (PIM2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Mouse Serine/threonine-protein kinase pim-2 (PIM2) |
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KTE70749-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
Description: Quantitative sandwich ELISA for measuring Mouse Serine/threonine-protein kinase pim-2 (PIM2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Mouse Serine/threonine-protein kinase pim-2 (PIM2) |
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KTE70749-96T | Abbkine | 96T | EUR 646.8 |
Description: Quantitative sandwich ELISA for measuring Mouse Serine/threonine-protein kinase pim-2 (PIM2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
Anti-Bcl-2 Rabbit Monoclonal Antibody |
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M00040-2 | BosterBio | 100ug/vial | EUR 476.4 |
Description: Rabbit Monoclonal Bcl-2 Antibody. Validated in IF, WB and tested in Human, Mouse. |
Anti-PIM2 (Internal) antibody |
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STJ70829 | St John's Laboratory | 100 µg | EUR 430.8 |
Human Pim 1 Oncogene ELISA kit |
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E01P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Human Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Pim 1 Oncogene ELISA kit |
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E01P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Human Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Pim 1 Oncogene ELISA kit |
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E01P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Human Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Pim 1 Oncogene ELISA kit |
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E06P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Goat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Pim 1 Oncogene ELISA kit |
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E06P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Goat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Pim 1 Oncogene ELISA kit |
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E06P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Goat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Pim 1 Oncogene ELISA kit |
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E08P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Canine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Pim 1 Oncogene ELISA kit |
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E08P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Canine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Pim 1 Oncogene ELISA kit |
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E08P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Canine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Pim 1 Oncogene ELISA kit |
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E07P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Porcine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Pim 1 Oncogene ELISA kit |
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E07P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Porcine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Pim 1 Oncogene ELISA kit |
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E07P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Porcine Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Pim 1 Oncogene ELISA kit |
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E03P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Mouse Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Pim 1 Oncogene ELISA kit |
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E03P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Mouse Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Pim 1 Oncogene ELISA kit |
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E03P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Mouse Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Pim 1 Oncogene ELISA kit |
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E02P0753-192T | BlueGene | 192 tests | EUR 1524 |
Description: A competitive ELISA for quantitative measurement of Rat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Pim 1 Oncogene ELISA kit |
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E02P0753-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
Description: A competitive ELISA for quantitative measurement of Rat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Pim 1 Oncogene ELISA kit |
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E02P0753-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
Description: A competitive ELISA for quantitative measurement of Rat Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Pim-1 Oncogene (PIM1) Protein |
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20-abx068546 | Abbexa |
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Rat Pim-1 Oncogene (PIM1) Protein |
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20-abx068547 | Abbexa |
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Mouse Pim-1 Oncogene (PIM1) Protein |
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20-abx068548 | Abbexa |
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Snelle oppervlakte-immobilisatie van natieve eiwitten door katalysatorvrije amino-yn-klik-bioconjugatie
Oppervlakte-immobilisatie biedt een nuttig platform voor biosensing, screening van geneesmiddelen, weefselengineering en andere chemische en biologische toepassingen. Echter , sommige van de gebruikte reacties zijn inefficiënt en / of ingewikkeld, hun toepassingen te beperken in immobilisatie. Hierin gebruiken we een spontane en katalysatorvrije amino-yn-klik bioconjugatie om geactiveerde met ethynylgroep gefunctionaliseerde oppervlakken te genereren voor snelle immobilisatie van inheemse eiwitten en cellen.
Biomoleculen, zoals runderserumalbumine (BSA) , humaan IgG en een peptide van C(RGDfK), konden in slechts 30 minuten covalent op de oppervlakken worden geïmmobiliseerd. Met identify de biologische activiteit van de verankerde biomoleculen blijft intact, wat wordt geverifieerd door het efficiënt vastleggen van doelantilichamen en cellen uit de bulkoplossingen. Deze strategie vertegenwoordigt een alternatief voor zeer efficiënte biofunctionalisatie van het oppervlak.
Over de bindingsreactie van loratadine met humaan serum acute fase- eiwit alfa-1-zuur glyco- eiwit
Loratadine is een belangrijk anti-allergisch medicijn. Het is een antihistaminicum van de tweede generatie dat wordt gebruikt voor de behandeling van allergische rhinitis, hooikoorts en urticaria. Humaan alfa-1-zuur-glycoproteïne (AG) in serum is een belangrijk eiwit in de acute fase en de serumconcentratie ervan blijkt te stijgen bij ontsteking en acute respons. De bindingsinteractie tussen loratadine en AG wordt bestudeerd met behulp van spectroscopie en moleculaire koppeltechnieken. De resultaten verkregen uit fluorescentie- uitdovingsexperimenten toonden aan dat de fluorescentie-intensiteit van AG wordt gedoofd door loratadine. Loratadine bleek AG binden aan de bindingsconstante van ≈10 4 bij 298 Okay.
De vrije energieverandering van Gibb bleek negatief te zijn voor de interactie van loratadine met AG, wat aangeeft dat het bindingsproces spontaan is. Binding van loratadine met AG induceerde geordende structuren in het eiwit. Waterstofbinding en hydrofobe interacties waren de belangrijkste bindingskrachten tussen AG-loratadine, zoals blijkt uit moleculaire koppelingsresultaten. Deze studie suggereert het belang van het ruimtelijk binden van anti-allergische geneesmiddelen aan AG bij ziekten waarbij de plasmaconcentratie van AG vele malen toeneemt en interactie met dit eiwit vital wordt. Deze studie zal helpen bij het ontwerpen van de medicijndosering en dienovereenkomstige aanpassing om een optimaal behandelresultaat te bereiken. Gecommuniceerd door Ramaswamy H. Sarma.
Gerichte Protein Degradatie door middel van Quick optogenetische Activering en de toepassing ervan op de beheersing van de Cell Signaling
De ontwikkeling van methodologieën voor optisch getriggerde eiwitafbraak maakt de studie mogelijk van dynamische eiwitfuncties, zoals die welke betrokken zijn bij celsignalering , die moeilijk te onderzoeken zijn met traditionele genetische technieken. Hier beschrijven we het ontwerp en de implementatie van een nieuw lichtgecontroleerd peptide-degron dat N-end-pathway-degradatie aan zijn eiwitdoelwit verleent. De degron omvat een fotogekooid N-terminaal aminozuur en een lysinerijke linker met 13 residu’s. Door het N-terminale residu te kooien , waren we in staat om de herkenning van N-degron door een E3-ligase optisch te regelen, waardoor de ubiquitinatie en proteasomale afbraak van het doeleiwit werd gecontroleerd.
We demonstreren brede toepasbaarheid door deze benadering toe te passen op een various reeks doeleiwitten, waaronder EGFP, vuurvliegluciferase, de kinase MEK1 en de fosfatase DUSP6 (ook bekend als MKP3). De gekooide degron kan worden gebruikt met minimale eiwittechnologie en biedt vrijwel volledige, door licht geactiveerde eiwitafbraak op een tijdschaal van seconden tot minuut.